DNA/RNA Synthesis Substrate

Ribonucleic Acid, Transfer from Brewing yeast is tRNA, which is isolated from brewer yeast. Ribonucleic Acid, Transfer from Brewing yeast is arranged in a cloverleaf model in total sequence. Ribonucleic Acid, Transfer from Brewing yeast is utilized as a substrate in reactions with participant of tRNAs.

2′-O,4′-C-Methyleneguanosine (LNA-G) is a reverse guanine analog, where LNA (locked nucleic acid) is a nucleic acid analog. LNA modification can be widely used in various fields, such as effective binding affinity with complementary sequences and stronger nuclease resistance than natural nucleotides, providing great potential for application in disease diagnosis and research. 2'-O,4'-C-Methyleneguanosine is a substrate for KOD DNA polymerase, which incorporates LNA-G nucleotides into growing DNA strands, including consecutive incorporations , to generate full-length extension products.

NTP 25 mM Solution, PCR Grade is a mixed solution composed of 25 mM ATP (HY-B2176), 25 mM CTP (HY-125818), 25 mM GTP (HY-113225) and 25 mM UTP (HY-107372), free of DNase, RNase, phosphatase and protease. NTP 25 mM Solution, PCR Grade serves as an energy substrate and raw material for nucleic acid synthesis (DNA/RNA), and can also regulate the advanced structure of DNA to turn transcription on or off.

DMTr-Ac-5-Fluoro-2'-deoxycytidine-phosphoramidite is the phosphoramidite of 5-Fluoro-2'-deoxycytidine (HY-116217). DMTr-Ac-5-Fluoro-2'-deoxycytidine-phosphoramidite enables the direct automated incorporation of 5-fluoro-2'-deoxycytidine into deoxyoligonucleotides via standard phosphoramidite chemistry, allowing the preparation of deoxyoligonucleotides containing 5-fluoro-2'-deoxycytidine.

5-Aza-dCTP tetraTEA (5-Aza-2'-deoxycytidine-5′-triphosphate tetraTEA) is a nucleotide analog that acts as a substrate for mammalian DNA polymerase α (K_m = 3.0 μM) and a weak competitive inhibitor of this enzyme (K_i = 4.3 μM). 5-Aza-dCTP tetraTEA inhibits DNA polymerase α via incorporation into DNA through Watson-Crick base pairing, inhibits DNA methyltransferases via incorporation into hemimethylated DNA, and induces mutations in HIV-1 via incorporation into viral DNA during synthesis. 5-Aza-dCTP tetraTEA reverses the inhibitory effect of dTTP on dCMP deaminase, and allosterically activates dCMP deamination to the same extent as dCTP. 5-Aza-dCTP tetraTEA is applicable to research related to leukemia and type 1 human immunodeficiency virus infection.

F-ara-EdU is a low-toxic, highly stable probe for DNA synthesis that is used as a marker for cell proliferation and DNA replication by binding to DNA or RNA in cells. F-ara-EdU can also be used to detect the rate of DNA synthesis within cells, as well as to study the mechanisms of DNA repair and damage.

5-Aza-dCTP (5-Aza-2'-deoxycytidine-5′-triphosphate) is a nucleotide analog that acts as a substrate for mammalian DNA polymerase α (K_m = 3.0 μM) and a weak competitive inhibitor of this enzyme (K_i = 4.3 μM). 5-Aza-dCTP inhibits DNA polymerase α via incorporation into DNA through Watson-Crick base pairing, inhibits DNA methyltransferases via incorporation into hemimethylated DNA, and induces mutations in HIV-1 via incorporation into viral DNA during synthesis. 5-Aza-dCTP reverses the inhibitory effect of dTTP on dCMP deaminase, and allosterically activates dCMP deamination to the same extent as dCTP. 5-Aza-dCTP is applicable to research related to leukemia and type 1 human immunodeficiency virus infection.