BacPROTAC-1
Based on 1 Customer Validation
BacPROTAC-1 is a BacPROTAC degrader that induces the degradation of the target protein mSA by recruiting ClpC/ClpC1. BacPROTAC-1 binds to mSA, Bacillus subtilis ClpCNTD and Mycobacterium smegmatis ClpC1NTD with Kd values of 3.9, 2.8 and 0.69 μM, respectively. BacPROTAC-1 forms a ternary complex between the substrate and ClpC, promotes ClpC reassembly and activation, and induces ATP-dependent degradation of mSA and mSA fusion model substrates via the ClpCP or ClpC1P1P2 protease system. BacPROTAC-1 can be used in studies on bacterial targeted protein degradation, bacterial protein homeostasis and antibacterial target discovery.
(Pink: Microbial Metabolite ligand (HY-B0511); Blue: ClpC ligand (HY-175571); Black: linker).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 93.46%
- 分子式: C45H83N12O17PS
- 分子量:1127.25
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
KD: 2.8 μM (ClpCNTD), 3.9 μM (mSA)[1]
BacPROTAC-1 (400 μM; syringe load) binds purified Bacillus subtilis ClpCNTD with a KD of 2.8 μM[1].
BacPROTAC-1 (400 μM; syringe load) binds purified monomeric streptavidin (mSA) with a KD of 3.9 μM[1].
BacPROTAC-1 (400 μM; syringe load) binds purified Mycobacterium smegmatis ClpC1NTD with a Kd of 0.69 μM[1].
BacPROTAC-1 (25 μM) drives formation of a stable ternary complex between purified Bacillus subtilis ClpCNTD and monomeric streptavidin (mSA)[1].
BacPROTAC-1 (1-100 μM; 2 h at 37°C) induces degradation of monomeric streptavidin (mSA) by reconstituted Bacillus subtilis ClpCP, with efficient degradation observed at 100 μM after 2 hours at 37°C[1].
BacPROTAC-1 (0.1-100 μM; 2 h at 37°C) potently induces degradation of mSA-Kre by reconstituted Bacillus subtilis ClpCP, with efficient degradation observed at 1 μM after 2 hours at 37°C, and this activity is ATP-dependent and blocked by biotin or pArg[1].
BacPROTAC-1 (1-100 μM; 2 h at 37°C) induces degradation of mSA-NrdI, mSA-TagD, and mSA-NusA by reconstituted Bacillus subtilis ClpCP, but with lower efficiency than mSA-Kre, after 2 hours at 37°C[1].
BacPROTAC-1 (156 μM) induces conversion of inactive decameric Bacillus subtilis ClpCDWB into an active tetramer of hexamers when bound to mSA-Kre[1].
BacPROTAC-1 (156 μM) alone induces partial conversion of inactive decameric Bacillus subtilis ClpCDWB into an active tetramer of hexamers[1].
BacPROTAC-1 (25 μM) drives formation of a stable ternary complex between purified Mycobacterium smegmatis ClpC1NTD and monomeric streptavidin (mSA)[1].
BacPROTAC-1 (0.1-100 μM; 2 h at 37°C) potently induces degradation of mSA-Kre by reconstituted Mycobacterium smegmatis ClpC1P1P2, with efficient degradation observed at 1 μM after 2 hours at 37°C, and this activity is ATP-dependent and blocked by biotin or pArg[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Solid
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分子量 1127.25
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分子式 C45H83N12O17PS
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Color White to off-white
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SMILES
O=C(NCCCC[C@H](NC(CCOCCOCCNC(CCOCCOCCNC(CCOCCOCCNC([C@H](CCCNC(NP(O)(O)=O)=N)NC(C)=O)=O)=O)=O)=O)C(N)=O)CCCC[C@@H]1SC[C@@H]([C@@H]1N2)NC2=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
DMSO : 100 mg/mL (88.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
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- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.8871 mL | 4.4356 mL | 8.8711 mL | 22.1779 mL |
| 5 mM | 0.1774 mL | 0.8871 mL | 1.7742 mL | 4.4356 mL | |
| 10 mM | 0.0887 mL | 0.4436 mL | 0.8871 mL | 2.2178 mL | |
| 15 mM | 0.0591 mL | 0.2957 mL | 0.5914 mL | 1.4785 mL | |
| 20 mM | 0.0444 mL | 0.2218 mL | 0.4436 mL | 1.1089 mL | |
| 25 mM | 0.0355 mL | 0.1774 mL | 0.3548 mL | 0.8871 mL | |
| 30 mM | 0.0296 mL | 0.1479 mL | 0.2957 mL | 0.7393 mL | |
| 40 mM | 0.0222 mL | 0.1109 mL | 0.2218 mL | 0.5544 mL | |
| 50 mM | 0.0177 mL | 0.0887 mL | 0.1774 mL | 0.4436 mL | |
| 60 mM | 0.0148 mL | 0.0739 mL | 0.1479 mL | 0.3696 mL | |
| 80 mM | 0.0111 mL | 0.0554 mL | 0.1109 mL | 0.2772 mL |
BacPROTAC-1 関連分類
HY-153575 関連分類
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