Cyanamide
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Cyanamide is a cell division and plant growth inhibitor, as well as an allelochemical derived from Vicia villosa. Cyanamide inhibits root growth and biomass accumulation in a dose-dependent manner by disrupting the formation of mitotic spindles and phragmoplast complexes, reducing the number of mitotic cells and blocking the cell cycle. The effects of Cyanamide are partially reversible after removal from low-concentration environments. Cyanamide is also a specific inhibitor of aldehyde dehydrogenase (ALDH). Although Cyanamide has no direct effect on tumor growth, it can significantly enhance the anti-tumor efficacy of Cyclophosphamide (HY-17420) at non-toxic doses by inhibiting the inactivation of Cyclophosphamide. Cyanamide enables Cyclophosphamide to exert equivalent therapeutic effects at lower doses, effectively inhibiting the growth of primary and metastatic tumors and prolonging the lifespan of tumor-bearing mice. Cyanamide is commonly used in studies related to ha-1 hepatoma and rls lymphosarcoma.
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- CAS 番号: 420-04-2
- 分子式: CH2N2
- 分子量:42.04
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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生物活性
Cyanamide (2-10 mM; 1-6 d) dose-dependently inhibits root growth, fresh weight accumulation, dry weight accumulation and RGR of bulb roots of onion (Allium cepa L.). Complete growth recovery is only observed after treatment with the low concentration (2 mM), while only partial or no recovery is observed after treatment with high concentrations[1].
Cyanamide (2-10 mM; 1, 6 d) increases H2O2 levels in a dose- and time-dependent manner, inducing oxidative stress in bulb roots of onion (Allium cepa L.), with the highest concentration (10 mM) triggering a sharp rise in H2O2 after 1 day of treatment[1].
Cyanamide (2-10 mM; 1-6 d) alters the accumulation of superoxide anions in the root of onion bulbs. Specifically, treatment with 10 mM cyanamide for 1 d induces extensive accumulation of superoxide anions, which subsequently triggers tissue degradation[1].
Cyanamide (2-10 mM; 1-6 d) alters the mitotic index of onion root tip cells, disrupts chromosome morphology and metaphase plate formation, and all mitotic activity and chromosome structures recover completely after removal of this compound[1].
Cyanamide (2-10 mM; 1-6 d) alters the cell cycle distribution of onion root tip cells, increases the proportion of 4C nuclei after short-term treatment, and elevates the G2/G1 ratio during the recovery phase[1].
Cyanamide (2-10 mM; 1 d) disrupts the arrangement of microtubule cytoskeletons in onion root tip meristematic cells at concentrations of 6 mM and 10 mM, while no visible effect on microtubule structure is observed at the 2 mM concentration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Onion (Allium cepa L.) root tip cells
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Concentration:2, 6, 10 mM
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Incubation Time:6 days
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Result:Increased the proportion of 4C nuclei after short-term treatment and increased the G2/G1 ratio during recovery, with the strongest effect observed at 2 mM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:A/Sn mice (male, intramuscular HA-1 hepatocellular tumor transplant); CBA mice (male, intramuscular RLS lymphosarcoma transplant)[2]
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Dosage:70 mg/kg
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Administration:i.p.; 15 minutes prior to cyclophosphamide
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Result:Significantly inhibited growth of primary tumor nodes and intrahepatic metastases in A/Sn mice with HA-1 hepatocellular carcinoma.
Increased lifespan of A/Sn mice with HA-1 hepatocellular carcinoma by at least 80%.
Produced equivalent therapeutic effect (significant tumor growth inhibition and increased lifespan) as a 3-fold higher dose of cyclophosphamide alone in CBA mice with RLS lymphosarcoma.
Showed no effect on tumor growth or animal lifespan when administered alone in both models.
化学情報
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CAS 番号 420-04-2
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性状 Liquid (Density: 1.282 g/cm3)
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分子量 42.04
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分子式 CH2N2
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SMILES
N#CN
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
純度とドキュメンテーション
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取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)