製品番号: HY-121362

Evernic Acid

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Evernic Acid is an orally active thioredoxin reductase 1 (TrxR1) inhibitor and antiproliferative agent. Evernic Acid inhibits the proliferation and migration of human breast cancer cells. Evernic Acid blocks the NF-κB pathway by inhibiting p65 nuclear translocation and IκBα phosphorylation, thereby suppressing downstream inflammatory mediators. Evernic Acid acts as an antioxidant, anti-inflammatory agent and neuroprotective agent, protects neurons from cell death, mitochondrial dysfunction and oxidative stress damage, reduces astrocyte activation, and ameliorates dopaminergic neuron loss and neuroinflammation. Evernic Acid inhibits enoyl reductases FabI and FabZ of Plasmodium falciparum. Evernic Acid downregulates the expression of lasB and rhlA genes in Pseudomonas aeruginosa, inhibits quorum sensing and biofilm formation, and exerts antibacterial activity against Gram-positive bacteria, Gram-negative bacteria and fungi. Evernic Acid is applicable to research related to breast cancer, Parkinson's disease, bacterial infections and fungal infections.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 99.71%
CAS 番号: 537-09-7
分子式: C₁₇H₁₆O₇
分子量:332.30

保管条件:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Evernic Acid 構造式

CAS 番号: 537-09-7

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
- 体内実験
化学情報
溶剤 & 溶解度
- 体外
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

Fungal Metabolite

TrxR アイソフォーム固有の製品をすべて表示 :

TrxR1
TrxR2

Endogenous Metabolite アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
Huh-7	IC₅₀	>100 μM Compound: 1	Cytotoxicity against human Huh7.5 cells after 24 hrs by Cell-titerGlo luminescence assay Cytotoxicity against human Huh7.5 cells after 24 hrs by Cell-titerGlo luminescence assay	[PMID: 23806111]
Huh-7	IC₅₀	77.3 μM Compound: 1	Antiplasmodial activity against liver stage of Plasmodium berghei ANKA expressing GFP infected in human Huh7.5 cells assessed as reduction in PB18S gene expression after 48 hrs by qRT-PCR analysis Antiplasmodial activity against liver stage of Plasmodium berghei ANKA expressing GFP infected in human Huh7.5 cells assessed as reduction in PB18S gene expression after 48 hrs by qRT-PCR analysis	[PMID: 23806111]
KB	IC₅₀	190.4 μM Compound: 1	Cytotoxicity against human KB cells after 72 hrs by Alamar Blue assay Cytotoxicity against human KB cells after 72 hrs by Alamar Blue assay	[PMID: 23806111]

体外実験

Evernic acid (10-150 μg/mL; 24-52 h) exerts dose-dependent antiproliferative effects on human breast cancer MCF-7 and MDA-MB-453 cell lines, with an IC₅₀ of 33.79 μg/mL at 52 h for MCF-7 cells and 121.40 μg/mL at 48 h for MDA-MB-453 cells^[1].
Evernic acid (33.79-121.40 μg/mL; 48-52 h) upregulates TrxR1 gene expression in human breast cancer MCF-7 cells and downregulates TrxR1 gene expression in MDA-MB-453 cells when treated at respective IC₅₀ concentrations for 52 h (MCF-7) and 48 h (MDA-MB-453)^[1].
Evernic acid (33.79-121.40 μg/mL; 12-24 h) inhibits migration of human breast cancer MCF-7 and MDA-MB-453 cell lines when treated at respective IC₅₀ concentrations, with significant inhibition observed at 24 h for MCF-7 cells and at 12 and 24 h for MDA-MB-453 cells^[1].
Evernic acid (33.79-121.40 μg/mL; 48-52 h) suppresses intracellular TrxR1 enzyme activity by 25% in human breast cancer MCF-7 cells and 30% in MDA-MB-453 cells when treated at respective IC₅₀ concentrations for 52 h (MCF-7) and 48 h (MDA-MB-453)^[1].
Evernic acid (1-100 μM; 6 h pre-incubation followed by 24 h co-treatment with MPP⁺) suppresses MPP⁺-induced apoptotic morphological changes in primary rat cortical neurons, and 100 μM evernic acid normalizes MPP⁺-altered Bcl-2/Bax protein expression balance^[2].
Evernic acid (100 μM; 6 h pre-incubation followed by 6 h co-treatment with MPP⁺) protects primary rat cortical neurons from MPP⁺-induced mitochondrial membrane potential loss^[2].
Evernic acid (0.1-100 μM; 6 h pre-incubation prior to MPP⁺ co-treatment) dose-dependently suppresses MPP⁺-induced ROS generation in primary rat cortical neurons^[2].
Evernic acid (100 μM; 6 h pre-incubation followed by 1 h or 24 h co-treatment with MPP⁺) inhibits the NF-κB signaling pathway in primary rat astrocytes exposed to MPP⁺, by blocking p65 nuclear translocation, reducing IκBα phosphorylation, and lowering COX-2 expression^[2].
Evernic acid (100 μM; 6 h pre-incubation prior to MPP⁺ co-treatment) suppresses MPP⁺-induced increases in pro-inflammatory cytokine (IL-1β, IL-6, TNF-α) and chemokine (CCL2) mRNA expression in primary rat astrocytes^[2].
Evernic acid (as the primary component of fraction VI) inhibits the growth of Staphylococcus aureus ATCC 29213 (MIC 0.98 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC 31.25 μg/mL), Escherichia coli ATCC 25922 (MIC 125 μg/mL), and Candida albicans ATCC 90028 (MIC 62.5 μg/mL) in vitro^[3].
Evernic acid inhibits the expression of quorum-sensing-dependent virulence genes lasB and rhlA in Pseudomonas aeruginosa in vitro^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-453 cell lines
Concentration:

10-150 μg/mL (24 h); 10-100 μg/mL (52 h, MCF-7); 10-150 μg/mL (48 h, MDA-MB-453)
Incubation Time:

24 h; 52 h (MCF-7); 48 h (MDA-MB-453)
Result:

Suppressed the proliferation of MCF-7 and MDA-MB-453 cells in a dose-dependent manner.
Resulted in an IC₅₀ value of 33.79 μg/mL in MCF-7 cells treated for 52 h.
Resulted in an IC₅₀ value of 121.40 μg/mL in MDA-MB-453 cells treated for 48 h.

Apoptosis Analysis^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-453 cell lines
Concentration:

33.79 μg/mL (MCF-7, IC₅₀); 121.40 μg/mL (MDA-MB-453, IC₅₀)
Incubation Time:

52 h (MCF-7); 48 h (MDA-MB-453)
Result:

Did not change the percentage of apoptotic and necrotic cells in MCF-7 cells compared with the control group.
Caused a very low increase in the percentage of late apoptotic cells but did not affect early apoptotic and necrotic cell percentages in MDA-MB-453 cells.

Real Time qPCR^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-453 cell lines
Concentration:

33.79 μg/mL (MCF-7, IC₅₀); 121.40 μg/mL (MDA-MB-453, IC₅₀)
Incubation Time:

52 h (MCF-7); 48 h (MDA-MB-453)
Result:

Downregulated BAX, BCL2, and BAX/BCL2 ratio gene expression by 4.3-, 2.5-, and 2.4-fold, respectively, but did not change P53 gene expression in MCF-7 cells.
Downregulated BAX, BCL2, P53, and BAX/BCL2 ratio gene expression by 2.1-, 1.2-, 1.9-, and 1.7-fold, respectively, in MDA-MB-453 cells.\nUpregulated TrxR1 mRNA expression by 2-fold compared to the control group in MCF-7 cells.
Downregulated TrxR1 mRNA expression by 1.5-fold compared to the control group in MDA-MB-453 cells.

Western Blot Analysis^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-453 cell lines
Concentration:

33.79 μg/mL (MCF-7, IC₅₀); 121.40 μg/mL (MDA-MB-453, IC₅₀)
Incubation Time:

52 h (MCF-7); 48 h (MDA-MB-453)
Result:

Did not change the protein expression of BAX or P53 in MCF-7 cells.
Suppressed P53 protein expression by 5.8-fold but did not change BAX protein expression in MDA-MB-453 cells.\nDid not alter TrxR1 protein expression in either MCF-7 or MDA-MB-453 cells compared to control groups.

Cell Migration Assay^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-453 cell lines
Concentration:

33.79 μg/mL (MCF-7, IC₅₀); 121.40 μg/mL (MDA-MB-453, IC₅₀)
Incubation Time:

12 h, 24 h
Result:

Reduced wound closure to 30.0% at 24 h in MCF-7 cells, compared to 38.8% in the control group.
Reduced wound closure to 0.7% at 12 h and resulted in a -4.0% wound closure (negative value indicates wound enlargement) at 24 h in MDA-MB-453 cells, compared to 4.3% and 12.6% in the control group, respectively.

体内実験

Evernic acid (5-80 mg/kg; p.o.; daily; 10 days) significantly accelerates motor function recovery, attenuates dopaminergic neuronal loss, and suppresses astroglial activation in MPTP-induced Parkinson’s disease model mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (male, 6 weeks old, 20-23 g, MPTP-induced Parkinson's disease)^[2]
Dosage:

5 mg/kg; 80 mg/kg
Administration:

p.o.; daily; 10 days
Result:

Significantly accelerated recovery of motor function after MPTP-induced impairment, as measured by rotarod testing (80 mg/kg).
Significantly attenuated MPTP-induced dopaminergic neuronal loss in both the striatum (STR) and substantia nigra (SN), as quantified by tyrosine hydroxylase (TH) densitometry in the STR and TH-positive neuron counts in the SN (80 mg/kg).
Significantly suppressed MPTP-induced astroglial activation in the STR and SN, as measured by GFAP fluorescence intensity; no effect on MPTP-induced microglial activation was observed (80 mg/kg).
Showed partial, less pronounced protective effects on dopaminergic neuronal loss and astroglial activation compared to the 80 mg/kg dose (5 mg/kg).

化学情報

CAS 番号 537-09-7
性状 Solid
分子量 332.30
分子式 C₁₇H₁₆O₇
Color White to off-white
SMILES

CC1=CC(OC(C2=C(C=C(C=C2O)OC)C)=O)=CC(O)=C1C(O)=O
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- other families
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 10 mg/mL (30.09 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0093 mL

15.0466 mL

30.0933 mL

5 mM

0.6019 mL

3.0093 mL

6.0187 mL

10 mM

0.3009 mL

1.5047 mL

3.0093 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (285 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Kalın ŞN, et al. Effect of evernic acid on human breast cancer MCF-7 and MDA-MB-453 cell lines via thioredoxin reductase 1: A molecular approach. J Appl Toxicol. 2023;43(8):1148-1158. [Content Brief]

[2]. Lee S, et al. Neuroprotective and Anti-Inflammatory Effects of Evernic Acid in an MPTP-Induced Parkinson's Disease Model. Int J Mol Sci. 2021;22(4):2098. Published 2021 Feb 20. [Content Brief]

[3]. Shcherbakova A, et al. Antimicrobial and antioxidant activity of Evernia prunastri extracts and their isolates. World J Microbiol Biotechnol. 2021;37(8):129. Published 2021 Jul 7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0093 mL	15.0466 mL	30.0933 mL	75.2332 mL
	5 mM	0.6019 mL	3.0093 mL	6.0187 mL	15.0466 mL
	10 mM	0.3009 mL	1.5047 mL	3.0093 mL	7.5233 mL
	15 mM	0.2006 mL	1.0031 mL	2.0062 mL	5.0155 mL
	20 mM	0.1505 mL	0.7523 mL	1.5047 mL	3.7617 mL
	25 mM	0.1204 mL	0.6019 mL	1.2037 mL	3.0093 mL
	30 mM	0.1003 mL	0.5016 mL	1.0031 mL	2.5078 mL

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Evernic Acid

Antioxidant Compound Library

Oxygen Sensing Compound Library

Antibacterial Compound Library

Pyroptosis Compound Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Microbial Metabolite Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

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Anti-Pulmonary Fibrosis Compound Library

Metabolic Enzyme Compound Library

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Bioactive Compound Library Max

MCE Bioactive Compound Library

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Anti-Fibrosis Compound Library

Miao Ethnicity Medicine Compound Library

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High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

TrxR アイソフォーム固有の製品をすべて表示

Endogenous Metabolite アイソフォーム固有の製品をすべて表示

生物活性

化学情報

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Evernic Acid 関連分類

HY-121362 関連分類