製品番号: HY-144835

Camibirstat

(別名: FHD-286)

3 Cited Publications

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

FHD-286 is a selective, oral inhibitor of SMARCA4/SMARCA2 ATPase (BRG1 and BRM) inhibitor. FHD-286 has the potential for the research of BAF (BRG1/BRM-associated factor)-related disorders such as acute myeloid leukemia.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 98.79%
CAS 番号: 2671128-05-3
分子式: C₂₄H₃₀N₆O₆S₂
分子量:562.66

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Camibirstat 構造式

CAS 番号: 2671128-05-3

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

SWI/SNF Complex アイソフォーム固有の製品をすべて表示 :

体外実験

FHD-286 (10 to 100 nM) for 7 days induces differentiation followed by loss of viability of AML cell lines and PD AML cells with MLL rearrangement (r), mutant (mt) NPM1 and chromosome 3q26 lesions (with EVI1 overexpression). Treatment with FHD-286 causes whole-genome, concordant, up- or down-regulations in ATAC-Seq peaks and RNA-Seq-determined mRNA expressions of specific loci, associated with significant reduction in the gene-sets of targets of MYC, mTORC1, E2F, Interferon-gamma, IL6-JAK-STAT3, as well as of inflammatory response and oxidative phosphorylation genes. QPCR analyses determined significant reduction in mRNA expression of MYC, SPI1 and BCL2 genes. Mass spectrometry on AML cell lysates treated with FHD-286 showed log2 fold-reductions in c-Myc, SPI1, MEF2C, KMT2C and CDK4 (in MOLM13) and in EVI1, c-Myb, CDK6 and c-Myc (in AML191) cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

FHD-286 (1.5 mg/kg; oral administration; for 10 days) increases in IFNγ and Th1-type chemokine CXCL10 levels^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

B16F10 tumor-bearing mice^[2]
Dosage:

1.5 mg/kg
Administration:

Oral administration; for 10 days
Result:

Increased in IFNγ and Th1-type chemokine CXCL10 levels.

臨床実験

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

化学情報

CAS 番号 2671128-05-3
性状 Solid
分子量 562.66
分子式 C₂₄H₃₀N₆O₆S₂
Color Off-white to light yellow
SMILES

COC[C@@H](C(NC1=NC(C2=CC=CC(N3C[C@@H](O[C@@H](C3)C)C)=N2)=CS1)=O)NC(C4=CN(C=C4)S(C)(=O)=O)=O
別名

FHD-286
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

All Cell Imaging/StainingIFFlow CytometryCell Proliferation/Viability Assay

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Journal Impact Factor

Mol Cell

β-catenin functions as a molecular adapter for disordered cBAF interactions. [Abstract]

2025 Aug 21;85(16):3041-3056.e9. PMID: 40695292
bioRxiv

Spatially-Resolved Multiomic Atlas of Leiomyosarcoma Identifies Two Clinically Relevant Epigenetically-Driven Cell States. [Abstract]

2026 May 26:2026.05.22.726988. PMID: 42244620
bioRxiv

2024 Mar 29.

Camibirstat purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Mar 29.

HeLa cells were pre-treated with or without 1 μM FHD286 for two hours. Laser microirradiation was performed to examine the localization of SMARCA2, SMARCC1 and SMARCD1.

Camibirstat purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Mar 29.

Lacking the enzymatic activities of SMARCA2/4 extends the accumulation of γH2AX, RNF8 and BRCA1. HeLa cells were pre-treated with or without FHD286 (1 μM) or Compound 14 (1 μM) for two hours followed by 5 Gy of IR. Following the indicated recovery time, the foci of γH2AX, RNF8 and BRCA1 were examined by IF.

Camibirstat purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Mar 29.

HR efficacy was examined by GFP reporter assays. U2OS cells with DR-GFP insertion were pretreated with or without FHD286 (1 μM) or Compound 14 (1 μM). I-SceI were expressed to induce DSBs. GFP positive populations were calculated by flowcytometry.

Camibirstat purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Mar 29.

1000 cells were seeded in each well, and treated with indicated doses of FHD286 and/or olaparib. Colony formation was examined at Day 14 with 0.1% crystal violet staining. Statistical analyses were shown in the lower panel.

Camibirstat purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Mar 29.

HeLa cells were pre-treated with or without FHD286 (1 μM) or Compound 14 (1 μM) for two hours followed by 5 Gy of IR. Foci formation of RAD51, SMARCA2 were examined.

溶剤 & 溶解度

体外:

DMSO : 250 mg/mL (444.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7773 mL

8.8864 mL

17.7727 mL

5 mM

0.3555 mL

1.7773 mL

3.5545 mL

10 mM

0.1777 mL

0.8886 mL

1.7773 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (3.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (275 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Warren C. Fiskus, et al. Abstract 1140: Pre-clinical efficacy of targeting BAF complexes through inhibition of the dual ATPases BRG1 and BRM by FHD-286 in cellular models of AML. Cancer Res (2023) 83 (7_Supplement): 1140.

[2]. Kana Ichikawa, et al. Synergistic efficacy of the BRM/BRG1 ATPase inhibitor, FHD-286, and anti-PD-1 antibody in mouse syngeneic tumor models.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7773 mL	8.8864 mL	17.7727 mL	44.4318 mL
	5 mM	0.3555 mL	1.7773 mL	3.5545 mL	8.8864 mL
	10 mM	0.1777 mL	0.8886 mL	1.7773 mL	4.4432 mL
	15 mM	0.1185 mL	0.5924 mL	1.1848 mL	2.9621 mL
	20 mM	0.0889 mL	0.4443 mL	0.8886 mL	2.2216 mL
	25 mM	0.0711 mL	0.3555 mL	0.7109 mL	1.7773 mL
	30 mM	0.0592 mL	0.2962 mL	0.5924 mL	1.4811 mL
	40 mM	0.0444 mL	0.2222 mL	0.4443 mL	1.1108 mL
	50 mM	0.0355 mL	0.1777 mL	0.3555 mL	0.8886 mL
	60 mM	0.0296 mL	0.1481 mL	0.2962 mL	0.7405 mL
	80 mM	0.0222 mL	0.1111 mL	0.2222 mL	0.5554 mL
	100 mM	0.0178 mL	0.0889 mL	0.1777 mL	0.4443 mL

Keywords

Camibirstat

3 Cited Publications

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Camibirstat

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

MedChemExpress（MCE）の使用を引用している文献 Camibirstat

SWI/SNF Complex アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (3)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Camibirstat

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

MedChemExpress（MCE）の使用を引用している文献 Camibirstat

SWI/SNF Complex アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (3)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Camibirstat 関連分類

HY-144835 関連分類