KIF18A-IN-4
KIF18A-IN-4 is a moderately potent ATP and microtubule (MT) noncompetitive KIF18A inhibitor (IC50=6.16 μM). KIF18A-IN-4 has selectivity against a large panel of mitotic kinesins and kinases, and does not show any direct effects on tubulin assembly. KIF18A-IN-4 exhibits anti-tumor activity.
For research use only. We do not sell to patients.
- CAS No.: 1197522-21-6
- Formula: C22H27N3O3S
- Molecular Weight:413.53
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Biological Activity
Description
IC50 & Target
IC50: 6.16 μM (KIF18A)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-157 | IC50 |
4.8 μM
Compound: 3
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Induction of mitotic arrest in TP53 mutant human MDA-MB-157 cells assessed as multipolar spindle arrays by measuring pericentriolar material incubated for 24 hrs by mitotic index assay
Induction of mitotic arrest in TP53 mutant human MDA-MB-157 cells assessed as multipolar spindle arrays by measuring pericentriolar material incubated for 24 hrs by mitotic index assay
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[PMID: 35286090] |
| OVCAR-3 | EC50 |
5.03 μM
Compound: 3
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Antiproliferative activity against human OVCAR-3 cells by nuclear count assay
Antiproliferative activity against human OVCAR-3 cells by nuclear count assay
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[PMID: 35286090] |
| OVCAR-3 | EC50 |
6.35 μM
Compound: 3
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Induction of mitotic arrest in human OVCAR-3 cells assessed as p-histone H3 incubated for 24 hrs by mitotic - imaging assay
Induction of mitotic arrest in human OVCAR-3 cells assessed as p-histone H3 incubated for 24 hrs by mitotic - imaging assay
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[PMID: 35286090] |
In Vitro
KIF18A-IN-4 (15 μM; 24 hours) induces a phenotype characterized by multipolar spindle arrays emanating from multiple pericentriolar material (PCM) centers in mitotic MDA-MB-157 cells (pH3+)[1].
KIF18A-IN-4 (0-10 μM; overnight) has an EC50 of 6.35 μM in OVCAR-3 cells by the mitotic index assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1197522-21-6
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Molecular Weight 413.53
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Formula C22H27N3O3S
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SMILES
O=S(C1=CC=CC(NC(C2=C(N3CCCCC3)N=CC=C2)=O)=C1)(C4CCCC4)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
KIF18A-IN-4 Related Classifications
HY-145827 Related Classifications
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