DYRKtide
Based on 1 Customer Validation
DYRKtide is a peptide substrate for DYRK family kinases and can be used to measure DYRK1A substrate phosphorylation and the catalytic activity of DYRK1A mutants. DYRKtide can be used in studies related to in vitro kinase activity assays and in research on DYRK1A-related diseases.
For research use only. We do not sell to patients.
- Purity : 99.57%
- CAS No.: 2250228-51-2
- Formula: C79H135N31O17
- Molecular Weight:1791.11
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
DYRK1A |
DYRK1B |
DYRK2 |
DYRK4 |
In Vitro
DYRKtide is phosphorylated by recombinant DYRK1A, DYRK1B, MNB, DYRK2, and DYRK4, and Harmine (HY-N0737A) inhibits mature DYRK1A-mediated DYRKtide phosphorylation with an IC50 of 0.033 μM[2].
DYRKtide serves as a phospho-acceptor substrate for determining DYRKs activity in cell-free DYRKs kinase assays[3].
DYRKtide is phosphorylated by wild-type DYRK1A[4].
DYRKtide is a substrate peptide in the cell-free DYRK1A Cook assay, and its DYRK1A-mediated phosphorylation can be inhibited by the tested small molecules, with Ki values below 5 µM[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2250228-51-2
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Appearance Solid
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Molecular Weight 1791.11
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Formula C79H135N31O17
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Color White to off-white
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Sequence
Arg-Arg-Arg-Phe-Arg-Pro-Ala-Ser-Pro-Leu-Arg-Gly-Pro-Pro-Lys
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Sequence Shortening
RRRFRPASPLRGPPK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (55.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5583 mL | 2.7916 mL | 5.5831 mL | 13.9578 mL |
| 5 mM | 0.1117 mL | 0.5583 mL | 1.1166 mL | 2.7916 mL | |
| 10 mM | 0.0558 mL | 0.2792 mL | 0.5583 mL | 1.3958 mL | |
| 15 mM | 0.0372 mL | 0.1861 mL | 0.3722 mL | 0.9305 mL | |
| 20 mM | 0.0279 mL | 0.1396 mL | 0.2792 mL | 0.6979 mL | |
| 25 mM | 0.0223 mL | 0.1117 mL | 0.2233 mL | 0.5583 mL | |
| 30 mM | 0.0186 mL | 0.0931 mL | 0.1861 mL | 0.4653 mL | |
| 40 mM | 0.0140 mL | 0.0698 mL | 0.1396 mL | 0.3489 mL | |
| 50 mM | 0.0112 mL | 0.0558 mL | 0.1117 mL | 0.2792 mL |
DYRKtide Related Classifications
HY-P5430 Related Classifications
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