8-Bromoadenosine
Based on 1 Customer Validation
8-Bromoadenosine is an adenosine analog. Adenosine analogs mostly act as smooth muscle vasodilators and have also been shown to inhibit cancer progression. 8-Bromoadenosine can quench the intrinsic fluorescence of human serum albumin (HSA) through static quenching procedure. 8-Bromoadenosine can be used to synthesize adenosine phosphate, such as Acadesine (HY-13417), Clofarabine (HY-A0005), Fludarabine phosphate (HY-B0028) and Vidarabine (HY-B0277).
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.73%
- CAS No.: 2946-39-6
- 화학식: C10H12BrN5O4
- 분자량:346.14
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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HY-B1826
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Acyclovir -
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2-Chloroadenine -
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Uridine 5'-monophosphate (Standard) -
HY-17422R
Acyclovir (Standard) -
HY-W007791
Chloropurine riboside -
HY-B0004
Doxofylline -
HY-B0277
Vidarabine -
HY-17425A
Valacyclovir hydrochloride
Biological Activity
제품 설명
In Vitro
8-Bromoadenosine reduces the fluorescence emission intensity of HSA[2].
8-Bromoadenosine binds to HSA with less stability as the temperature increases but the effect of temperature is very small[2].
8-Bromoadenosine has a decreased binding constants with HSA in the presence of other ions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2946-39-6
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Appearance Solid
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분자량 346.14
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화학식 C10H12BrN5O4
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Color Yellow to orange
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(Br)=NC3=C2N=CN=C3N)O[C@@H]1CO
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
DMSO : 100 mg/mL (288.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (14.45 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (277 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Man S, et al. Potential and promising anticancer drugs from adenosine and its analogs. Drug Discov Today. 2021 Jun;26(6):1490-1500. [Content Brief]
[2]. Cui, F., (2009). Characterization of the interaction between 8-bromoadenosine with human serum albumin and its analytical application. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 74(4), 964–971. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8890 mL | 14.4450 mL | 28.8900 mL | 72.2251 mL |
| 5 mM | 0.5778 mL | 2.8890 mL | 5.7780 mL | 14.4450 mL | |
| 10 mM | 0.2889 mL | 1.4445 mL | 2.8890 mL | 7.2225 mL | |
| 15 mM | 0.1926 mL | 0.9630 mL | 1.9260 mL | 4.8150 mL | |
| 20 mM | 0.1445 mL | 0.7223 mL | 1.4445 mL | 3.6113 mL | |
| 25 mM | 0.1156 mL | 0.5778 mL | 1.1556 mL | 2.8890 mL | |
| 30 mM | 0.0963 mL | 0.4815 mL | 0.9630 mL | 2.4075 mL | |
| 40 mM | 0.0722 mL | 0.3611 mL | 0.7223 mL | 1.8056 mL | |
| 50 mM | 0.0578 mL | 0.2889 mL | 0.5778 mL | 1.4445 mL | |
| 60 mM | 0.0482 mL | 0.2408 mL | 0.4815 mL | 1.2038 mL | |
| 80 mM | 0.0361 mL | 0.1806 mL | 0.3611 mL | 0.9028 mL | |
| 100 mM | 0.0289 mL | 0.1445 mL | 0.2889 mL | 0.7223 mL |
8-Bromoadenosine Related Classifications
HY-W013195 Related Classifications
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