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Cat. No.: HY-123056

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EAD1

EAD1 is a Chloroquine (HY-17589A) analog with anticancer activity. EAD1 blocks autophagy, leading to the intracellular accumulation of autophagosome-related proteins LC3-II and p62. EAD1 induces cancer cell apoptosis and inhibits cancer cell proliferation. EAD1 can be used in the research of lung cancer and pancreatic cancer.

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CAS No.: 1644388-26-0
화학식: C₂₄H₂₇Cl₂N₇
분자량:484.42

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

EAD1 Chemical Structure

CAS No.: 1644388-26-0

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

Cellular Effect

Cell Line	Type	Value	Description	References
BXPC-3	IC₅₀	5.8 μM Compound: 3h, EAD1	Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method Antiproliferative activity against human BxPC3 cells after 72 hrs by SRB method	[PMID: 25699157]
HCC827	IC₅₀	7.6 μM Compound: 3h, EAD1	Antiproliferative activity against human HCC827 cells after 72 hrs by SRB method Antiproliferative activity against human HCC827 cells after 72 hrs by SRB method	[PMID: 25699157]
NCI-H460	IC₅₀	11 μM Compound: 3h, EAD1	Antiproliferative activity against human H460 cells after 72 hrs by SRB method Antiproliferative activity against human H460 cells after 72 hrs by SRB method	[PMID: 25699157]

In Vitro

EAD1 (compound 3h) potently inhibits the proliferation of H460, HCC827 and BxPC3 cancer cells at 72 h, with IC₅₀ values of 11 μM, 7.6 μM and 5.8 μM, respectively^[1].
EAD1 (6.5-100 μM; 24 h) potently inhibits colony formation of H460 non-small cell lung cancer cells^[1].
EAD1 (5-75 μM; 24 h) induces concentration-dependent apoptosis in H460 non-small cell lung cancer (NSCLC) cells^[1].
EAD1 (25-50 μM; 6 h) enhances the expression of punctate LC3 in H3122 non-small cell lung cancer cells^[1].
EAD1 (1-25 μM, 10 μM; 2-72 h, 24 h) induces concentration- and time-dependent accumulation of autophagy-related proteins LC3-II and p62 in H460 non-small cell lung cancer (NSCLC) cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

H460 human NSCLC cells
Concentration:

6.5, 13, 25, 50, 100 μM
Incubation Time:

24 h
Result:

Inhibited colony formation of H460 non-small cell lung cancer cells.

Apoptosis Analysis^[1]

Cell Line:

H460 human NSCLC cells
Concentration:

5, 25, 50, 75 μM
Incubation Time:

24 h
Result:

Induced apoptosis in H460 cells in a concentration-dependent manner.

Western Blot Analysis^[1]

Cell Line:

H460 human NSCLC cells
Concentration:

1, 2.5, 5, 10, 25 μM (24 h incubation); 10 μM (time-course incubation)
Incubation Time:

2, 4, 6, 24, 48, 72 h (10 μM concentration); 24 h (1-25 μM concentration)
Result:

Caused concentration- and time-dependent increases in autophagosome-bound LC3-II levels.
Showed larger increase in LC3-II levels at each tested concentration compared to HCQ.
Made the effect of 25 μM HCQ on LC3-II approximately equal to that of 5 μM EAD1.
Caused concentration-dependent accumulation of p62 (an autophagy substrate), with more potent activity than HCQ.

Related Protocols :

Chemical Information

CAS No. 1644388-26-0
분자량 484.42
화학식 C₂₄H₂₇Cl₂N₇
SMILES

CN(CCNCC1=CN(CC2=CC=C(Cl)C=C2)N=N1)CCNC3=CC=NC4=CC(Cl)=CC=C34
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lars Ulrik Nordstrøm, et al. Discovery of Autophagy Inhibitors With Antiproliferative Activity in Lung and Pancreatic Cancer Cells. ACS Med Chem Lett. 2015 Jan 8;6(2):134-9. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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