Isonicotinamide
Based on 1 Customer Validation
Isonicotinamide is a structural isomer of Nicotinamide (HY-B0150). Isonicotinamide promotes the activity of the NAD+-dependent deacetylase Sir2 in yeast and affects NAD+ homeostasis and nucleotide salvage synthesis pathways. Isonicotinamide can also serve as an intermediate for the synthesis of active drugs.
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- Purity : 98.0%
- CAS No.: 1453-82-3
- 화학식: C6H6N2O
- 분자량:122.12
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보관:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCCLM3 | IC50 |
500 μM
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Cytotoxicity against murine LM3 breast tumor cells with IC50 >500 μM assessed as viability reduction after 24 hrs by MTT assay.
Cytotoxicity against murine LM3 breast tumor cells with IC50 >500 μM assessed as viability reduction after 24 hrs by MTT assay.
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20724041 |
In Vitro
Isonicotinamide (25-125 mM; day 2) high concentrations above 50 mM inhibit BY4741 growth, with 125 mM causing strong early growth reduction[1].
Isonicotinamide (25-125 mM; 2 days) sensitivity across the yeast knockout collection is dose-dependent, with 150-326 mutants sensitive at 25-125 mM and 341 genes from the 25-75 mM conditions used for pathway enrichment[1].
Isonicotinamide (25-75 mM; day 2) sensitivity occurs in htz1Δ and SWR1 complex mutants, with htz1Δ sensitive at 25 mM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Isonicotinamide (976 mg/kg/day; intraperitoneal; daily; 10 days) does not prolong survival in L1210G leukemia as a single agent, but in combination with ATDA at 33.7 or 16.85 mg/kg/day it increases survival time[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:F1 hybrids of the Bagg Albino × C58 cross[2]
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Dosage:244, 61, and 60 mg/kg/day alone
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Administration:intraperitoneal; daily; 10 days
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Result:At 244 mg/kg/day alone, tumor weight was 475 mg and inhibition was 34%.
At 61 mg/kg/day alone, tumor weight was 677 mg and inhibition was 6%.
At 60 mg/kg/day alone, tumor weight was 543 mg and inhibition was 12%.
With ATDA 7.5 mg/kg/day, at 244, 122, and 61 mg/kg/day, tumor weight was 0 mg and inhibition was 100%.
With ATDA 7.5 mg/kg/day at 30.5 mg/kg/day, tumor weight was 12 mg and inhibition was 98%.
With ATDA 7.5 mg/kg/day at 15.25 mg/kg/day, tumor weight was 266 mg and inhibition was 63%.
With ATDA 7.5 mg/kg/day at 60 mg/kg/day, tumor weight was 27 mg and inhibition was 96%.
With ATDA 3.75 mg/kg/day and 60 mg/kg/day, tumor weight was 256 mg and inhibition was 58%.
With ATDA 1.875 mg/kg/day and 60 mg/kg/day, tumor weight was 325 mg and inhibition was 47%.
In the separate B82T experiment, ATDA 7.5 mg/kg/day with 122 mg/kg/day produced tumor weight 2 mg and 100% inhibition.
In the separate B82T experiment, ATDA 7.5 mg/kg/day with 61 mg/kg/day produced tumor weight 34 mg and inhibition 96%.
Chemical Information
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CAS No. 1453-82-3
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Appearance Solid
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분자량 122.12
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화학식 C6H6N2O
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Color White to off-white
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SMILES
NC(C1=CC=NC=C1)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
용액&용해도
In Vitro:
DMSO : ≥ 200 mg/mL (1637.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (40.94 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (40.94 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (289 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.1887 mL | 40.9433 mL | 81.8867 mL | 204.7167 mL |
| 5 mM | 1.6377 mL | 8.1887 mL | 16.3773 mL | 40.9433 mL | |
| 10 mM | 0.8189 mL | 4.0943 mL | 8.1887 mL | 20.4717 mL | |
| 15 mM | 0.5459 mL | 2.7296 mL | 5.4591 mL | 13.6478 mL | |
| 20 mM | 0.4094 mL | 2.0472 mL | 4.0943 mL | 10.2358 mL | |
| 25 mM | 0.3275 mL | 1.6377 mL | 3.2755 mL | 8.1887 mL | |
| 30 mM | 0.2730 mL | 1.3648 mL | 2.7296 mL | 6.8239 mL | |
| 40 mM | 0.2047 mL | 1.0236 mL | 2.0472 mL | 5.1179 mL | |
| 50 mM | 0.1638 mL | 0.8189 mL | 1.6377 mL | 4.0943 mL | |
| 60 mM | 0.1365 mL | 0.6824 mL | 1.3648 mL | 3.4119 mL | |
| 80 mM | 0.1024 mL | 0.5118 mL | 1.0236 mL | 2.5590 mL | |
| 100 mM | 0.0819 mL | 0.4094 mL | 0.8189 mL | 2.0472 mL |
Isonicotinamide Related Classifications
HY-W012843 Related Classifications
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