Cat. No.: HY-19527

IST5-002

(Synonyms: N6-Benzyladenosine-5'-phosphate)

1 Cited Publications

고객리뷰

Based on 1 publication(s) in Google Scholar

IST5-002, a potent Stat5a/b inhibitor, selectively inhibits transcriptional activity of Stat5a/b (IC₅₀s: 1.5 μM for Stat5a, 3.5 μM for Stat5b). IST5-002 inducs cell apoptotic and death of prostate cancer cells and chronic myeloid leukemia (CML) cells. IST5-002 can be used in the research of prostate cancer and chronic myeloid leukemia (CML).

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.83%
CAS No.: 13484-66-7
화학식: C₁₇H₂₀N₅O₇P
분자량:437.34

보관:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

IST5-002 Chemical Structure

CAS No.: 13484-66-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target^[1]

STAT5a

1.5 μM (IC₅₀)

STAT5b

3.5 μM (IC₅₀)

All STAT Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	7.7 μM Compound: BAMP	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 25108359]
HCT-15	IC₅₀	0.8 μM Compound: BAMP	Cytotoxicity against human HCT15 cells after 72 hrs by MTT assay Cytotoxicity against human HCT15 cells after 72 hrs by MTT assay	[PMID: 25108359]
HL-60	IC₅₀	5 μM Compound: BAMP	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 25108359]

In Vitro

IST5-002 (1.5-25 μM, 2 h) inhibits transcriptional activity of Stat5a and Stat5b in a dose-dependent manner^[1].
IST5-002 (0-40 μM, 3 h) inhibits Bcr-Abl-induced Stat5a/b phosphorylation in K562 cells^[1].
IST5-002 (5-100 μM, 2 h) inhibits Stat5a/b phosphorylation in T47D cells, and inhibits dimerization in PC-3 cells^[1].
IST5-002 (5-100 μM, 2 h) suppresses Stat5 nuclear translocation in PC-3 cells, and inhibits DNA binding of Stat5 target genes and COS-7 cells^[1].
IST5-002 (2-50 μM, 48 h) reduces expression of Stat5a/b target genes (Bcl-xL and cyclin D1) in CWR22Rv1 and LNCaP cells^[1].
IST5-002 (3.1-50 μM, 72 h) inhibits cell growth through induction of apoptosis in human prostate cancer cells^[1].
IST5-002 (25-100 μM, 7 days) induces epithelial cell death in patient-derived prostate cancers ex vivo in organ explant cultures^[1].
IST5-002 (5 μM, 24-72h) inhibits Stat5a/b phosphorylation and induces apoptosis of Imatinib (HY-15463)-sensitive and -resistant CML cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

CWR22Rv1, LNCaP, and DU145 cells
Concentration:

3.1, 6.3, 12.5, 25, 50 μM
Incubation Time:

72 h
Result:

Decreased viable cells by 50% to 80% at 12.5 μM.

Cell Cycle Analysis^[1]

Cell Line:

LNCaP and CWR22Rv1 cells
Concentration:

6, 12, 25 μM
Incubation Time:

72 h
Result:

Increased the fraction of dead cells (sub-G1) and decreased the fraction of living cells (G2–M).

Western Blot Analysis^[1]

Cell Line:

Bcr-Abl–positive K562 cells
Concentration:

0, 1, 5, 10, 20, 40 μM
Incubation Time:

3 h
Result:

Inhibited Bcr-Abl-induced Stat5a/b phosphorylation at 5 μM, without affecting Bcr-Abl tyrosine phosphorylation levels.

Immunofluorescence^[1]

Cell Line:

PC-3 cells
Concentration:

5, 10, 15. 20, 40 μM
Incubation Time:

2 h
Result:

Inhibited Prl (Prolactin)-induced nuclear translocation of Stat5.

In Vivo

RORγt inverse agonist 29 (intraperitoneal injection, 25-100 mg/kg, daily for 10 days) inhibits tumor growth in prostate cancer xenograft model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Prostate cancer (CWR22Rv1) xenograft model^[1]
Dosage:

25, 50, and 100 mg/kg
Administration:

Intraperitoneal injection, daily for 10 days
Result:

Induced massive loss of viable tumor cells and dead rounded cells accumulation.
Induced cell death through apoptosis (shown by fragmented DNA in tumor sections).
Decreased nuclear Stat5a/b content by 60%, 78%, and 90% at 25, 50, and 100 mg/kg, respectively.

Chemical Information

CAS No. 13484-66-7
Appearance Solid
분자량 437.34
화학식 C₁₇H₂₀N₅O₇P
Color White to off-white
SMILES

O=P(O)(OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC3=C(N=CN=C32)NCC4=CC=CC=C4)O1)O)O)O
Synonyms

N6-Benzyladenosine-5'-phosphate
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Exp Eye Res

Targeting STAT5 attenuates retinal neovascularization by promoting apoptosis and suppressing endothelial cell function. [Abstract]

2026 Feb:263:110788. PMID: 41349784

용액&용해도

In Vitro:

DMSO : 125 mg/mL (285.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 125 mg/mL (285.82 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2866 mL

11.4328 mL

22.8655 mL

5 mM

0.4573 mL

2.2866 mL

4.5731 mL

10 mM

0.2287 mL

1.1433 mL

2.2866 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhiyong Liao, et al. Structure-Based Screen Identifies a Potent Small Molecule Inhibitor of Stat5a/b with Therapeutic Potential for Prostate Cancer and Chronic Myeloid Leukemia. Mol Cancer Ther. 2015 Aug;14(8):1777-93. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	2.2866 mL	11.4328 mL	22.8655 mL	57.1638 mL
	5 mM	0.4573 mL	2.2866 mL	4.5731 mL	11.4328 mL
	10 mM	0.2287 mL	1.1433 mL	2.2866 mL	5.7164 mL
	15 mM	0.1524 mL	0.7622 mL	1.5244 mL	3.8109 mL
	20 mM	0.1143 mL	0.5716 mL	1.1433 mL	2.8582 mL
	25 mM	0.0915 mL	0.4573 mL	0.9146 mL	2.2866 mL
	30 mM	0.0762 mL	0.3811 mL	0.7622 mL	1.9055 mL
	40 mM	0.0572 mL	0.2858 mL	0.5716 mL	1.4291 mL
	50 mM	0.0457 mL	0.2287 mL	0.4573 mL	1.1433 mL
	60 mM	0.0381 mL	0.1905 mL	0.3811 mL	0.9527 mL
	80 mM	0.0286 mL	0.1429 mL	0.2858 mL	0.7145 mL
	100 mM	0.0229 mL	0.1143 mL	0.2287 mL	0.5716 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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IST5-002

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) IST5-002

All STAT Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

IST5-002

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) IST5-002

All STAT Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

IST5-002 Related Classifications

HY-19527 Related Classifications