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Cat. No.: HY-114503

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NSAH

Name: NSAH
Brand: MedChemExpress
SKU: HY-114503
Price: 80.00 USD
Availability: InStock
Rating: 5 (1 reviews)

고객리뷰

Based on 1 Customer Validation

NSAH is a reversible and competitive nonnucleoside ribonucleotide reductase (RR) inhibitor, with cell-free IC₅₀ of 32 μM and cell-based IC₅₀ of ~250 nM, respectively.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 98.45%
CAS No.: 1099592-35-4
화학식: C₁₈H₁₄N₂O₃
분자량:306.32

보관:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

NSAH Chemical Structure

CAS No.: 1099592-35-4

사이즈

가격

재고

수량

Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

HY-19931
COH29

RNR Inhibitor

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

NSAH is a reversible and competitive nonnucleoside ribonucleotide reductase (RR) inhibitor, with cell-free IC₅₀ of 32 μM and cell-based IC₅₀ of ~250 nM, respectively^[1].

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	225 nM Compound: E-3a	Growth inhibition of human HCT116 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay Growth inhibition of human HCT116 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay	[PMID: 29253340]
MDA-MB-231	IC₅₀	0.347 μM Compound: E-3a	Growth inhibition of human MDA-MB-231 cells treated with 4 pmol RRM1 siRNA after 5 days by Hoechst 33258 dye based fluorescence assay Growth inhibition of human MDA-MB-231 cells treated with 4 pmol RRM1 siRNA after 5 days by Hoechst 33258 dye based fluorescence assay	[PMID: 29253340]
MDA-MB-231	IC₅₀	1.43 μM Compound: E-3a	Growth inhibition of human MDA-MB-231 cells treated with 2 pmol RRM1 siRNA at 0.1 to 10 uM after 5 days by Hoechst 33258 dye based fluorescence assay Growth inhibition of human MDA-MB-231 cells treated with 2 pmol RRM1 siRNA at 0.1 to 10 uM after 5 days by Hoechst 33258 dye based fluorescence assay	[PMID: 29253340]
MDA-MB-231	IC₅₀	1.49 μM Compound: E-3a	Growth inhibition of human MDA-MB-231 cells after 5 days by Hoechst 33258 dye based fluorescence assay Growth inhibition of human MDA-MB-231 cells after 5 days by Hoechst 33258 dye based fluorescence assay	[PMID: 29253340]
MDA-MB-231	IC₅₀	1.94 μM Compound: E-3a	Growth inhibition of human MDA-MB-231 cells treated with 4 pmol scrambled siRNA after 5 days by Hoechst 33258 dye based fluorescence assay Growth inhibition of human MDA-MB-231 cells treated with 4 pmol scrambled siRNA after 5 days by Hoechst 33258 dye based fluorescence assay	[PMID: 29253340]
MDA-MB-231	IC₅₀	300 nM Compound: E-3a	Growth inhibition of human MDA-MB-231 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by Hoechst 33258 dye based fluorescence assay	[PMID: 29253340]

In Vitro

NSAH depresses dGTP and dATP levels in the dNTP pool causing S-phase arrest, providing evidence for RR inhibition in cells^[1].
NSAH (0-10 μM, 2, 6, 24, or 72 h) exhibits potent anti-tumor activity in 3 cancer cell lines^[1].
NSAH blocks S-phase progression well to the extent and timing of the decreases in dATP and dGTP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

MDA-231, HCT116 and Panc1 cancer cell lines.
Concentration:

0-10 μM.
Incubation Time:

2, 6, 24, or 72 h.
Result:

Resulted in IC₅₀ values ranging from 220 to 500 nM.

Chemical Information

CAS No. 1099592-35-4
Appearance Solid
분자량 306.32
화학식 C₁₈H₁₄N₂O₃
Color Light yellow to yellow
SMILES

O=C(C1=CC=CC=C1O)N/N=C/C2=C3C=CC=CC3=CC=C2O
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 62.5 mg/mL (204.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2646 mL

16.3228 mL

32.6456 mL

5 mM

0.6529 mL

3.2646 mL

6.5291 mL

10 mM

0.3265 mL

1.6323 mL

3.2646 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Md Faiz Ahmad, et al. Potent competitive inhibition of human ribonucleotide reductase by a nonnucleoside small molecule. Proc Natl Acad Sci U S A. 2017 Aug 1;114(31):8241-8246. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2646 mL	16.3228 mL	32.6456 mL	81.6140 mL
	5 mM	0.6529 mL	3.2646 mL	6.5291 mL	16.3228 mL
	10 mM	0.3265 mL	1.6323 mL	3.2646 mL	8.1614 mL
	15 mM	0.2176 mL	1.0882 mL	2.1764 mL	5.4409 mL
	20 mM	0.1632 mL	0.8161 mL	1.6323 mL	4.0807 mL
	25 mM	0.1306 mL	0.6529 mL	1.3058 mL	3.2646 mL
	30 mM	0.1088 mL	0.5441 mL	1.0882 mL	2.7205 mL
	40 mM	0.0816 mL	0.4081 mL	0.8161 mL	2.0403 mL
	50 mM	0.0653 mL	0.3265 mL	0.6529 mL	1.6323 mL
	60 mM	0.0544 mL	0.2720 mL	0.5441 mL	1.3602 mL
	80 mM	0.0408 mL	0.2040 mL	0.4081 mL	1.0202 mL
	100 mM	0.0326 mL	0.1632 mL	0.3265 mL	0.8161 mL

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MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- 제품 설명
- In Vitro
Chemical Information
용액&용해도
- In Vitro
순도&문서
References

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

NSAH

All DNA/RNA Synthesis Isoforms

Biological Activity

제품 설명

Cellular Effect

In Vitro

Chemical Information

용액&용해도

In Vitro:

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

NSAH Related Classifications

HY-114503 Related Classifications

Bioz

Keywords

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