Cat. No.: HY-150603

STAT3-IN-13

1 Cited Publications

고객리뷰

Based on 1 publication(s) in Google Scholar

STAT3-IN-13 (compound 6f) is a potent STAT3 inhibitor. STAT3-IN-13 has anti-proliferative effects and binds to the STAT3 SH2 domain with a K_D of 0.46 μM. STAT3-IN-13 inhibits the phosphorylation of STAT3 Y705 and downstream target gene expression. STAT3-IN-13 induces apoptosis in vitro and suppresses the growth and metastasis of tumor in vivo. STAT3-IN-13 can be used for cancer research.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.24%
CAS No.: 2248552-86-3
화학식: C₂₁H₂₀N₆O₃S
분자량:436.49

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

STAT3-IN-13 Chemical Structure

CAS No.: 2248552-86-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All STAT Isoforms :

All Bcl-2 Family Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
143B	IC₅₀	0.19 μM Compound: 6f	Antiinvasive activity against human 143B cells assessed as reduction in cell invasion measured after 12 hrs by crystal violet staining based inverted microscopic analysis Antiinvasive activity against human 143B cells assessed as reduction in cell invasion measured after 12 hrs by crystal violet staining based inverted microscopic analysis	[PMID: 35476936]
143B	IC₅₀	0.25 μM Compound: 6f	Antiproliferative activity against human 143B cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay Antiproliferative activity against human 143B cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay	[PMID: 35476936]
143B	IC₅₀	0.48 μM Compound: 6f	Antimigratory activity against human 143B cells assessed as reduction in cell migration measured after 12 hrs by wound healing assay Antimigratory activity against human 143B cells assessed as reduction in cell migration measured after 12 hrs by wound healing assay	[PMID: 35476936]
HOS	IC₅₀	0.11 μM Compound: 6f	Antiproliferative activity against human HOS cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay Antiproliferative activity against human HOS cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay	[PMID: 35476936]
MG-63	IC₅₀	0.55 μM Compound: 6f	Antiproliferative activity against human MG-63 cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay Antiproliferative activity against human MG-63 cells assessed as inhibition of cell growth incubated for 48 hrs by cell titer based MTS assay	[PMID: 35476936]

In Vitro

STAT3-IN-13 (compound 6f) (48 hours) has anti-proliferative activity with IC₅₀ values of 0.25, 0.11 and 0.55 μM for 143B, HOS and MG63 cells, respectively^[1].
STAT3-IN-13 (compound 6f) (0.001-100 μM) binds to STAT3 and interacts with STAT^3586−685 in a concentration dependent manner with a K_D of 0.96 μM^[1].
STAT3-IN-13 (compound 6f) (0-1.0 μM; 24 hours; 143B and HOS cells) inhibits STAT3 Y705 phosphorylation and suppresses STAT3 in tumor cells^[1].
STAT3-IN-13 (compound 6f) (0-1.0 μM; 48 hours; 143B cells) induces apoptosis in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

143B cells
Concentration:

0, 0.2, 0.5, and 1 μM
Incubation Time:

48 hours
Result:

Increased the percentage of apoptosis rates of 3.4%, 8.7%, 10.7%, and 23.3% at 0, 0.2, 0.5 and 1 μM, respectively.

Western Blot Analysis^[1]

Cell Line:

143B and HOS cells
Concentration:

0, 0.2, 0.5 and 1.0 μM
Incubation Time:

24 hours
Result:

Inhibited STAT3 Y705 phosphorylation and downstream target gene expression including Bcl-2 and VEGF, retained good antitumor activities against control tumor cells without STAT3 knockdown.

In Vivo

STAT3-IN-13 (compound 6f) (10-20 mg/kg; i.p.; twice daily, for 4 weeks; nude mice) blocks osteosarcoma growth and metastasis in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Nude mice
Dosage:

10 and 20 mg/kg
Administration:

Intraperitoneal injection; twice daily, for 4 weeks
Result:

Suppressed tumor weight at a dose of 10 mg/kg.

Chemical Information

CAS No. 2248552-86-3
Appearance Solid
분자량 436.49
화학식 C₂₁H₂₀N₆O₃S
Color Yellow to brown
SMILES

N#CC1=C(SC2=C1CCN(C2)C(NC3=CC=C(C=C3)OC)=O)NC(C(C=N4)=CN4C)=O
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

Neoplasia

GBP1-CDK9-STAT3 signaling axis promotes osteosarcoma PD-L1 expression and immune escape. [Abstract]

2025 Nov:69:101232. PMID: 40975978

용액&용해도

In Vitro:

DMSO : 125 mg/mL (286.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2910 mL

11.4550 mL

22.9100 mL

5 mM

0.4582 mL

2.2910 mL

4.5820 mL

10 mM

0.2291 mL

1.1455 mL

2.2910 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 6.25 mg/mL (14.32 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 6.25 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 6.25 mg/mL (14.32 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 6.25 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jin W, et, al. Discovery of 2-Amino-3-cyanothiophene Derivatives as Potent STAT3 Inhibitors for the Treatment of Osteosarcoma Growth and Metastasis. J Med Chem. 2022 May 12;65(9):6710-6728. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2910 mL	11.4550 mL	22.9100 mL	57.2751 mL
	5 mM	0.4582 mL	2.2910 mL	4.5820 mL	11.4550 mL
	10 mM	0.2291 mL	1.1455 mL	2.2910 mL	5.7275 mL
	15 mM	0.1527 mL	0.7637 mL	1.5273 mL	3.8183 mL
	20 mM	0.1146 mL	0.5728 mL	1.1455 mL	2.8638 mL
	25 mM	0.0916 mL	0.4582 mL	0.9164 mL	2.2910 mL
	30 mM	0.0764 mL	0.3818 mL	0.7637 mL	1.9092 mL
	40 mM	0.0573 mL	0.2864 mL	0.5728 mL	1.4319 mL
	50 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1455 mL
	60 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9546 mL
	80 mM	0.0286 mL	0.1432 mL	0.2864 mL	0.7159 mL
	100 mM	0.0229 mL	0.1146 mL	0.2291 mL	0.5728 mL

Keywords

STAT3-IN-13

1 Cited Publications

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STAT3-IN-13

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) STAT3-IN-13

All STAT Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

STAT3-IN-13

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) STAT3-IN-13

All STAT Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

STAT3-IN-13 Related Classifications

HY-150603 Related Classifications