L82-G17
Based on 1 Customer Validation
L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity.
For research use only. We do not sell to patients.
- Purity: 99.59%
- CAS No.: 92285-87-5
- Formula: C11H9ClN4O2
- Molecular Weight:264.67
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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DNA Ligase |
L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI[1].
L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding[1].
L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation[1].
L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:0-30 μM
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Incubation Time:5 days
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Result:Reduced cell number by about 70% at 20 μM.
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Cell Line:CH12F3 WT and CH12F3Δ/Δ cells
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Concentration:0-100 μM
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Incubation Time:72 h
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Result:Had great effect on the proliferation and survival of the parental CH12F3 cells.
Chemical Information
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CAS No. 92285-87-5
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Appearance Solid
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Molecular Weight 264.67
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Formula C11H9ClN4O2
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Color Pink to orange
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SMILES
O=C1NN=CC(N/N=C/C2=CC(O)=CC=C2)=C1Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (472.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.86 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (614 KB)
- English - EN (614 KB)
- Français - FR (614 KB)
- Deutsch - DE (614 KB)
- Norwegian - NO (614 KB)
- Español - ES (614 KB)
- Swedish - SV (614 KB)
- Italian - IT (614 KB)
- Korean - KR (614 KB)
- Portuguese - PT (614 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.7783 mL | 18.8914 mL | 37.7829 mL | 94.4572 mL |
| 5 mM | 0.7557 mL | 3.7783 mL | 7.5566 mL | 18.8914 mL | |
| 10 mM | 0.3778 mL | 1.8891 mL | 3.7783 mL | 9.4457 mL | |
| 15 mM | 0.2519 mL | 1.2594 mL | 2.5189 mL | 6.2971 mL | |
| 20 mM | 0.1889 mL | 0.9446 mL | 1.8891 mL | 4.7229 mL | |
| 25 mM | 0.1511 mL | 0.7557 mL | 1.5113 mL | 3.7783 mL | |
| 30 mM | 0.1259 mL | 0.6297 mL | 1.2594 mL | 3.1486 mL | |
| 40 mM | 0.0945 mL | 0.4723 mL | 0.9446 mL | 2.3614 mL | |
| 50 mM | 0.0756 mL | 0.3778 mL | 0.7557 mL | 1.8891 mL | |
| 60 mM | 0.0630 mL | 0.3149 mL | 0.6297 mL | 1.5743 mL | |
| 80 mM | 0.0472 mL | 0.2361 mL | 0.4723 mL | 1.1807 mL | |
| 100 mM | 0.0378 mL | 0.1889 mL | 0.3778 mL | 0.9446 mL |
L82-G17 Related Classifications
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