LNSMGQD 

LNSMGQD is a cyclic peptide fragment derived from desmoglein 1 (amino acids 81-86), which mimics trans-interactions and acts as part of the tandem peptide binding interface of desmoglein 2. LNSMGQD not only binds to desmoglein 1 and 3, but also effectively inhibits their homophilic trans-interactions, while reducing the probability of homophilic or heterophilic binding between desmoglein 2 and Dsc2, N-cadherin and E-cadherin. LNSMGQD is applicable to the research on disease mechanisms such as Crohn's disease and pemphigus vulgaris^[1][2][3].

The tandem peptide containing LNSMGQD (20 μM; 30 min) selectively enhances the homophilic binding of Dsg2 under cell-free conditions, and reverses the inhibitory effect of L-tryptophan without affecting E-cadherin binding^[1].
The tandem peptide containing LNSMGQD (20 μM; 24 h) prevents TNF-α-induced loss of intercellular adhesion and increase in cell permeability in CaCo2 and HCT116 intestinal epithelial cells^[1].
The tandem peptide containing LNSMGQD (20 μM; 24 h) blocks the TNF-α-induced upregulated expression of the pore-forming tight junction protein claudin-2 in intestinal epithelial CaCo2 cells, and maintains the junctional localization of Dsg2 and claudin-1^[1].
LNSMGQD (200 μM; 24 h) disrupts intercellular adhesion of human keratinocyte HaCaT cells in dispase-based dissociation assays and induces keratin filament contraction^[2].
LNSMGQD (200 μM; 30 min) induces phosphorylation of p38 MAPK in human keratinocyte HaCaT cells^[2].
LNSMGQD (40 μM; 1 h) directly interferes with the binding sites involved in homophilic and heterophilic desmoglein 2 interactions to reduce the binding probability, without altering the single-molecule unbinding force^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LNSMGQD (200 μmol/L; intradermal; single dose) increases p38 MAPK phosphorylation in the back skin of newborn Balb/c mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

763.82

C₂₉H₄₉N₉O₁₃S

1152066-26-6

Solid

White to off-white

Leu-Asn-Ser-Met-Gly-Gln-Asp

LNSMGQD

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Spindler V, et al. Loss of Desmoglein 2 Contributes to the Pathogenesis of Crohn's Disease. Inflamm Bowel Dis. 2015;21(10):2349-2359.  [Content Brief]

  [2]. Spindler V, et al. Peptide-mediated desmoglein 3 crosslinking prevents pemphigus vulgaris autoantibody-induced skin blistering. J Clin Invest. 2013;123(2):800-811.  [Content Brief]

  [3]. Fuchs M, et al. Desmoglein 2 can undergo Ca2+-dependent interactions with both desmosomal and classical cadherins including E-cadherin and N-cadherin[J]. Biophysical journal, 2022, 121(7): 1322-1335.