Malabaricone C
Based on 3 publication(s) in Google Scholar
Malabaricone C is an orally active and noncompetitive sphingomyelin synthase (SMS) inhibitor with IC50 values of 3 μM and 1.5 μM for SMS 1 and SMS 2, respectively. Malabaricone C reduces body weight gain, improves glucose tolerance, and decreases lipid accumulation in the liver, showing significant prevention of high fat diet-induced fatty liver in mice. Malabaricone C has anti-inflammatory effects, which is found in the fruits of Myristica cinnamomea King. Malabaricone C is promising for research of obesity and immunological disorders caused due to hyper-activation of T-cells.
For research use only. We do not sell to patients.
- Purity : 99.46%
- CAS No.: 63335-25-1
- Formula: C21H26O5
- Molecular Weight:358.43
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-N6719
Fumonisin B1Sphingosine N-acyltransferase Inhibitor -
HY-114307
Ly93SMS2 Inhibitor -
HY-N6798
MyriocinSPT Inhibitor -
HY-10038
A 922500DGAT-1 Inhibitor -
HY-13966
2-Deoxy-D-glucoseGlycolysis Inhibitor -
HY-13992
AP20187FKBP Dimerizer -
HY-160818
MC3138SIRT5 Inhibitor -
HY-102041
SMS2-IN-1SMS2 Inhibitor -
HY-117632
ElcubragistatMAGL Inhibitor -
HY-171160
WYFA-15SMS1 Inhibitor -
HY-N8518R
Malabaricone C (Standard)Sphingomyelin Synthase Inhibitor -
HY-B0150S
Nicotinamide-d4Stable Isotope
Publications Citing Use of MedChemExpress (MCE) Malabaricone C
More
Customer Validation & Images
-
WB
-
Bio/Physico-chemical Assay
-
Cell Imaging/Staining
-
Apoptosis Analysis
-
Cell Proliferation/Viability Assay
All Phospholipase Isoforms
More
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
5.26 μM
Compound: Mal C
|
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
|
[PMID: 20805034] |
| MEF | IC50 |
11 μM
Compound: 3
|
Inhibition of SMS2 in MEF cells using C6-NBD-ceramide as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by HPLC analysis
Inhibition of SMS2 in MEF cells using C6-NBD-ceramide as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by HPLC analysis
|
[PMID: 31413799] |
| MEF | IC50 |
13 μM
Compound: 3
|
Inhibition of SMS1 in MEF cells using C6-NBD-ceramide as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by HPLC analysis
Inhibition of SMS1 in MEF cells using C6-NBD-ceramide as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by HPLC analysis
|
[PMID: 31413799] |
| RAW264.7 | IC50 |
2.3 μM
Compound: 5, Malabaricone C
|
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess reagent method
|
[PMID: 21978676] |
In Vitro
Malabaricone C (0.01-1 mM, 3 h) has little cytotoxic activity against wild-type mouse embryonic fibroblasts cells, MEF[1].
Malabaricone C (0.01-1 mM, 3 h) decreases the levels of intracellular triglycerides and decreases lipid accumulation in HepG2 cells[1].
Malabaricone C (1-10 μM, 2 h) inhibits T-cell activation, proliferation, and cytokine production[2].
Malabaricone C (10 μM, 1 h or 24 h) suppresses mitogen-induced activation of cell surface markers, MAPK and NF-κB in lymphocytes[2].
Malabaricone C (4-10 μ M, 24 h) induces apoptosis in MCF-7 cells[3].
Malabaricone C (0-6 μ M, 9 min) increases intracellular Ca2+ levels and activates calpain in MCF-7 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
-
Cell Line:Mouse embryonic fibroblasts cells
-
Concentration:0.01-1 mM
-
Incubation Time:3 h
-
Result:Induced 56−97% of the cells viable after 3 h of treatment at concentration levels of 1-0.01 mM in MEFs.
-
Cell Line:HepG2 cells
-
Concentration:0.01-1 mM
-
Incubation Time:3 h
-
Result:Significantly decreased the levels of intracellular triglycerides in a dose-dependent manner while it exhibited no significant changes in the free fatty acids levels, decreased lipid accumulation in a dose-dependent manner.
-
Cell Line:Lymphocytes
-
Concentration:1-10 μM
-
Incubation Time:2 h
-
Result:Inhibited the proliferation of αCD3/ αCD28-activated T-cells and suppressed the secretion of IL-2 and IFN-γ.
-
Cell Line:MCF-7 cells
-
Concentration:4-10 μM
-
Incubation Time:24 h
-
Result:Showed condensed nuclei and showed significantly increased (up to 64%, p < 0.001) sub G1 population in MCF-7 cells.
In Vivo
Malabaricone C (10 mg/kg, i.p., a single dose for 24 h) inhibits T-cell activation and cytokine secretion in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:High fat diet-induced obesity mice models[1]
-
Dosage:High-fat diet supplemented with 0.1%
-
Administration:p.o., daily for 8 weeks
-
Result:Reduced weight gain, improved glucose tolerance, and reduced hepatic steatosis in high fat diet-induced obesity mice models.
-
Animal Model:Swiss, BALB/c, and C57BL/6 male mice[2]
-
Dosage:10 mg/kg
-
Administration:i.p., a single dose for 24 h
-
Result:Significantly decreased the percentage of proliferating cells as compared with vehicle control and resulted in significant decrease in mitogen-induced cytokine (IL-2, IFN-γ, and IL-6) secretion by T-cells.
Chemical Information
-
CAS No. 63335-25-1
-
Appearance Solid
-
Molecular Weight 358.43
-
Formula C21H26O5
-
Color White to light yellow
-
SMILES
O=C(C1=C(O)C=CC=C1O)CCCCCCCCC2=CC=C(O)C(O)=C2
-
Structure Classification
-
Initial Source
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
-
Journal Impact Factor
-
Most Recent
-
Adv Sci (Weinh)
2025 Jul 28:e15311. PMID: 40719020
Malabaricone C purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 28:e15311. [Abstract]
Malabaricone C (10 μM) efficiently suppressed SGMS1 and NAMPT protein expression in C2C12 cells.
Malabaricone C purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 28:e15311. [Abstract]
Malabaricone C (10 μM) efficiently decreased NAD+ levels in C2C12 cells exposed to H2O2-induced oxidative stress.
-
Oncogene
TMSB4Y restrains sphingomyelin synthesis via de novo purine synthesis to exert a tumor suppressor function in male esophageal squamous cell carcinoma. [Abstract]2024 Dec;43(50):3660-3672. PMID: 39443723
Malabaricone C purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Dec;43(50):3660-3672. [Abstract]
Malabaricone C (Ma C; 20, 40 μM; 1, 2, 3, 4 days) suppressed cell proliferation of KYSE30 and KYSE410 cells.
Malabaricone C purchased from MedChemExpress. Usage Cited in: Oncogene. 2024 Dec;43(50):3660-3672. [Abstract]
Malabaricone C (Ma C; 10 mg/kg; p.o.; daily for 7 days) inhibited xenograft tumor growth in 6-week-old male BALB/c-nu/nu mice (KYSE30 cells).
-
Malabaricone C purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Aug 13.
Malabaricone C (10 μM; 30 min) restored TRPML1 channel activity in HCT116 cells treated with V8 (12 μM).
Malabaricone C purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Aug 13.
Malabaricone C (10 μM; 12 h) attenuated V8-induced apoptosis in HCT116 and Hela cells.
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (278.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
-
3T3-L1 preadipocyte-to-adipocyte differentiation
3T3-L1 preadipocytes are induced to differentiate after growth arrest using adipogenic media containing insulin, dexamethasone, and IBMX; differentiation is assessed by lipid-droplet accumulation, triglyceride increase, Oil Red O staining, and adipocyte-marker induction such as PPARγ and C/EBPα.
-
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
-
Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
-
Research Protocol for Metabolic Diseases
AMP-activated protein kinase, AMPK, is a conserved cellular energy sensor that responds to reduced cellular energy status and coordinates metabolism by increasing ATP-generating catabolic pathways while suppressing ATP-consuming anabolic processes. In metabolic disease research, the AMPK pathway is experimentally relevant because it regulates hepatic lipid synthesis, fatty acid oxidation, glucose production, skeletal-muscle glucose disposal, mTORC1-linked biosynthesis, autophagy, mitochondrial homeostasis, and whole-body energy balance. The central pathway logic is that energy stress, metformin, exercise-like stimulation, or direct AMPK activators increase AMPKα Thr172 phosphorylation and downstream substrate phosphorylation, including ACC and RAPTOR. Phosphorylation of ACC suppresses lipogenesis and supports fatty acid oxidation, whereas phosphorylation of RAPTOR suppresses mTORC1 signaling and links cellular energy status to growth and protein synthesis control. The pathway is linked
-
Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Othman MA, et al. Malabaricone C as Natural Sphingomyelin Synthase Inhibitor against Diet-Induced Obesity and Its Lipid Metabolism in Mice. ACS Med Chem Lett. 2019;10(8):1154-1158. Published 2019 Jul 3. [Content Brief]
[2]. Patwardhan RS, et al. Malabaricone C, a constituent of spice Myristica malabarica, exhibits anti-inflammatory effects via modulation of cellular redox. J Biosci. 2023;48(2):9. [Content Brief]
[3]. Tyagi M, et al. Mechanism of the malabaricone C-induced toxicity to the MCF-7 cell line. Free Radic Res. 2014 Apr;48(4):466-77. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7899 mL | 13.9497 mL | 27.8995 mL | 69.7486 mL |
| 5 mM | 0.5580 mL | 2.7899 mL | 5.5799 mL | 13.9497 mL | |
| 10 mM | 0.2790 mL | 1.3950 mL | 2.7899 mL | 6.9749 mL | |
| 15 mM | 0.1860 mL | 0.9300 mL | 1.8600 mL | 4.6499 mL | |
| 20 mM | 0.1395 mL | 0.6975 mL | 1.3950 mL | 3.4874 mL | |
| 25 mM | 0.1116 mL | 0.5580 mL | 1.1160 mL | 2.7899 mL | |
| 30 mM | 0.0930 mL | 0.4650 mL | 0.9300 mL | 2.3250 mL | |
| 40 mM | 0.0697 mL | 0.3487 mL | 0.6975 mL | 1.7437 mL | |
| 50 mM | 0.0558 mL | 0.2790 mL | 0.5580 mL | 1.3950 mL | |
| 60 mM | 0.0465 mL | 0.2325 mL | 0.4650 mL | 1.1625 mL | |
| 80 mM | 0.0349 mL | 0.1744 mL | 0.3487 mL | 0.8719 mL | |
| 100 mM | 0.0279 mL | 0.1395 mL | 0.2790 mL | 0.6975 mL |
Malabaricone C Related Classifications
HY-N8518 Related Classifications
Powered by Bioz