Maleic hydrazide
Based on 1 Customer Validation
Maleic hydrazide is extensively used as a systemic plant growth regulator and as a herbicide. Maleic hydrazide acts as an inhibitor of the synthesis of nucleic acids and proteins.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 123-33-1
- Formula: C4H4N2O2
- Molecular Weight:112.09
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Maleic hydrazide is used in agriculture-in despite its known effect as a mutagenic and clastogenic agent. Maleic hydrazide had lower IC50 values for all cell lines compared to Ethephon. Maleic hydrazide also showed least cytotoxicity on Vero cells, followed by Hep2 and HepG2 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 123-33-1
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Appearance Solid
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Molecular Weight 112.09
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Formula C4H4N2O2
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Color White to off-white
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SMILES
O=C1C=CC(NN1)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (1115.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (18.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (18.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (618 KB)
- English - EN (618 KB)
- Français - FR (618 KB)
- Deutsch - DE (618 KB)
- Norwegian - NO (618 KB)
- Español - ES (618 KB)
- Swedish - SV (618 KB)
- Italian - IT (618 KB)
- Korean - KR (618 KB)
- Portuguese - PT (618 KB)
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Handling Instructions (2659 KB)
References
[1]. Venezian A, et al. The Influence of the Plant Growth Regulator Maleic Hydrazide on Egyptian Broomrape Early Developmental Stages and Its Control Efficacy in Tomato under Greenhouse and Field Conditions. Front Plant Sci. 2017;8:691. Published 2017 May 16. [Content Brief]
[2]. Swietlińska Z, et al. Cytotoxic effects of maleic hydrazide. Mutat Res. 1978;55(1):15-30. [Content Brief]
[3]. Yurdakok B, et al. Cytotoxic effects of etephon and maleic hydrazide in Vero, Hep2, HepG2 cells. Drug Chem Toxicol. 2014;37(4):459-465. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.9214 mL | 44.6070 mL | 89.2140 mL | 223.0351 mL |
| 5 mM | 1.7843 mL | 8.9214 mL | 17.8428 mL | 44.6070 mL | |
| 10 mM | 0.8921 mL | 4.4607 mL | 8.9214 mL | 22.3035 mL | |
| 15 mM | 0.5948 mL | 2.9738 mL | 5.9476 mL | 14.8690 mL | |
| 20 mM | 0.4461 mL | 2.2304 mL | 4.4607 mL | 11.1518 mL | |
| 25 mM | 0.3569 mL | 1.7843 mL | 3.5686 mL | 8.9214 mL | |
| 30 mM | 0.2974 mL | 1.4869 mL | 2.9738 mL | 7.4345 mL | |
| 40 mM | 0.2230 mL | 1.1152 mL | 2.2304 mL | 5.5759 mL | |
| 50 mM | 0.1784 mL | 0.8921 mL | 1.7843 mL | 4.4607 mL | |
| 60 mM | 0.1487 mL | 0.7435 mL | 1.4869 mL | 3.7173 mL | |
| 80 mM | 0.1115 mL | 0.5576 mL | 1.1152 mL | 2.7879 mL | |
| 100 mM | 0.0892 mL | 0.4461 mL | 0.8921 mL | 2.2304 mL |
Maleic hydrazide Related Classifications
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