Multi-kinase inhibitor 3
Multi-kinase inhibitor 3 (compound 12) is a potent and orally active multikinase inhibitor with IC50 values of 1.59, 1.23, 1.19, 0.59, 0.22, 1.15 nM for FLT1/VEGFR1, KDR/VEGFR2, FLT4/VEGFR3, FLT3, PDGFRα, PDGFRβ, respectively. Multi-kinase inhibitor 3 shows antiproliferative activity. Multi-kinase inhibitor 3 shows anticancer activity.
For research use only. We do not sell to patients.
- CAS No.: 2648279-76-7
- Formula: C26H26N6O2
- Molecular Weight:454.52
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All VEGFR Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
VEGFR1 1.59 nM (IC50) |
VEGFR2 1.23 nM (IC50) |
VEGFR3 1.19 nM (IC50) |
PDGFRα 0.22 nM (IC50) |
PDGFR-β 1.15 nM (IC50) |
Chemical Information
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CAS No. 2648279-76-7
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Molecular Weight 454.52
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Formula C26H26N6O2
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SMILES
CN1N=CC(C2=CC=C(N(C3=CC=C(CC(NC4=CC(C(C)(C)C)=NO4)=O)C=C3)C=N5)C5=C2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Multi-kinase inhibitor 3 Related Classifications
HY-168114 Related Classifications
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