N6-Methyladenine
Based on 1 Customer Validation
N6-Methyladenine is a DNA epigenetic modification that involves the addition of a methyl group to the sixth position of adenine. N6-Methyladenine plays an important role in distinguishing host DNA from exogenous DNA and controls many biological functions, such as DNA replication, transcription, mismatch repair, and chromosome replication. N6-Methyladenine can be used for the kidney diseases.
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- Purity : 98.79%
- CAS No.: 443-72-1
- 화학식: C6H7N5
- 분자량:149.16
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Adenine hemisulfate (Standard)Nucleobase -
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Adenine (Standard)Nucleobase -
HY-B0617AR
S-Adenosyl-L-methionine tosylate (Standard)Standard of S-Adenosyl-L-methionine tosylate -
HY-B1449R
Uridine (Standard)Nucleoside
Biological Activity
제품 설명
In Vitro
N6-methyladenine is the most common internal epigenetic modification in eukaryotic mRNA and regulates its dynamic and reversible process through three classes of enzymes: methyltransferases (writers), demethylases (erasers), and methylation recognition proteins (readers). N6-methyladenine plays a central role in key biological processes such as RNA splicing, stability, translocation, and translation. N6-methyladenine is involved in physiological processes such as nervous system development. N6-methyladenine affects the pathogenesis of acute kidney injury (AKI, such as ischemia-reperfusion-induced AKI, cisplatin-induced AKI), acute kidney disease (AKD), and chronic kidney disease (CKD, such as diabetic kidney disease, lupus nephritis, autosomal dominant polycystic kidney disease) by regulating target genes (e.g., Foxd1, YAP1, TAB3, Sirt1) and related signaling pathways (e.g., PI3K/Akt, ERK/NF-κB, NLRP3 inflammasome)[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 443-72-1
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Appearance Solid
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분자량 149.16
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화학식 C6H7N5
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Color White to light brown
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SMILES
CNC1=C2NC=NC2=NC=N1
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
DMSO : 20 mg/mL (134.08 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 2 mg/mL (13.41 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (13.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (13.41 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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PCNA Immunodetection Proliferation Assay
PCNA immunodetection measures proliferative activity by detecting proliferating cell nuclear antigen, a nuclear protein associated with DNA polymerase δ function and DNA replication. The assay readout is the proportion of PCNA-positive nuclei among total counted cells, but PCNA labeling is not identical to BrdU labeling because PCNA can mark late G1/early S-associated replication competence and may persist beyond active DNA synthesis depending on fixation and extraction conditions.
순도&문서
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Data Sheet (278 KB)
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SDS (477 KB)
- English - EN (477 KB)
- Français - FR (477 KB)
- Deutsch - DE (477 KB)
- Norwegian - NO (477 KB)
- Español - ES (477 KB)
- Swedish - SV (477 KB)
- Italian - IT (477 KB)
- Korean - KR (477 KB)
- Portuguese - PT (477 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 6.7042 mL | 33.5211 mL | 67.0421 mL | 167.6053 mL |
| 5 mM | 1.3408 mL | 6.7042 mL | 13.4084 mL | 33.5211 mL | |
| 10 mM | 0.6704 mL | 3.3521 mL | 6.7042 mL | 16.7605 mL | |
| DMSO | 15 mM | 0.4469 mL | 2.2347 mL | 4.4695 mL | 11.1737 mL |
| 20 mM | 0.3352 mL | 1.6761 mL | 3.3521 mL | 8.3803 mL | |
| 25 mM | 0.2682 mL | 1.3408 mL | 2.6817 mL | 6.7042 mL | |
| 30 mM | 0.2235 mL | 1.1174 mL | 2.2347 mL | 5.5868 mL | |
| 40 mM | 0.1676 mL | 0.8380 mL | 1.6761 mL | 4.1901 mL | |
| 50 mM | 0.1341 mL | 0.6704 mL | 1.3408 mL | 3.3521 mL | |
| 60 mM | 0.1117 mL | 0.5587 mL | 1.1174 mL | 2.7934 mL | |
| 80 mM | 0.0838 mL | 0.4190 mL | 0.8380 mL | 2.0951 mL | |
| 100 mM | 0.0670 mL | 0.3352 mL | 0.6704 mL | 1.6761 mL |
N6-Methyladenine Related Classifications
HY-116887 Related Classifications
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