NADPH

Name: NADPH
Brand: MedChemExpress
SKU: HY-113324
Rating: 5.0 (18 reviews)

Cat. No.: HY-113324 Purity: 99.75%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

NADPH is a coenzyme of glutathione reductase (GR), thioredoxin reductase (TrxR) and NADPH oxidase (NOX), and participates in redox reactions as a hydrogen donor. NADPH has the characteristic of selectively participating in the regulation of cellular redox homeostasis. NADPH exerts antioxidant activity and resists reactive oxygen species (ROS) damage by providing reducing equivalents for the regeneration of glutathione (GSH) and thioredoxin (Trx); at the same time, it acts as a substrate of NOX to generate superoxide anions, mediating oxidative stress and immune response. NADPH participates in maintaining the intracellular reducing environment, biosynthesis and regulating gene expression (such as the Nrf2 pathway), and is mainly used in the study of oxidative stress-related diseases (such as cardiovascular diseases, neurodegenerative diseases, cancer) and immune regulation mechanisms.

For research use only. We do not sell to patients.

CAS No. : 53-57-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 18 publication(s) in Google Scholar

Other Forms of NADPH:

NADPH tetrasodium salt In-stock
NADPH tetracyclohexanamine In-stock

18 Publications Citing Use of MCE NADPH

Cell Imaging/Staining

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: NADPH purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e07283. [Abstract]; A normal cellular state was recovered by adding TEMPO or NADPH (1mM, 24h), or SLC7A11 knockdown.

: NADPH purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e07283. [Abstract]; Western Blot analysis of RAC/WAVE pathway-related indicators in vitro skeletal muscle cells. Muscle cells treated with different condition NADPH(1mM, 24h).

: NADPH purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e07283. [Abstract]; mtROS fluorescence staining of skeletal muscle cells in vitro. Muscle cells treated with different condition NADPH(1mM, 24h).

: NADPH purchased from MedChemExpress. Usage Cited in: Mol Plant Pathol. 2025 Dec;26(12):e70196. [Abstract]; Enzymatic activities of AtCAD7, AtCAD5, EcYahK, and EcYjgB toward (Z)‐3‐hexenal, acrolein, isovaleraldehyde, salicylaldehyde, and p‐coumaryl aldehyde. No Substrate was negative control. Assays were conducted under standardised conditions (30°C, pH 6.5, 100 μM substrate, 200 μM NADPH).

: NADPH purchased from MedChemExpress. Usage Cited in: Mol Ther Oncol. 2024 Sep 17;32(4):200878. [Abstract]; Apoptosis proportion of oncoVV-AVL-treated AsPC-1(Up) and PANC-1 (Down) cells treated with NADPH (300 μM) or NADH. Data are presented as the mean ± SD (n=3).

: NADPH purchased from MedChemExpress. Usage Cited in: Mol Ther Oncol. 2024 Sep 17;32(4):200878. [Abstract]; Western blotting detection of the expressions of caspase-3 and caspase-9 in oncoVV or oncoVV-AVL-treated AsPC-1(Up) and PANC-1 (Down) cells treated with NADPH (300 μM).

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

In NADK-overexpressing cells, a 4-5-fold increase in NADK concentration increases the survival rate of HEK293 cells after treatment with 1 mM H₂O₂ and 0.1 mM Menadione (HY-B0332) (treatment for 24 h)^[2].
The mRNA and protein expressions of PRDX5 and Nrf2 are also decreased in NADK-overexpressing HEK293 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HEK293 cells (control, NADK overexpressing NADK⁺, NADK knockdown NADK^-)
Concentration:	Intracellular NADPH increased 4-5-fold in NADK(+) cells
Incubation Time:
Result:	Decreased by ~70% in NADK^- cells (compared to control) 30 min treatment with 1 mM H2O2 or 100 μM Menadione, followed by 24 h recovery Showed significantly higher viability (10-20% increase) than control cells after H₂O₂ or Menadione treatment, while NADK^- cells exhibited a slight decrease (10%) in viability compared to contro.

Molecular Weight

745.42

Formula

C₂₁H₃₀N₇O₁₇P₃

CAS No.

53-57-6

Appearance

Solid

Color

White to light yellow

SMILES

O=P(O)(O)O[C@H]1[C@H](N2C3=NC=NC(N)=C3N=C2)O[C@@H]([C@H]1O)COP(OP(OC[C@H]4O[C@@H](N5C=C(CC=C5)C(N)=O)[C@@H]([C@@H]4O)O)(O)=O)(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 116.67 mg/mL (156.52 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3415 mL	6.7076 mL	13.4153 mL
5 mM	0.2683 mL	1.3415 mL	2.6831 mL
10 mM	0.1342 mL	0.6708 mL	1.3415 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (284 KB) SDS (251 KB)

COA (263 KB) HNMR (338 KB) CNMR (234 KB) RP-HPLC (257 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Xia W, et al. Roles of NAD (+)/NADH and NADP (+)/NADPH in cell death. Curr Pharm Des. 2009;15 (1):12-9. [Content Brief]

[2]. Pollak N, et al. NAD kinase levels control the NADPH concentration in human cells. J Biol Chem. 2007 Nov 16;282 (46):33562-33571. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.3415 mL	6.7076 mL	13.4153 mL	33.5381 mL
	5 mM	0.2683 mL	1.3415 mL	2.6831 mL	6.7076 mL
	10 mM	0.1342 mL	0.6708 mL	1.3415 mL	3.3538 mL
	15 mM	0.0894 mL	0.4472 mL	0.8944 mL	2.2359 mL
	20 mM	0.0671 mL	0.3354 mL	0.6708 mL	1.6769 mL
	25 mM	0.0537 mL	0.2683 mL	0.5366 mL	1.3415 mL
	30 mM	0.0447 mL	0.2236 mL	0.4472 mL	1.1179 mL
	40 mM	0.0335 mL	0.1677 mL	0.3354 mL	0.8385 mL
	50 mM	0.0268 mL	0.1342 mL	0.2683 mL	0.6708 mL
	60 mM	0.0224 mL	0.1118 mL	0.2236 mL	0.5590 mL
	80 mM	0.0168 mL	0.0838 mL	0.1677 mL	0.4192 mL
	100 mM	0.0134 mL	0.0671 mL	0.1342 mL	0.3354 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

NADPH Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NADPH53-57-6Endogenous Metabolitereducing agentelectrosynthesisInhibitorinhibitorinhibit

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NADPH

Customer Review

Other Forms of NADPH:

NADPH Related Antibodies

18 Publications Citing Use of MCE NADPH

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•Related Screening Libraries:

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View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

NADPH Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

NADPH Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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