NC1
Based on 1 Customer Validation
NC1 is a selective non-competitive and allosteric lymphoid-specific tyrosine phosphatase (LYP) inhibitor, with a Ki value 4.3 μM. NC1 allosterically regulates LYP/PTPN22 activity by restricting WPD loop movement. NC1 inhibits LYP activity in lymphoid T cells and enhances T-cell receptor signaling. NC1 can be used for the research of autoimmune diseases.
For research use only. We do not sell to patients.
- Purity: 98.73%
- CAS No.: 445406-82-6
- Formula: C29H26N2O7S
- Molecular Weight:546.59
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
NC1 (0-10 μM) non-competitively inhibits wild-type LYP with a Ki of 4.3 ± 0.3 μM in a pNPP hydrolysis biochemical assay[1].
NC1 shows reduced binding affinity for LYP mutants with alterations to residues T36, R266, H196, F28, D197, and C129, indicating these residues contribute to NC1's interaction with LYP[1].
NC1 (20 μM; 45 min pre-incubation) effectively and specifically enhances TCR signaling in Jurkat T cells by inhibiting endogenous LYP activity, as demonstrated by increased LCK and ERK phosphorylation that is abolished by LYP knockdown[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Jurkat T cells (human lymphoid T cell line)
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Concentration:20 μM
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Incubation Time:45 min (pre-incubation)
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Result:Significantly augmented anti-CD3-induced phosphorylation of LCK (at Y394) and ERK (at T202/Y204) in control siRNA-treated Jurkat T cells.
Showed no effect on anti-CD3-induced phosphorylation of LCK or ERK in LYP siRNA-treated Jurkat T cells.
Chemical Information
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CAS No. 445406-82-6
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Appearance Solid
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Molecular Weight 546.59
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Formula C29H26N2O7S
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Color Light yellow to yellow
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SMILES
O=C(C1=CC=C(COC2=CC=C(/C=C3S/C(N(C)C/3=O)=N/C4=CC=C(C(OCC)=O)C=C4)C=C2OC)C=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (182.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8295 mL | 9.1476 mL | 18.2952 mL | 45.7381 mL |
| 5 mM | 0.3659 mL | 1.8295 mL | 3.6590 mL | 9.1476 mL | |
| 10 mM | 0.1830 mL | 0.9148 mL | 1.8295 mL | 4.5738 mL | |
| 15 mM | 0.1220 mL | 0.6098 mL | 1.2197 mL | 3.0492 mL | |
| 20 mM | 0.0915 mL | 0.4574 mL | 0.9148 mL | 2.2869 mL | |
| 25 mM | 0.0732 mL | 0.3659 mL | 0.7318 mL | 1.8295 mL | |
| 30 mM | 0.0610 mL | 0.3049 mL | 0.6098 mL | 1.5246 mL | |
| 40 mM | 0.0457 mL | 0.2287 mL | 0.4574 mL | 1.1435 mL | |
| 50 mM | 0.0366 mL | 0.1830 mL | 0.3659 mL | 0.9148 mL | |
| 60 mM | 0.0305 mL | 0.1525 mL | 0.3049 mL | 0.7623 mL | |
| 80 mM | 0.0229 mL | 0.1143 mL | 0.2287 mL | 0.5717 mL | |
| 100 mM | 0.0183 mL | 0.0915 mL | 0.1830 mL | 0.4574 mL |
NC1 Related Classifications
HY-156659 Related Classifications
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