1. Metabolic Enzyme/Protease
    Anti-infection
    Cell Cycle/DNA Damage
  2. Endogenous Metabolite
    Enterovirus
    HCV
    Topoisomerase
    SARS-CoV
  3. NHC-diphosphate triammonium

NHC-diphosphate triammonium 

Cat. No.: HY-135867F Purity: 98.80%
Handling Instructions

NHC-triphosphate triammonium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form. NHC-triphosphate triammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA.

For research use only. We do not sell to patients.

NHC-diphosphate triammonium Chemical Structure

NHC-diphosphate triammonium Chemical Structure

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1 mg USD 380 In-stock
Estimated Time of Arrival: December 31
5 mg USD 800 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

NHC-triphosphate triammonium is an active phosphorylated intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) (HY-125033) as a triphosphate form[1]. NHC-triphosphate triammonium is a weak alternative substrate for the viral polymerase and can be incorporated into HCV replicon RNA[1][2].

In Vitro

In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1].
NHC-triphosphate triammonium (NHC-TP) (5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1].
Huh-7 cells are incubated with (10-50 μM; 4 h) NHC or a McGuigan phosphoramidate prodrug of NHC. Intracellular levels of the parental compounds and phosphorylated metabolites are measured using LC-MS/MS. Small amounts of NHC-monophosphate (MP) and NHC-diphosphate (DP) can be observed, while NHC-triphosphate triammonium (HY-135867) remains the most abundant metabolite[2].
NHC-triphosphate triammonium (NHC-TP) metabolite may directly target the viral polymerase and behave as a nonobligate chain terminator. It plays a prominent role in inhibiting early negative-strand RNA synthesis, either through chain termination or mutagenesis, which may in turn interfere with correct replicase complex formation.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

470.27

Formula

C₉H₂₄N₆O₁₂P₂

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 160 mg/mL (340.23 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1264 mL 10.6322 mL 21.2644 mL
5 mM 0.4253 mL 2.1264 mL 4.2529 mL
10 mM 0.2126 mL 1.0632 mL 2.1264 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Product Name:
NHC-diphosphate triammonium
Cat. No.:
HY-135867F
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