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Propidium Iodide

160 Cited Publications
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Based on 160 publication(s) in Google Scholar

Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

For research use only. We do not sell to patients.

  • Purity: 99.77%
  • CAS No.: 25535-16-4
  • Formula: C27H34I2N4
  • Molecular Weight:668.39
  • Storage:

    4°C, sealed storage, away from moisture and light

    * In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

  • Propidium Iodide Chemical Structure
    Propidium Iodide Chemical Structure
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
  • Experimental Validation Results for Propidium Iodide
CAS No.: 25535-16-4
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  • Propidium Iodide Chemical Structure
    Propidium Iodide Chemical Structure
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    Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 28;17(4):3334-3345. [Abstract]

    Customer Validation
     Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Apr 5;920:174830. [Abstract]

    Customer Validation
     RCMs were stained with Propidium Iodide (PI, 2 μg/mL; 2 h) and analyzed using a Nikon Ti2 microscope.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Oct 9;27(19):6733. [Abstract]

    Customer Validation
     A549 cells were treated with erdafitinib (0, 6.25 and 12.5 μM, 24 h) and cell apoptosis was analyzed by flow cytometry and statistically analyzed. The Annexin V+/PI- and Annexin V+/PI+ cells were considered as early and late apoptotic cells, respectively, and the sum of the above two was calculated as apoptotic cells. Propidium iodide (PI, 50 μg/mL) was added for incubation for 30 min in the dark.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2022 May;13(9):1299-1310. [Abstract]

    Customer Validation
     KYSE450 and ECA109 cells after 96 h various transfections were washed three times in cold PBS, stained with Propidium iodide (PI, 50 μg/mL) and Annexin V‐FITC (25 μg/mL) for 20 min in the dark, and analyzed within 1 h.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]

    Customer Validation
     PAMs cells were washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei were counterstained with propidium iodide (red). Fluorescent images were acquired with a confocal laser scanning microscope.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]

    Customer Validation
     PK-15CD163 cells were washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei were counterstained with propidium iodide (red). Fluorescent images were acquired with a confocal laser scanning microscope.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133. [Abstract]

    Customer Validation
     The NP tissue slices are incubated with a culture medium containing 1 μg/ml Calcein AM and 1 μg/ml PI for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.

  • 1/16

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370. [Abstract]

    Customer Validation
     KLE cells were stained by 25 μg/mL Propidium Iodide (PI) in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis was determined by Annexin V/PI double staining and percentages of cells in each phase (LL, viable; LR, early apoptotic; UL and UR, late apoptotic/necrotic cell) were calculated.

  • Propidium Iodide