2. PI3K/Akt/mTOR PROTAC Autophagy
  3. mTOR PROTACs Autophagy
  4. PD-M6

PD-M6 is a mTOR PROTAC degrader (DC50: 4.8 μM). PD-M6 promotes ubiquitination and degradation of mTOR. PD-M6 downregulates MAPKAP1 and CASTOR1, and induces Autophagy. PD-M6 inhibits the proliferation of cervical cancer, breast cancer and liver cancer cell lines. PD-M6 can be used for the research of cervical cancer, breast cancer and liver cancer.
(Pink: mTOR ligand (HY-B0795); Blue: Cereblon ligand (HY-41547); Black: linker (HY-W008296)).

For research use only. We do not sell to patients.

PD-M6

PD-M6 Chemical Structure

CAS No. : 3058330-82-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

PD-M6 Related Antibodies

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

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Description

PD-M6 is a mTOR PROTAC degrader (DC50: 4.8 μM). PD-M6 promotes ubiquitination and degradation of mTOR. PD-M6 downregulates MAPKAP1 and CASTOR1, and induces Autophagy. PD-M6 inhibits the proliferation of cervical cancer, breast cancer and liver cancer cell lines. PD-M6 can be used for the research of cervical cancer, breast cancer and liver cancer[1]. (Pink: mTOR ligand (HY-B0795); Blue: Cereblon ligand (HY-41547); Black: linker (HY-W008296)).

In Vitro

PD-M6 (0.001-10 μM; 4-24 h) induces dose- and time-dependent degradation of mTOR in HeLa cells, with a DC50 of 4.8 μM[1].
PD-M6 inhibits the proliferation of HeLa, MCF-7 and HepG2 cancer cells, with IC50 values of 11.3 μM, 2.58 μM and 3.23 μM, respectively[1].
PD-M6 (0.1-10 μM; 4 days) inhibits long-term colony formation of HeLa cells[1].
PD-M6 (10 μM) inhibits the migration of HeLa cells with an inhibition rate of over 50%[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PD-M6 Related Antibodies

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 0.001-10 μM
Incubation Time: 4 h, 12 h, 24 h
Result: Induced a dose-dependent reduction in mTOR levels, with significant degradation observed at 1 μM and maximum degradation (~90%) at 10 μM after 12 h.
Achieved a DC50 for mTOR degradation of 4.8 μM.
Reduced mTOR levels by ~50% at 4 h and ~90% at 24 h at 10 μM.

Cell Proliferation Assay[1]

Cell Line: HeLa cells
Concentration: 0.1-10 μM
Incubation Time: 4 days
Result: Reduced HeLa cell colony formation in a concentration-dependent manner, with nearly 90% suppression of proliferation observed at 10 μM.
Molecular Weight

621.69

Formula

C30H39N9O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1N(C2CCC(NC2=O)=O)C(C3=CC=CC(NCCCCCCNC4=NC(N5CCOCC5)=NC(N6CCOCC6)=N4)=C13)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (160.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6085 mL 8.0426 mL 16.0852 mL
5 mM 0.3217 mL 1.6085 mL 3.2170 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6085 mL 8.0426 mL 16.0852 mL 40.2130 mL
5 mM 0.3217 mL 1.6085 mL 3.2170 mL 8.0426 mL
10 mM 0.1609 mL 0.8043 mL 1.6085 mL 4.0213 mL
15 mM 0.1072 mL 0.5362 mL 1.0723 mL 2.6809 mL
20 mM 0.0804 mL 0.4021 mL 0.8043 mL 2.0106 mL
25 mM 0.0643 mL 0.3217 mL 0.6434 mL 1.6085 mL
30 mM 0.0536 mL 0.2681 mL 0.5362 mL 1.3404 mL
40 mM 0.0402 mL 0.2011 mL 0.4021 mL 1.0053 mL
50 mM 0.0322 mL 0.1609 mL 0.3217 mL 0.8043 mL
60 mM 0.0268 mL 0.1340 mL 0.2681 mL 0.6702 mL
80 mM 0.0201 mL 0.1005 mL 0.2011 mL 0.5027 mL
100 mM 0.0161 mL 0.0804 mL 0.1609 mL 0.4021 mL
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PD-M6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PD-M63058330-82-5mTORPROTACsAutophagyMammalian target of RapamycinHeLa cellsHepG2 liver cancer cellsliver cancerLAMTOR1MCF-7 breast cancer cellsCRBNbreast cancercervical cancerautophagyInhibitorinhibitorinhibit

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