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Cat. No.: HY-122857

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PKCiota-IN-1

PKCiota-IN-1 (compound 51) is a potent PKCiota (PKC-ι) inhibitor with an IC₅₀ of 2.7 nM. PKCiota-IN-1 also inhibits PKC-α and PKC-ε with IC₅₀s of 45 nM and 450 nM, respectively.

For research use only. We do not sell to patients.

CAS No.: 2230052-97-6
Formula: C₂₅H₂₂FN₅O
Molecular Weight:427.47

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

PKCiota-IN-1 Chemical Structure

CAS No.: 2230052-97-6

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Protocols (1)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

PKCι

2.7 nM (IC₅₀)

PKCα

45 nM (IC₅₀)

PKCε

450 nM (IC₅₀)

All PKC Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HCCLM3	GI₅₀	6.6 μM Compound: 51	Antiproliferative activity against human HCCLM3 cells after 72 hrs by CellTiter-Glo assay Antiproliferative activity against human HCCLM3 cells after 72 hrs by CellTiter-Glo assay	[PMID: 29688013]
Huh-7	GI₅₀	3.3 μM Compound: 51	Antiproliferative activity against human HuH7 cells after 72 hrs by CellTiter-Glo assay Antiproliferative activity against human HuH7 cells after 72 hrs by CellTiter-Glo assay	[PMID: 29688013]

Chemical Information

CAS No. 2230052-97-6
Molecular Weight 427.47
Formula C₂₅H₂₂FN₅O
SMILES

COC1=CC2=C(CNC(CF)C2)C=C1C3=C4C=C(C5=C6C(NC=C6)=NC=C5)C=CN4N=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jacek Kwiatkowski, et al. Fragment-Based Drug Discovery of Potent Protein Kinase C Iota Inhibitors. J Med Chem. 2018 May 24;61(10):4386-4396. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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