10047 Results for "

fluorescence quenching-based kinetic assay

" in MedChemExpress (MCE) Product Catalog:
Products (10047)

10047 Results for "fluorescence quenching-based kinetic assay" in MCE Product Catalog:

4206
Cell Counting Kit-8
Cell Counting Kit-8
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4206 Publications Verification
Cat. No.: HY-K0301

Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.The 5 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

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621
Lipopolysaccharides, from E. coli O55:B5
Lipopolysaccharides, from E. coli O55:B5
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621 Cited Publications
Cat. No.: HY-D1056
Synonyms: LPS, from Escherichia coli (O55:B5)
Category:  

Natural Products Microorganisms Disease Research Fields Inflammation/Immunology

Target:  

Toll-like Receptor (TLR)

Lipopolysaccharides, from E. coli O55:B5 (LPS, from Escherichia coli (O55:B5)) are endotoxins and TLR4 activators extracted from Escherichia coli (E. coli O55:B5) and are classified as S (smooth) type LPS. Lipopolysaccharides, from E. coli O55:B5 possess the typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activate TLR-4 in immune cells, exhibit high pyrogenicity, and demonstrate dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 can be widely used to induce cellular inflammation and establish animal models related to inflammation .
It is recommended to prepare a solution with concentration ≥2 mg/mL. Vortex thoroughly for more than 10 minutes. Due to the adsorption characteristics of LPS, silanized container or low adsorption centrifuge tubes should be used for aliquoting and storage, and mix thoroughly before use.
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387
Dimethyl sulfoxide, meets analytical specification of Ch.P.
Dimethyl sulfoxide, meets analytical specification of Ch.P.
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387 Cited Publications
Cat. No.: HY-Y0320
CAS No.: 67-68-5
Synonyms: DMSO, meets analytical specification of Ch.P.
Dimethyl sulfoxide (DMSO), meets analytical specification of Ch.P. is an aprotic solvent that dissolves polar and non-polar compounds, including water-insoluble therapeutic and toxic agents. Dimethyl sulfoxide (DMSO) has a strong affinity for water and can rapidly penetrate or enhance the penetration of other substances into biological membranes. Dimethyl sulfoxide also has potential free radical scavenging and anticholinesterase effects and may affect coagulation activity. Dimethyl sulfoxide also induces histamine release from mast cells but is thought to have low systemic toxicity. Dimethyl sulfoxide also exhibits antifreeze and antibacterial properties .
MCE provides Dimethyl sulfoxide that complies with the inspection standards (Ch.P) of Part 4 of the Chinese Pharmacopoeia (2020 Edition). Amicrobic, low endotoxin, can be used in various biochemical experiments such as drug dissolution.
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331
MitoSOX Red
MitoSOX Red
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331 Cited Publications
Cat. No.: HY-D1055
CAS No.: 1003197-00-9
MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems. The oxidized MitoSOX Red then binds to nucleic acids in mitochondria/nucleus, producing strong red fluorescence. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. In addition, superoxide dismutase (SOD) can prevent the oxidation of MitoSOX Red.
Excitation/emission wavelength: 510/580 nm.
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281
Ruxolitinib
Ruxolitinib
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281 Cited Publications
Cat. No.: HY-50856
CAS No.: 941678-49-5
Synonyms: INCB18424
Target:  

JAK Autophagy Mitophagy Apoptosis

Research Areas:  

Cancer

Ruxolitinib (INCB18424) is an orally active and selective JAK1/2 inhibitor with IC50s of 3.3 nM and 2.8 nM in cell-free assays, and has 130-fold selectivity for JAK1/2 over JAK3 . Ruxolitinib induces autophagy and kills tumor cells through toxic mitophagy .
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247
JC-1
JC-1
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247 Cited Publications
Cat. No.: HY-15534
CAS No.: 3520-43-2
Purity:  98.71%
Synonyms: CBIC2
JC-1 (CBIC2) is an ideal fluorescent probe widely used to detect mitochondrial membrane potential. JC-1 accumulates in mitochondria in a potential dependent manner and can be used to detect the membrane potential of cells or purified mitochondria. In normal mitochondria, JC-1 aggregates in the mitochondrial matrix to form a polymer, which emits strong red fluorescence (Ex=585 nm, Em=590 nm); When the mitochondrial membrane potential is low, JC-1 cannot aggregate in the matrix of mitochondria and produce green fluorescence (ex=510 nm, em= 527 nm) .
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224
PEG300
PEG300
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224 Cited Publications
Cat. No.: HY-Y0873
CAS No.: 25322-68-3
Synonyms: Polyethylene glycol 300
PEG300 (Polyethylene glycol 300), a neutral polymer of molecular weight 300, is a water-soluble, low immunogenic and biocompatible polymer formed by repeating units of ethylene glycol .
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196
Dihydroethidium
Dihydroethidium
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196 Cited Publications
Cat. No.: HY-D0079
CAS No.: 104821-25-2
Synonyms: Hydroethidine; PD-MY 003
Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation .
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194
Thiazolyl Blue
Thiazolyl Blue
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194 Cited Publications
Cat. No.: HY-15924
CAS No.: 298-93-1
Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .
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186
DAPI dihydrochloride
DAPI dihydrochloride
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186 Cited Publications
Cat. No.: HY-D0814
CAS No.: 28718-90-3
Synonyms: 4',6-Diamidino-2-phenylindole dihydrochloride
DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
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186
DAPI
DAPI
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186 Cited Publications
Cat. No.: HY-D2868
CAS No.: 47165-04-8
Synonyms: 4',6-Diamidino-2-phenylindole
DAPI (4',6-Diamidino-2-phenylindole) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
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180
DAPI dilactate
DAPI dilactate
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180 Cited Publications
Cat. No.: HY-D1738
CAS No.: 28718-91-4
Synonyms: 4',6-Diamidino-2-phenylindole dilactate
DAPI (4',6-Diamidino-2-phenylindole) dilactate is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining damaged and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm) .
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178
ATP dimagnesium
ATP dimagnesium
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178 Cited Publications
Cat. No.: HY-B2176B
CAS No.: 74804-12-9
Synonyms: Adenosine 5'-triphosphate dimagnesium
Category:  

Natural Products Endogenous metabolite Disease Research Fields Inflammation/Immunology

Target:  

Endogenous Metabolite NOD-like Receptor (NLR) Interleukin Related Bacterial

ATP (Adenosine 5'-triphosphate) dimagnesium is a central component of energy storage and metabolism in vivo. ATP dimagnesium provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP dimagnesium is an important endogenous signaling molecule in immunity and inflammation. ATP dimagnesium can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP dimagnesium has anti-bacterial infection effects and can protect mice against bacterial infection in mice .
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171
JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 Mitochondrial Membrane Potential Assay Kit
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171 Cited Publications
Cat. No.: HY-K0601

MCE JC-1 Mitochondrial Membrane Potential Assay Kit uses JC-1 to detect the mitochondrial membrane potential in variety of cell types, as well as intact tissues and isolated mitochondria.

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168
Propidium Iodide
Propidium Iodide
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168 Cited Publications
Cat. No.: HY-D0815
CAS No.: 25535-16-4
Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.
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168
SBE-β-CD
SBE-β-CD
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168 Cited Publications
Cat. No.: HY-17031
CAS No.: 182410-00-0
Synonyms: Sulfobutylether-β-Cyclodextrin
SBE-β-CD is a sulfobutylether β-cyclodextrin derivative used as an excipient or a formulating agent to increase the solubility of poorly soluble agents .
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168
ATP disodium salt, 100 mM Solution, PCR Grade
ATP disodium salt, 100 mM Solution, PCR Grade
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168 Cited Publications
Cat. No.: HY-B2176I
CAS No.: 987-65-5
Synonyms: Adenosine 5'-triphosphate disodium salt, 100 mM Solution, PCR Grade
ATP (Adenosine 5'-triphosphate) disodium salt, 100 mM Solution, PCR Grade is a central component of energy storage and metabolism in vivo. ATP disodium salt, 100 mM Solution, PCR Grade provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt, 100 mM Solution, PCR Grade is an important endogenous signaling molecule in immunity and inflammation. ATP disodium salt, 100 mM Solution, PCR Grade can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP disodium salt, 100 mM Solution, PCR Grade has anti-bacterial infection effects and can protect mice against bacterial infection in mice .
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167
BODIPY 581/591 C11
BODIPY 581/591 C11
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167 Cited Publications
Cat. No.: HY-D1301
CAS No.: 217075-36-0
BODIPY 581/591 C11 is a BODIPY borofluoroprene derivative with good light stability and low fluorescence artifacts. BODIPY 581/591 C11 can be used for study lipid peroxidation and antioxidant properties in living cells, or detect ferroptosis by reaction with hydroxyl radicals. BODIPY 581/591 C11 is emitted at 591 nm (reduced prototype), or redshifted to 510 nm (oxidized type). The excitation wavelengths were 581 nm (reduced prototype) and 500 nm (oxidized type) .
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165
Hoechst 33342
Hoechst 33342
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165 Cited Publications
Cat. No.: HY-15559
CAS No.: 23491-52-3
Purity:  99.94%
Synonyms: bisBenzimide H 33342; HOE 33342
Hoechst 33342 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution .
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165
Hoechst 33342 trihydrochloride
Hoechst 33342 trihydrochloride
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165 Cited Publications
Cat. No.: HY-15559A
CAS No.: 875756-97-1
Purity:  99.81%
Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride
Hoechst 33342 trihydrochloride is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution .
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