SOS1-IN-12
SOS1-IN-12 is a potent son of sevenless homolog 1 (SOS1) inhibitor with a Ki of 0.11 nM for SOS1 and an IC50 of 47 nM for pERK. SOS1-IN-13 can be used for researching anticancer.
For research use only. We do not sell to patients.
- CAS No.: 2654741-56-5
- Formula: C23H26F3N5
- Molecular Weight:429.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MKN-1 | IC50 |
46 nM
Compound: 18
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Antiproliferative activity against human MKN-1 cells assessed as reduction in cell viability measured after 48 hrs by 48 hrs by CCK-8 assay
Antiproliferative activity against human MKN-1 cells assessed as reduction in cell viability measured after 48 hrs by 48 hrs by CCK-8 assay
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[PMID: 37542991] |
| MKN-1 | IC50 |
46 nM
Compound: 18
|
Antiproliferative activity against human MKN-1 cells assessed as reduction in cell viability measured for 48 hrs by CCK-8 assay
Antiproliferative activity against human MKN-1 cells assessed as reduction in cell viability measured for 48 hrs by CCK-8 assay
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[PMID: 38430853] |
Chemical Information
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CAS No. 2654741-56-5
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Molecular Weight 429.48
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Formula C23H26F3N5
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SMILES
CC1=NN=C(C2=CC(N3CCNCC3)=CC=C21)N[C@@H](C4=CC=CC(C(F)(F)F)=C4C)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 100 mg/mL (232.84 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.82 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3284 mL | 11.6420 mL | 23.2840 mL | 58.2099 mL |
| 5 mM | 0.4657 mL | 2.3284 mL | 4.6568 mL | 11.6420 mL | |
| 10 mM | 0.2328 mL | 1.1642 mL | 2.3284 mL | 5.8210 mL | |
| 15 mM | 0.1552 mL | 0.7761 mL | 1.5523 mL | 3.8807 mL | |
| 20 mM | 0.1164 mL | 0.5821 mL | 1.1642 mL | 2.9105 mL | |
| 25 mM | 0.0931 mL | 0.4657 mL | 0.9314 mL | 2.3284 mL | |
| 30 mM | 0.0776 mL | 0.3881 mL | 0.7761 mL | 1.9403 mL | |
| 40 mM | 0.0582 mL | 0.2910 mL | 0.5821 mL | 1.4552 mL | |
| 50 mM | 0.0466 mL | 0.2328 mL | 0.4657 mL | 1.1642 mL | |
| 60 mM | 0.0388 mL | 0.1940 mL | 0.3881 mL | 0.9702 mL | |
| 80 mM | 0.0291 mL | 0.1455 mL | 0.2910 mL | 0.7276 mL | |
| 100 mM | 0.0233 mL | 0.1164 mL | 0.2328 mL | 0.5821 mL |
SOS1-IN-12 Related Classifications
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