2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. PPAR Reactive Oxygen Species (ROS)
  4. Soyasaponin Aa

Soyasaponin Aa is a soyasaponin that can be found in soy. Soyasaponin Aa suppresses PPARγ transcriptional activity, reduces PPARγ and C/EBPα expression, inhibits adipocyte differentiation and lipid accumulation, and reduces adipogenic marker gene expression. Soyasaponin Aa reduces hydrogen peroxide-induced reactive oxygen species (ROS) production and malondialdehyde levels. Soyasaponin Aa exhibits cellular antioxidant activity. Soyasaponin Aa can be used for the research of obesity.

For research use only. We do not sell to patients.

Soyasaponin Aa

Soyasaponin Aa Chemical Structure

CAS No. : 117230-33-8

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Based on 1 publication(s) in Google Scholar

Other Forms of Soyasaponin Aa:

Soyasaponin Aa Related Antibodies

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1 Publications Citing Use of MCE Soyasaponin Aa

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Description

Soyasaponin Aa is a soyasaponin that can be found in soy. Soyasaponin Aa suppresses PPARγ transcriptional activity, reduces PPARγ and C/EBPα expression, inhibits adipocyte differentiation and lipid accumulation, and reduces adipogenic marker gene expression. Soyasaponin Aa reduces hydrogen peroxide-induced reactive oxygen species (ROS) production and malondialdehyde levels. Soyasaponin Aa exhibits cellular antioxidant activity. Soyasaponin Aa can be used for the research of obesity[1].

In Vitro

Soyasaponin Aa (12.5-200 μM; 24 h) shows no significant cytotoxicity toward 3T3-L1 preadipocytes[1].
Soyasaponin Aa (25-100 μM; 8 days) dose-dependently inhibits lipid accumulation during 3T3-L1 adipocyte differentiation, leaving 75% of control lipid content remaining at 100 μM[1].
Soyasaponin Aa (50-100 μM; 8 days) downregulates PPARγ and C/EBPα mRNA expression in fully differentiated 3T3-L1 adipocytes, with statistically significant suppression observed at 100 μM[1].
Soyasaponin Aa (50-100 μM; 8 days) downregulates the mRNA expression of adipogenic marker genes (adiponectin, ADD1/SREBP1c, aP2, Fas, resistin) in fully differentiated 3T3-L1 adipocytes[1].
Soyasaponin Aa (50-100 μM; 8 days) significantly suppresses PPARγ and C/EBPα protein expression in fully differentiated 3T3-L1 adipocytes[1].
Soyasaponin Aa (25-100 μM; 24 h) significantly inhibits PPARγ transcriptional activity in HEK 293T cells[1].
Soyasaponin Aa (25-400 μg/mL; 24 h) exhibits no cytotoxicity to human HepG2 cells at concentrations up to 100 μg/mL[2].
Soyasaponin Aa (100 μg/mL; 24 h) protects human HepG2 cells from hydrogen peroxide-induced apoptotic morphological changes[2].
Soyasaponin Aa (100 μg/mL; 24 h) reduces hydrogen peroxide-induced ROS generation in human HepG2 cells, lowering ROS content to 58.7%[2].
Soyasaponin Aa (100 μg/mL; 24 h) reduces hydrogen peroxide-induced MDA levels and increases SOD, GSH-Px, and CAT antioxidant enzyme activities in human HepG2 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Soyasaponin Aa Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: 3T3-L1 preadipocytes
Concentration: 12.5; 25; 50; 100; 200 μM
Incubation Time: 24 h
Result: Showed no significant cytotoxic effects on 3T3-L1 cells, with relative cell survival remaining near control levels.

Real Time qPCR[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 50; 100 μM
Incubation Time: 8 days
Result: Did not significantly suppress PPARγ mRNA expression at 50 μM.
Caused a statistically significant suppression of both PPARγ and C/EBPα mRNA expression relative to differentiated controls at 100 μM.\n
Caused a marked, statistically significant reduction in the mRNA expression of adiponectin, ADD1/SREBP1c, aP2, Fas, and resistin relative to differentiated controls at 50 μM and 100 μM, with the effect being dose-dependent for most genes.

Western Blot Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 50; 100 μM
Incubation Time: 8 days
Result: Did not significantly suppress PPARγ or C/EBPα protein expression at 50 μM.
Caused a statistically significant suppression of both PPARγ and C/EBPα protein expression relative to differentiated controls, with densitometric analysis confirming reduced protein levels.

Cell Viability Assay[2]

Cell Line: human HepG2 cells
Concentration: 25; 50; 100; 200; 400 μg/mL
Incubation Time: 24 h
Result: Showed a concentration-dependent effect on HepG2 cell viability.
Maintained cell survival rate at 92.56% at 100 μg/mL, with no cytotoxicity observed.
Increased cytotoxicity at concentrations over 200 μg/mL.
Molecular Weight

1365.46

Formula

C64H100O31
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@](CC=C3[C@]2(CC[C@]4(C)[C@@]3([H])CC(C)(C)[C@@H](O)[C@H]4O[C@@]5([H])[C@@H]([C@H]([C@@H](O)CO5)O[C@@]6([H])[C@@H]([C@H]([C@H](OC(C)=O)CO6)OC(C)=O)OC(C)=O)O)C)([H])[C@@]7([C@@]([C@](C)([C@@H](O[C@@]8([H])[C@@H]([C@H]([C@H](O)[C@@H](C(O)=O)O8)O)O[C@@]9([H])[C@@H]([C@H]([C@@H](O)[C@@H](CO)O9)O)O[C@]%10([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]%10O)CO)CC7)CO)([H])CC1)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (73.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7324 mL 3.6618 mL 7.3235 mL
5 mM 0.1465 mL 0.7324 mL 1.4647 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7324 mL 3.6618 mL 7.3235 mL 18.3088 mL
5 mM 0.1465 mL 0.7324 mL 1.4647 mL 3.6618 mL
10 mM 0.0732 mL 0.3662 mL 0.7324 mL 1.8309 mL
15 mM 0.0488 mL 0.2441 mL 0.4882 mL 1.2206 mL
20 mM 0.0366 mL 0.1831 mL 0.3662 mL 0.9154 mL
25 mM 0.0293 mL 0.1465 mL 0.2929 mL 0.7324 mL
30 mM 0.0244 mL 0.1221 mL 0.2441 mL 0.6103 mL
40 mM 0.0183 mL 0.0915 mL 0.1831 mL 0.4577 mL
50 mM 0.0146 mL 0.0732 mL 0.1465 mL 0.3662 mL
60 mM 0.0122 mL 0.0610 mL 0.1221 mL 0.3051 mL
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Soyasaponin Aa Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soyasaponin Aa117230-33-8PPARReactive Oxygen Species (ROS)Peroxisome proliferator-activated receptorsHEK 293T cellscatalaseHepG2 cellssuperoxide dismutaseC/EBPαPPARγadipocytesglutathione peroxidasereactive oxygen species3T3-L1 preadipocytesInhibitorinhibitorinhibit

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