2. Metabolic Enzyme/Protease PI3K/Akt/mTOR Stem Cell/Wnt Immunology/Inflammation NF-κB Apoptosis
  3. Aldose Reductase Akt GSK-3 β-catenin Reactive Oxygen Species (ROS) Mitochondrial Metabolism Apoptosis c-Myc
  4. Soyasaponin Ba

Soyasaponin Ba is a soyasaponin that can be isolated from Phaseolus vulgaris, acts as an aldose reductase inhibitor (ARI). Soyasaponin Ba activates Akt/GSK3β/β-catenin signaling pathway, reduces lipid accumulation, lowers ROS generation, improves mitochondrial membrane potential, ATP levels, and morphology, and inhibits apoptosis. Soyasaponin Ba can be used for the research of lipid accumulation and secondary diabetic complications.

For research use only. We do not sell to patients.

Soyasaponin Ba

Soyasaponin Ba Chemical Structure

CAS No. : 114590-20-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Soyasaponin Ba:

Soyasaponin Ba Related Antibodies

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1 Publications Citing Use of MCE Soyasaponin Ba

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GSK-3 GSK-3α GSK-3β

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Description

Soyasaponin Ba is a soyasaponin that can be isolated from Phaseolus vulgaris, acts as an aldose reductase inhibitor (ARI). Soyasaponin Ba activates Akt/GSK3β/β-catenin signaling pathway, reduces lipid accumulation, lowers ROS generation, improves mitochondrial membrane potential, ATP levels, and morphology, and inhibits apoptosis. Soyasaponin Ba can be used for the research of lipid accumulation and secondary diabetic complications[1][2].

In Vitro

Soyasaponin Ba (0.78-25 μM; 0-600 s) directly binds to purified AKT1 protein with a Kd of 4.19 μM[1].
Soyasaponin Ba (4-32 μM; 24 h) dose-dependently alleviates FFA-induced intracellular ROS accumulation, and improves FFA-induced mitochondrial dysfunction in HepG2 cells[1].
Soyasaponin Ba (4-32 μM; 24 h) dose-dependently alleviates FFA-induced apoptosis in HepG2 cells[1].
Soyasaponin Ba (16-32 μM; 24 h) activates the Akt/GSK3β/β-catenin signaling pathway in FFA-induced HepG2 cells[1].
Soyasaponin Ba (8-32 μM; 24 h) dose-dependently alleviates FFA-induced lipid accumulation in THLE-2 cells[1].
Soyasaponin Ba (16-32 μM; 24 h) dose-dependently alleviates FFA-induced ROS accumulation, mitochondrial dysfunction, and apoptosis in THLE-2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Soyasaponin Ba Related Antibodies

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 4; 8; 16; 32 μM
Incubation Time: 24 h
Result: Reduced FFA-induced apoptosis in a concentration-dependent manner, as shown by decreased green fluorescence intensity (cleaved caspase-3 activity) in single-cell imaging, normalized relative fluorescent units (RFU) in bulk culture, and reduced Annexin V-mCherry staining.
Significantly decreased caspase-3 activity at 16 and 32 μM, with effects comparable to 20 μM atorvastatin.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 16; 32 μM
Incubation Time: 24 h
Result: Restored phosphorylated Akt and phosphorylated GSK3β levels reduced by FFA treatment at 32 μM.
Increased intracellular β-catenin and c-Myc levels reduced by FFA treatment in a concentration-dependent manner at 16 and 32 μM.

Immunofluorescence[1]

Cell Line: HepG2 cells
Concentration: 4; 8; 16; 32 μM
Incubation Time: 24 h
Result: Dose-dependently alleviates FFA-induced lipid accumulation in HepG2 cells.
Showed significant effects at all tested concentrations.
In Vivo

Soyasaponin Ba (16-128 μM; incorporated into nematode growth medium; every 48 hours; 3-4 total doses; from L4 stage) dose-dependently alleviates high-glucose induced lipid accumulation and oxidative stress in wild-type N2 C. elegans[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: wild-type N2 elegans[1]
Dosage: 16; 32; 64; 128 μM
Administration: incorporated into nematode growth medium; every 48 hours; 3-4 total doses; from L4 stage
Result: Dose-dependently reduced high-glucose induced lipid accumulation.
At 128 μM, reduced Nile Red fluorescence intensity significantly compared to the high-glucose untreated group.
Dose-dependently reduced high-glucose induced ROS accumulation.
Molecular Weight

959.12

Formula

C48H78O19
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@H]1O[C@@]2([H])[C@H](O[C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)[C@H](O)[C@@H](C(O)=O)O[C@@]1([H])O[C@@H]4[C@@](CO)(C)[C@]5([H])CC[C@@]6(C)[C@]7(C)CC[C@@]8(C)[C@H](O)CC(C)(C)C[C@@]8([H])C7=CC[C@]6([H])[C@@]5(C)CC4
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (260.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0426 mL 5.2131 mL 10.4262 mL
5 mM 0.2085 mL 1.0426 mL 2.0852 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Purity & Documentation

Purity: 99.47%

SDS (252 KB)

COA (258 KB) HNMR (130 KB) RP-HPLC (182 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0426 mL 5.2131 mL 10.4262 mL 26.0656 mL
5 mM 0.2085 mL 1.0426 mL 2.0852 mL 5.2131 mL
10 mM 0.1043 mL 0.5213 mL 1.0426 mL 2.6066 mL
15 mM 0.0695 mL 0.3475 mL 0.6951 mL 1.7377 mL
20 mM 0.0521 mL 0.2607 mL 0.5213 mL 1.3033 mL
25 mM 0.0417 mL 0.2085 mL 0.4170 mL 1.0426 mL
30 mM 0.0348 mL 0.1738 mL 0.3475 mL 0.8689 mL
40 mM 0.0261 mL 0.1303 mL 0.2607 mL 0.6516 mL
50 mM 0.0209 mL 0.1043 mL 0.2085 mL 0.5213 mL
60 mM 0.0174 mL 0.0869 mL 0.1738 mL 0.4344 mL
80 mM 0.0130 mL 0.0652 mL 0.1303 mL 0.3258 mL
100 mM 0.0104 mL 0.0521 mL 0.1043 mL 0.2607 mL
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Soyasaponin Ba Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soyasaponin Ba114590-20-4Aldose ReductaseAktGSK-3β-cateninReactive Oxygen Species (ROS)Mitochondrial MetabolismApoptosisc-MycPKBProtein kinase BGlycogen synthase kinase-3Glycogen synthase kinase 3Beta cateninMycAkt/GSK3β/β-catenin signaling pathwaylipid accumulationwild-type N2 C. elegansHepG2 cellsAKT1hAKR1B1apoptosisTHLE-2 cellsmitochondrial functionreactive oxygen speciesInhibitorinhibitorinhibit

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