Sp-cAMPS sodium salt 

Sp-cAMPS sodium salt, a cAMP analog, is potent activator of cAMP-dependent PKA I and PKA II. Sp-cAMPS sodium salt is also a potent, competitive phosphodiesterase (PDE3A) inhibitor with a K_i of 47.6 µM. Sp-cAMPS sodium salt binds the PDE10 GAF domain with an EC₅₀ of 40 μM^[1][2][3].

Treatment of hepatocytes with Sp-cAMPS sodium salt, the stimulatory diastereomer of adenosine cyclic 3',5'-phosphorothioate, mimics the response seen with glucagon. The glucagon-stimulated increases in the level of Ca²⁺ can be mimicked by Sp-cAMPS sodium salt^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In chronic alcohol consumption (CAC) mice, direct infusion of the Sp-cAMPS (1 µg/µL) sodium salt into the prefrontal cortex significantly improves or impairs, respectively, working memory performance in withdrawn and water animals^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

367.25

Solid

C₁₀H₁₁N₅NaO₅PS

142439-95-0

O[[email protected]]1[[email protected]@H](O[[email protected]@]2([H])[[email protected]@]1([H])O[[email protected]](OC2)([S])=O)N3C4=C(C(N)=NC=N4)N=C3.[Na+].[-]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Su H Hung, et al. A new nonhydrolyzable reactive cAMP analog, (Sp)-adenosine-3',5'-cyclic-S-(4-bromo-2,3-dioxobutyl)monophosphorothioate irreversibly inactivates human platelet cGMP-inhibited cAMP phosphodiesterase. Bioorg Chem. 2002 Feb;30(1):16-31.  [Content Brief]

  [2]. L Y Wang, et al. Regulation of kainate receptors by cAMP-dependent protein kinase and phosphatases. Science. 1991 Sep 6;253(5024):1132-5.  [Content Brief]

  [3]. Ronald Jäger, et al. Activation of PDE10 and PDE11 phosphodiesterases. J Biol Chem. 2012 Jan 6;287(2):1210-9.  [Content Brief]

  [4]. P A Connelly,et al. A study of the mechanism of glucagon-induced protein phosphorylation in isolated rat hepatocytes using (Sp)-cAMPS and (Rp)-cAMPS, the stimulatory and inhibitory diastereomers of adenosine cyclic 3',5'-phosphorothioate. J Biol Chem. 1987 Mar 25;262(9):4324-32.  [Content Brief]

  [5]. G Dominguez, et al. Rescuing prefrontal cAMP-CREB pathway reverses working memory deficits during withdrawal from prolonged alcohol exposure. Brain Struct Funct. 2016 Mar;221(2):865-77.  [Content Brief]