Sulfamethoxazole-NO
Based on 1 Customer Validation
Sulfamethoxazole-NO (SMX-NO) is a haptenizing immunogen. Sulfamethoxazole-NO reduces NF-kB signaling, induces AP-1 signaling, and depletes cysteine, glutathione, and ascorbic acid. Sulfamethoxazole-NO forms covalently haptenized proteins and antigens, activates MHC-restricted T cells and CD4+ T cells, and upregulates dendritic cell CD40. Sulfamethoxazole-NO induces apoptosis, necrosis, methemoglobin formation, and cytotoxicity accompanied by loss of membrane integrity. Sulfamethoxazole-NO inhibits neutrophil function and oxidative metabolism. Sulfamethoxazole-NO can be used for research on hypersensitivity reactions.
For research use only. We do not sell to patients.
- CAS No.: 29699-89-6
- Formula: C10H9N3O5S
- Molecular Weight:283.26
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
All AP-1 Isoforms
More
Biological Activity
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| J774 | IC50 |
>256 μM
Compound: 4Da
|
Cytotoxicity against mouse J774 cells after 24 hrs by by resazurin reduction test
Cytotoxicity against mouse J774 cells after 24 hrs by by resazurin reduction test
|
[PMID: 19811921] |
In Vitro
Sulfamethoxazole-NO (1-1000 μM; 1 h) decreases NF-kB and induces AP-1 DNA binding at 1000 μM in human lymphocytes[1].
Sulfamethoxazole-NO (0.5-4000 µM; 24 h) selectively upregulates CD40 on human monocyte-derived dendritic cells without affecting CD80, CD83, or CD86, with the maximal effect at 1 µM[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human neutrophils
-
Concentration:1-1000 μM
-
Incubation Time:1-8 h
-
Result:Induced apoptosis in human neutrophils at 100-300 μM.
In Vivo
Sulfamethoxazole-NO (100 mg/kg; intraperitoneal injection; single injection) causes haptenation of antigen-presenting cells in preference to T cells in healthy male Lewis rats[4].
Sulfamethoxazole-NO (1 mg/kg; intraperitoneal injection; 4 times per week; for 2 weeks) immunologically induces splenocyte proliferative responses to ex vivo Sulfamethoxazole-NO in CD1 mice[5].
Sulfamethoxazole-NO (1 mg/kg; intraperitoneal injection; 4 times per week; for 2 weeks) immunologically induces a proliferative response of splenocytes to ex vivo Sulfamethoxazole-NO in Wistar rats[5].
Sulfamethoxazole-NO (1 mg/kg; intraperitoneal injection; 4 times per week; for 2 weeks) immunizes New Zealand white rabbits to induce proliferative responses of splenocytes and lymphocytes to isolated Sulfamethoxazole-NO, and the lymphocyte response persists for at least 4 months, with no cross-reactivity with SMX[5].
Sulfamethoxazole-NO (1 mg/kg; subcutaneous injection; days 0, 1, and 2) administration induces lymph node cell proliferation in BALB/C mice, and this proliferation is eliminated by CD40L blockade[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Beagle (purpose-bred female, 7-10 months old, 7.0-9.4 kg)[3]
-
Dosage:1 mg/kg; 3 mg/kg; 10 mg/kg; vehicle control
-
Administration:i.p.; once daily for four consecutive days per week for 2 weeks; 1 additional week observation
-
Result:Lowered the baseline levels of reduced glutathione in white blood cells.
Increased the baseline levels of plasma cysteine.
Decreased plasma ascorbic acid levels in the 10 mg/kg dosage group during the second week of administration.
-
Animal Model:Lewis (male)[4]
-
Dosage:100 mg/kg
-
Administration:i.p.; single injection
-
Result:Led to preferential haptenation of antigen-presenting cells over T cells.
- Phagocytosis Functional Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- Apoptosis Solutions
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
-
CAS No. 29699-89-6
-
Appearance Solid
-
Molecular Weight 283.26
-
Formula C10H9N3O5S
-
Color White to off-white
-
SMILES
O=S(C1=CC=C([N+]([O-])=O)C=C1)(NC2=NOC(C)=C2)=O
-
Synonyms
SMX-NO
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (353.03 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
-
Data Sheet (291 KB)
-
SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[3]. Lavergne SN, et al. Evaluation of the clinical, immunologic, and biochemical effects of nitroso sulfamethoxazole administration to dogs: a pilot study. Toxicology. 2005 Mar 01;208(1):63-72. [Content Brief]
[7]. Roujeau JC. Immune mechanisms in drug allergy. Allergology International. 2006 Jan 1;55(1):27-33.
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5303 mL | 17.6516 mL | 35.3033 mL | 88.2581 mL |
| 5 mM | 0.7061 mL | 3.5303 mL | 7.0607 mL | 17.6516 mL | |
| 10 mM | 0.3530 mL | 1.7652 mL | 3.5303 mL | 8.8258 mL | |
| 15 mM | 0.2354 mL | 1.1768 mL | 2.3536 mL | 5.8839 mL | |
| 20 mM | 0.1765 mL | 0.8826 mL | 1.7652 mL | 4.4129 mL | |
| 25 mM | 0.1412 mL | 0.7061 mL | 1.4121 mL | 3.5303 mL | |
| 30 mM | 0.1177 mL | 0.5884 mL | 1.1768 mL | 2.9419 mL | |
| 40 mM | 0.0883 mL | 0.4413 mL | 0.8826 mL | 2.2065 mL | |
| 50 mM | 0.0706 mL | 0.3530 mL | 0.7061 mL | 1.7652 mL | |
| 60 mM | 0.0588 mL | 0.2942 mL | 0.5884 mL | 1.4710 mL | |
| 80 mM | 0.0441 mL | 0.2206 mL | 0.4413 mL | 1.1032 mL | |
| 100 mM | 0.0353 mL | 0.1765 mL | 0.3530 mL | 0.8826 mL |
Sulfamethoxazole-NO Related Classifications
HY-W587780 Related Classifications
Powered by Bioz