TFAM activator 1
Based on 1 Customer Validation
TFAM activator 1 is a TFAM activator. TFAM activator 1 increases TFAM protein levels, promotes mitochondrial DNA stability, increases mitochondrial DNA copy number, and prevents mitochondrial DNA from escaping into the cytoplasm. TFAM activator 1 improves cellular energy metabolism in cybrid cells. TFAM activator 1 reduces fibrosis markers in fibroblasts. TFAM activator 1 can be used in the research of mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes syndrome, systemic sclerosis, and autoimmune diseases.
For research use only. We do not sell to patients.
- Purity: 99.25%
- CAS No.: 932992-44-4
- Formula: C18H19N3O4S
- Molecular Weight:373.43
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
TFAM activator 1 (compound 2) (0-25 μM; 5 days) increases mtDNA copy number in T47D, A549, 22RV1, A172 and HeLa cells[1].
TFAM activator 1 (3-10 μM; 48 h) inhibits CXCL10 protein secretion in a dose-dependent manner in TNF-α-stimulated THP-1 cells[1].
TFAM activator 1 (1-10 μM; 5 days) dose-dependently increases the TFAM protein level in T47D cells[1].
TFAM activator 1 (0.2-20 μM; 48 h) dose-dependently reduces the fibrosis markers fibronectin and α-smooth muscle actin in fibroblasts from patients with systemic sclerosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:T47D
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Concentration:1 μM, 3 μM, 10 μM
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Incubation Time:5 days
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Result:Increased TFAM protein levels in a dose-dependent manner.
Chemical Information
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CAS No. 932992-44-4
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Appearance Solid
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Molecular Weight 373.43
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Formula C18H19N3O4S
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Color White to off-white
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SMILES
CN1C(OC2=CC(S(=O)(N3CCC(CC3)C4=CC=NC=C4)=O)=CC=C21)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 25 mg/mL (66.95 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.69 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6779 mL | 13.3894 mL | 26.7788 mL | 66.9470 mL |
| 5 mM | 0.5356 mL | 2.6779 mL | 5.3558 mL | 13.3894 mL | |
| 10 mM | 0.2678 mL | 1.3389 mL | 2.6779 mL | 6.6947 mL | |
| 15 mM | 0.1785 mL | 0.8926 mL | 1.7853 mL | 4.4631 mL | |
| 20 mM | 0.1339 mL | 0.6695 mL | 1.3389 mL | 3.3473 mL | |
| 25 mM | 0.1071 mL | 0.5356 mL | 1.0712 mL | 2.6779 mL | |
| 30 mM | 0.0893 mL | 0.4463 mL | 0.8926 mL | 2.2316 mL | |
| 40 mM | 0.0669 mL | 0.3347 mL | 0.6695 mL | 1.6737 mL | |
| 50 mM | 0.0536 mL | 0.2678 mL | 0.5356 mL | 1.3389 mL | |
| 60 mM | 0.0446 mL | 0.2232 mL | 0.4463 mL | 1.1158 mL |
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