Thymohydroquinone
Based on 1 Customer Validation
Thymohydroquinone is a COX-2 inhibitor and anti-SARS-CoV-2 agent that induces cytotoxicity, antiproliferative effects and inhibits tumor growth. Thymohydroquinone cannot scavenge superoxide radicals via σ (hydrogen atom transfer) and π-π attacks with superoxide anions. Thymohydroquinone can be used in research related to squamous cell carcinoma, fibrosarcoma and coronavirus disease 2019 (SARS-CoV-2 infection).
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 2217-60-9
- Formula: C10H14O2
- Molecular Weight:166.22
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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COX-2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A2780 | IC50 |
3.1 μM
Compound: 18
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Growth inhibition of human A2780 cells after 72 hrs by SRB assay
Growth inhibition of human A2780 cells after 72 hrs by SRB assay
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[PMID: 29519737] |
| A2780cis | IC50 |
9.8 μM
Compound: 18
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Growth inhibition of human A2780cis cells after 72 hrs by SRB assay
Growth inhibition of human A2780cis cells after 72 hrs by SRB assay
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[PMID: 29519737] |
| OVCAR-8 | IC50 |
8.9 μM
Compound: 18
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Growth inhibition of human OVCAR8 cells after 72 hrs by SRB assay
Growth inhibition of human OVCAR8 cells after 72 hrs by SRB assay
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[PMID: 29519737] |
Thymohydroquinone (0.01-0.1 mg/mL; 24 h) exhibits dose-dependent, statistically significant cytotoxic activity against L929 mouse fibroblast cells, SCC VII mouse squamous cell carcinoma cells, and FsaR mouse fibrosarcoma cells[1].
Thymohydroquinone inhibits SARS-CoV-2 in VERO-E6 cells with an IC50 of 23.15 ng/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:L929 mouse fibroblasts, SCC VII mouse squamous cell carcinoma cells, FsaR mouse fibrosarcoma cells
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Concentration:0.01-0.1 mg/mL
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Incubation Time:24 h
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Result:Induced 21% cell growth inhibition in L929 cells, 17% cell growth inhibition in SCC VII cells, and 15% cell growth inhibition in FsaR cells at 0.01 mg/mL.
Induced 63% cell growth inhibition in L929 cells, 87% cell growth inhibition in SCC VII cells, and 92% cell growth inhibition in FsaR cells at 0.1 mg/mL.
Showed statistically significant (p < 0.01) dose-dependent cytotoxic activity, with greater inhibition against tumor cell lines (SCC VII, FsaR) than against normal L929 fibroblasts at the 0.1 mg/mL concentration.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C3HHf/BuZgr/Hr (male, 3 months old; fibrosarcoma or squamous cell carcinoma cell subcutaneous transplantation)[1]
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Dosage:5 mg/kg per injection; total cumulative dose of 20 mg/kg
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Administration:intratumoral; on days 3, 4, 5, 11 post-tumor cell inoculation
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Result:Achieved a tumor growth inhibition (TGI) of up to 49% against SCC VII tumors and up to 33% against FsaR tumors.
Showed statistically significant differences in tumor growth compared with control mice (p < 0.05) at multiple time points post-inoculation.
Exhibited no statistically significant difference in in vivo antitumor activity compared with thymoquinone.
Chemical Information
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CAS No. 2217-60-9
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Appearance Solid
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Molecular Weight 166.22
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Formula C10H14O2
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Color White to off-white
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SMILES
OC1=CC(C(C)C)=C(O)C=C1C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 1 mg/mL (6.02 mM; ultrasonic and warming and heat to 60°C)
DMSO : 1 mg/mL (6.02 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ivankovic S, et al. The antitumor activity of thymoquinone and thymohydroquinone in vitro and in vivo. Exp Oncol. 2006;28(3):220-224. [Content Brief]
[2]. Esharkawy ER, et al. In vitro potential antiviral SARS-CoV-19- activity of natural product thymohydroquinone and dithymoquinone from Nigella sativa. Bioorg Chem. 2022;120:105587. [Content Brief]
[3]. Sakib R, et al. Antioxidant Properties of Thymoquinone, Thymohydroquinone and Black Cumin (Nigella sativa L.) Seed Oil: Scavenging of Superoxide Radical Studied Using Cyclic Voltammetry, DFT and Single Crystal X-ray Diffraction. Antioxidants (Basel). 2023;12(3):607. Published 2023 Mar 1. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 6.0161 mL | 30.0806 mL | 60.1612 mL | 150.4031 mL |
| 5 mM | 1.2032 mL | 6.0161 mL | 12.0322 mL | 30.0806 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Thymohydroquinone Related Classifications
HY-W196368 Related Classifications
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