Cat. No.: HY-17494B

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Timolol hemimaleate

Name: Timolol hemimaleate
Brand: MedChemExpress
SKU: HY-17494B
Availability: PreOrder

(Synonyms: (S)-L-714,465 hemimaleate; MK 950 hemimaleate)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Timolol ((S)-L-714,465; MK 950) hemimaleate is a β-blocker available for both topical and systemic administration. Topical Timolol hemimaleate is primarily used to reduce intraocular pressure with open-angle glaucoma and ocular hypertension. Timolol hemimaleate can also be used for the research of infantile hemangiomas, hypertension, myocardial infarction, migraine prophylaxis, and atrial fibrillation.Timolol also has cardioprotective effect.

For research use only. We do not sell to patients.

CAS No.: 33305-95-2
Formula: C₁₃H₂₄N₄O₃S.1/2C₄H₄O₄
Molecular Weight:374.45

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (277 KB)

Timolol hemimaleate Chemical Structure

CAS No.: 33305-95-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References
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Biological Activity

Description

All Adrenergic Receptor Isoforms :

In Vitro

Timolol hemimaleate can significantly prevent the increased lipid peroxidation level of the heart from diabetic rats. Timolol hemimaleate can induce a well-balanced ratio between oxidative stress and antioxidant defense system in the diabetic animals, can have an important cardioprotection against diabetes-induced ERS and associated apoptotic effects^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Timolol hemimaleate has a cardioprotective effect via inhibition of ERS response in diabetic rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

3-month old male Wistar rats^[3].
Dosage:

5 mg/kg.
Administration:

Timolol (5 mg/kg daily for 12-week)
Result:

Showed cardioprotective effect.

Chemical Information

CAS No. 33305-95-2
Molecular Weight 374.45
Formula C₁₃H₂₄N₄O₃S.1/2C₄H₄O₄
SMILES

OC(/C=C\C(O)=O)=O.CC(C)(C)NC[C@H](O)COC1=NSN=C1N2CCOCC2.[1/2]
Synonyms

(S)-L-714,465 hemimaleate; MK 950 hemimaleate
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (6)

All Cell Proliferation/Viability AssayIn Vivo Efficacy StudyCell Imaging/StainingHistological Imaging/StainingIFWB

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Adv Mater

Colloid-Forming Prodrug-Hydrogel Composite Prolongs Lower Intraocular Pressure in Rodent Eyes after Subconjunctival Injection. [Abstract]

2025 Feb;37(8):e2419306. PMID: 39763100

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Feb;37(8):e2419306. [Abstract]

Live-cell percentages were assessed by calcein-AM and Hoechst staining in hTCEpi cells after treatment with Timolol maleate ((S)-Timolol Maleate; 0.137% w/v; 24 h). The Timolol maleate group showed a significantly lower percentage of live cells.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Feb;37(8):e2419306. [Abstract]

IOP was monitored over 24 h and 56 d in rats after a single subconjunctival injection of soluble Timolol maleate ((S)-Timolol Maleate; 0.137% w/v, 30 μL). Timolol maleate significantly reduced IOP at 6 h, but the effect was no longer significant at 24 h.
Adv Funct Mater

2026 Jun 11.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Jun 11.

IOP was monitored in New Zealand white rabbits after a single subconjunctival injection of (S)-timolol maleate solution or DSGS-T (DSGS loaded with (S)-timolol maleate) (Timolol maleate; both 1 mg/mL, 0.1 mL per eye; single dose). Timolol maleate and DSGS-T significantly decreased IOP compared with saline.
Protein Cell

Identification of serotonin 2A receptor as a novel HCV entry factor by a chemical biology strategy. [Abstract]

2019 Mar;10(3):178-195. PMID: 29542010

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Protein Cell. 2019 Mar;10(3):178-195. [Abstract]

Cell viability was assessed in Huh7.5.1 cells after treatment with (S)-timolol maleate ((S)-timolol; 10 μM; 48 h) and expressed relative to 0.5% DMSO-treated control cells.
Redox Biol

Oxidative stress promotes lipid-laden macrophage formation via CYP1B1. [Abstract]

2025 Feb:79:103481. PMID: 39721495
Mater Today Bio

Transdermal delivery of timolol maleate using hydrogel microneedles for the treatment of infantile haemangiomas. [Abstract]

2025 Apr 12:32:101752. PMID: 40290897

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Apr 12:32:101752. [Abstract]

Live-dead cell staining demonstrates the effects of different timolol maleate ((S)-Timolol maleate,TIM; 600-1500 μM; 24 h) concentrations on the cell morphology of 3D-cultured HemECs. Compared to control group, TIM-treated HemECs showed the disappearance of filopodia, exhibited a rounded shape, and had a significant reduction in cell count.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Apr 12:32:101752. [Abstract]

Cell viability was assessed by CCK-8 in 3D-cultured HemECs after treatment with Timolol maleate ((S)-Timolol maleate,TIM; 600-1500 μM; 24 h) and in 2D-cultured HemECs after treatment with TIM (10-10000 mM; 24 or 48 h). TIM inhibited HemEC growth in a concentration-dependent manner.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Apr 12:32:101752. [Abstract]

Hemangioma appearance and volume were evaluated in mouse with haemangiomas and tumour grafts after treatment for 2 weeks with Free TIM ((S)-Timolol maleate; 0.5%, 40 μL/cm²; topical administration; three times daily) or BSAMA-TIM-MNs (9 mg/mL; transdermal microneedles; replaced every 3 d). BSAMA-TIM-MNs controlled hemangioma volume more effectively than Free TIM or blank microneedles and produced a decreasing trend in hemangioma size.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Apr 12:32:101752. [Abstract]

H&E staining of hemangiomas from mouse with haemangiomas and tumour grafts after treatment for 2 weeks with Free TIM ((S)-Timolol maleate; 0.5%, 40 μL/cm²; topical administration; three times daily) or BSAMA-TIM-MNs (9 mg/mL; transdermal microneedles; replaced every 3 d). H & E staining of the haemangioma tissue revealed a reduced number of microvessels in the BSAMA-TIM-MNs group compared to those in the other three groups.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Apr 12:32:101752. [Abstract]

GLUT-1 immunofluorescence staining and fluorescence intensity in mouse with haemangiomas and tumour grafts after treatment for 2 weeks with Free TIM ((S)-Timolol maleate; 0.5%, 40 μL/cm²; topical administration; three times daily) or BSAMA-TIM-MNs (9 mg/mL; transdermal microneedles; replaced every 3 d). The fluorescence intensity of GLUT-1 was weaker after treatment with BSAMA-TIM-MNs than in the other three groups.

Timolol hemimaleate purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Apr 12:32:101752. [Abstract]

GLUT-1 expression was examined by western blotting in mouse with haemangiomas and tumour grafts after treatment for 2 weeks with Free TIM ((S)-Timolol maleate; 0.5%, 40 μL/cm²; topical administration; three times daily) or BSAMA-TIM-MNs (9 mg/mL; transdermal microneedles; replaced every 3 d). GLUT-1 expression was reduced by treatment with BSAMA-TIM-MNs and Free TIM.
Patent

US20230090708A1.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. James Barnes; Majid Moshirfar. Timolol.

[2]. E. Ansari, et al. Treatment of open-angle glaucoma and ocular hypertension with preservative-free tafuprost/timolol fxed-dose combination therapy: 6 case reports and clinical outcomes. BMC Ophthalmol. 2022 Apr 2;22(1):152. [Content Brief]

[3]. Figen Amber Cicek, et al. Beta-blocker timolol alleviates hyperglycemia-induced cardiac damage via inhibition of endoplasmic reticulum stress. J Bioenerg Biomembr. 2014 Oct;46(5):377-87. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Timolol hemimaleate

6 Cited Publications

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Timolol hemimaleate

Publications Citing Use of MedChemExpress (MCE) Timolol hemimaleate

All Adrenergic Receptor Isoforms

Biological Activity

Chemical Information

Publications (6)

Purity & Documentation

References

Calculators

Help & FAQs

Timolol hemimaleate Related Classifications

HY-17494B Related Classifications

Bioz