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  4. Tirofiban hydrochloride

Tirofiban hydrochloride  (Synonyms: L700462 hydrochloride; MK383 hydrochloride)

Cat. No.: HY-17369A
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Tirofiban (L700462) hydrochloride is a selective and reversible platelet integrin receptor (Gp IIb/IIIa) antagonist that inhibits fibrinogen binding to this receptor and has antithrombotic activity. Tirofiban hydrochloride induces proliferation and migration on endothelial cell by inducing production of VEGF. Tirofiban hydrochloride can significantly reduces myocardial no-reflow and ischemia-reperfusion injury by alleviating myocardial microvascular structural and endothelial dysfunction in the ischemic area.

For research use only. We do not sell to patients.

CAS No. : 142373-60-2

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Tirofiban hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Tirofiban hydrochloride purchased from MedChemExpress. Usage Cited in: Microsyst Nanoeng. 2026 Jan 1;12(1):7.  [Abstract]

    Anti-aggregation efficiency of single-drug combinations for platelets in the combinatorial screening tests for Volunteer 1. For Aspirin (Asp), the concentrations were 2 μM, 4 μM (2 μM + 2 μM), 20 μM, 22 μM (2 μM + 20 μM), and 40 μM (20 μM + 20 μM). For Tirofiban (Tif), the concentrations were 0.2 μM, 0.4 μM (0.2 μM + 0.2 μM), 2 μM, 2.2 μM (0.2 μM + 2 μM), and 4 μM (2 μM + 2 μM). For Ticagrelor, the concentrations were 1 μM, 2 μM (1 μM + 1 μM), 10 μM, 11 μM (10 μM + 1 μM), and 20 μM (10 μM + 10 μM). The results showed that 4 μM Tif (Tirofiban) exhibited the strongest platelet aggregation inhibition.

    Tirofiban hydrochloride purchased from MedChemExpress. Usage Cited in: Am J Respir Cell Mol Biol. 2025 Feb 28.  [Abstract]

    CCK8 analysis indicated that the proliferation effect of PH-PLT-EC CM on PASMCs was diminished by Tirofiban (30 μg/mL) or eptifibatide (10 μg/mL). MFI = mean fluorescence intensity.

    Tirofiban hydrochloride purchased from MedChemExpress. Usage Cited in: Am J Respir Cell Mol Biol. 2025 Feb 28.  [Abstract]

    Treatment with GPIIb/IIIa antagonists Tirofiban (30 or 300 μg/mL; 45 min) or eptifibatide (1; 10 μg/mL) decreased platelet–EC adhesion. Platelet adhesion was measured by quantifying the remaining fluorescence intensity after the unattached platelets were removed.

    Tirofiban hydrochloride purchased from MedChemExpress. Usage Cited in: Am J Respir Cell Mol Biol. 2025 Feb 28.  [Abstract]

    The conditioned medium from EC–platelet cocultures treated with Tirofiban (30 μg/mL), eptifibatide (10 μg/mL), or A-205804 (250 μmol/L) showed reduced PDGF-BB levels.

    Tirofiban hydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2025 February 01.

    RGD-integrin selectivity of lassotides 36-38 and conjugates 47-49 (shaded), as measured by competitive inhibition vs LAP in ELISA assays against integrins αvβ3, αvβ6 and αvβ8 (as described above) and αvβ1, αvβ5, αIIbβ3, α5β1, and α8β1. *MK-042948 was used as a positive control for all integrin assays except αIIbβ3, where Tirofiban49 was employed.

    View All Integrin Isoform Specific Products:

    View All Isoforms
    αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

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    Description

    Tirofiban (L700462) hydrochloride is a selective and reversible platelet integrin receptor (Gp IIb/IIIa) antagonist that inhibits fibrinogen binding to this receptor and has antithrombotic activity. Tirofiban hydrochloride induces proliferation and migration on endothelial cell by inducing production of VEGF. Tirofiban hydrochloride can significantly reduces myocardial no-reflow and ischemia-reperfusion injury by alleviating myocardial microvascular structural and endothelial dysfunction in the ischemic area[1][2][3].

    IC50 & Target

    Gp IIb/IIIa receptor[1].
    In Vitro

    Tirofiban hydrochloride (0.25, 1, 3 μg/mL; 72 hours) increases proliferation of HAEC cells[1].
    Tirofiban hydrochloride (24 hours) closes the scratch of HUVECs migration within 18 hours[1].
    Tirofiban hydrochloride (0.25, 1 μg/mL; 1 hour) induces production of VEGF after 30 minutes which can stimulates proliferation of endothelial cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tirofiban hydrochloride Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: HAEC cells
    Concentration: 0.25, 1, 3 μg/mL
    Incubation Time: 72 hours
    Result: Increased proliferation of HAEC cells.

    Cell Migration Assay [1]

    Cell Line: HUVEC cells
    Concentration:
    Incubation Time: 24 hours
    Result: Stimulated the migratory capacity of endothelial cells.

    Western Blot Analysis[1]

    Cell Line: HAEC cells
    Concentration: 0.05, 0.12, 0.25, 1 μg/mL
    Incubation Time: 1 hour
    Result: Induced production of VEGF which stimulated proliferation of endothelial cells.
    In Vivo

    Tirofiban hydrochloride (60 μg/kg; i.v.; once) shows activity of increasing contraction force, ventricular compliance, and improving heart function by increasing HR, LVESP, dp/dtmax, and reducing LVEDP[2].
    Tirofiban hydrochloride (60 μg/kg; i.v.; once) enhances eNOS activity, decreases iNOS activity and reduces area of no-reflow after reperfusion following AMI[2].
    Tirofiban hydrochloride (50 µg/per; irrigate; once) shows anticoagulant effect with patency rates of 59% at 24 hours after microvascular anastomosis in the crush model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Sprague-Dawley rats (10 to 15-week-age; 270-330 g)[2].
    Dosage: 60 μg/kg
    Administration: Intravenous injection; once.
    Result: Increased contraction force, ventricular compliance, and improved heart function.
    Reduced the size of no-reflow and infarct.
    Animal Model: Sprague-Dawley rats (350-400 g; crush injury model)[3]
    Dosage: 50 µg/per (50 µg/mL, 1 mL for each)
    Administration: Irrigate 1 mL within the vessel lumen (before placement of the last suture); once.
    Result: Showed anticoagulant effect with patency rates of 59%.
    Molecular Weight

    477.06

    Formula

    C22H37ClN2O5S
    CAS No.
    SMILES

    CCCCS(N[C@H](C(O)=O)CC1=CC=C(C=C1)OCCCCC2CCNCC2)(=O)=O.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Tirofiban hydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tirofiban142373-60-2L700462MK383L 700462L-700462MK 383MK-383IntegrinACSsAMINRHUVECHAECVEGFantithromboticInhibitorinhibitorinhibit

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