Art. -Nr.: HY-117099

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TMB-8

Name: TMB-8
Brand: MedChemExpress
SKU: HY-117099
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TMB-8 is a benzoate ester compound. TMB-8 blocks intracellular calcium (Ca) release, catecholamine secretion, and cholinergic/CCK-stimulated amylase release, while stimulating pancreatic hormone release and, under hyperthermia conditions, increasing cytosolic calcium and hyperthermic cell killing. TMB-8 is used in research on chemotherapy-induced painful peripheral neuropathy, human promyelocytic leukemia, and mouse breast cancer.

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Reinheit : 98.0%
CAS. Nr.: 53464-72-5
Formel: C₂₂H₃₈ClNO₅
Molecular Weight:431.99

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

TMB-8 Chemical Structure

CAS. Nr.: 53464-72-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Anti-cancerCarbohydrate MetabolismCardiovascular RegulationRenal RegulationOthers

Anti-cancer

TMB-8 (10-100 μM; 4 days) inhibits HL-60 cell proliferation only at 100 μM while maintaining viability above 85%^[8].
TMB-8 (10-100 μM) alone induces NBT-positive differentiation of HL-60 cells^[8].

Carbohydrate Metabolism

TMB-8 dose-dependently stimulates the release of SRIF, IRG, and IRI from isolated rat pancreatic islets under 2.5 mM glucose conditions, and stimulates SRIF and IRG release without altering IRI under 10 mM glucose conditions^[5].

Cardiovascular Regulation

Renal Regulation

TMB-8 inhibits angiotensin II- and K⁺-stimulated aldosterone secretion in dispersed bovine adrenal zona glomerulosa cells, with ID₅₀ values of 46 µM and 28 µM, respectively^[10].

Others

TMB-8 concentration-dependently inhibits catecholamine secretion from isolated perfused rat adrenal glands evoked by Ach, excessive K⁺, DMPP, McN-A-343 (HY-107648), BAY-K 8644 (HY-10588), and 1,3,7-Trimethylxanthine, and almost completely blocks it at 100 μM^[3].
TMB-8 inhibits 1 μM Carbachol (Carbamoylcholine chloride) (HY-B1208)-stimulated amylase release, producing 57% inhibition at 10 μM, without affecting basal or 0.1 μM secretin-stimulated release^[4].
TMB-8 non-selectively inhibits parasympathetic neuromuscular transmission in guinea pig ileum and contractions induced by ACh, Nicotine, DMPP, KCl, and BaCl₂, with ID₅₀ values of 3.8 × 10^-6 to 14.4 × 10^-6 M, but BaCl₂ antagonism is competitive^[7].
TMB-8 directly inhibits protein kinase C activity supported by Ca²⁺ and phosphatidylserine in the cytosol of partially purified bovine adrenal zona glomerulosa cells in a dose-dependent manner^[10].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[8]

Cell Line:

HL-60 human promyelocytic leukemia cells
Concentration:

10, 50, and 100 μM
Incubation Time:

4 days
Result:

At less than 50 μM, did not affect HL-60 cell proliferation.
At 100 μM, little cell proliferation was observed, but cell viability was always more than 85%.

In Vivo

TMB-8 (46 nmol; i.t.; single injection) attenuates Paclitaxel (HY-B0015)-induced mechanical hypersensitivity in rats^[1].
TMB-8 (46 nmol; i.t.; single injection) attenuates Vincristine (HY-N0488A)-induced mechanical hypersensitivity in rats^[1].
TMB-8 (46 nmol; i.t.; single injection) attenuates ddC (Zalcitabine) (HY-17392)-induced mechanical hyperalgesia in rats^[1].
TMB-8 (116 nmol/10 µL; intradermal injection; single injection) attenuates ddC-induced mechanical hyperalgesia in rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (male, 250-270 g at start)^[1]
Dosage:

46 nmol in 20 µL
Administration:

i.t.; single injection; at 22-28 days after paclitaxel
Result:

Reduced paclitaxel-evoked mechano-allodynia on the 4-g von Frey test and mechano-hyperalgesia on the 15-g von Frey and paw-pressure tests.

Animal Model:

Sprague-Dawley (male, 250-270 g at start)^[1]
Dosage:

46 nmol in 20 µL
Administration:

i.t.; single injection; at 13-19 days after vincristine
Result:

Reduced vincristine-evoked mechano-allodynia on the 4-g von Frey test and mechano-hyperalgesia on the 15-g von Frey and paw-pressure tests.

Animal Model:

Sprague-Dawley (male, 250-270 g at start)^[1]
Dosage:

46 nmol in 20 µL
Administration:

i.t.; single injection; at 8 days after ddC
Result:

Reduced ddC-evoked mechano-hyperalgesia in the paw-pressure test.

Animal Model:

Sprague-Dawley (male, 250-270 g at start)^[1]
Dosage:

116 nmol/10 µL
Administration:

intradermal; single injection; at 8 days after ddC
Result:

Reduced ddC-evoked mechano-hyperalgesia in the paw-pressure test.

Related Protocols :

Chemical Information

CAS. Nr. 53464-72-5
Appearance Solid
Molecular Weight 431.99
Formel C₂₂H₃₈ClNO₅
Color White to off-white
SMILES

COC1=C(C(OC)=CC(C(OCCCCCCCCN(CC)CC)=O)=C1)OC.Cl
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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iScience

2026 Aug 22;29(9):117190. PMID: 42668535

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (115.74 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3149 mL

11.5743 mL

23.1487 mL

5 mM

0.4630 mL

2.3149 mL

4.6297 mL

10 mM

0.2315 mL

1.1574 mL

2.3149 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Siau C, et al. Dysregulation of cellular calcium homeostasis in chemotherapy-evoked painful peripheral neuropathy. Anesthesia and analgesia. 2006 May;102(5):1485-90. [Content Brief]

[2]. Galeotti N, et al. The phospholipase C-IP3 pathway is involved in muscarinic antinociception. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 2003 May;28(5):888-97.

[3]. Lim DY, et al. Influence of TMB-8 on secretion of catecholamines from the perfused rat adrenal glands. Archives of Pharmacal Research. 1992 Jun;15(2):115-25.

[4]. Ikeda M, et al. Effect of an intracellular calcium antagonist (TMB-8) on carbamylcholine-induced amylase release from dispersed rat pancreatic acini. Life sciences. 1984 Feb 06;34(6):529-33. [Content Brief]

[5]. Yasuda H, et al. Stimulative effects of TMB-8 and trifluoperazine on pancreatic hormone release. Diabetes research and clinical practice. 1989 Apr 01;6(3):227-32. [Content Brief]

[6]. Wojewodzka M, et al. Calcium antagonist, TMB-8, prevents the induction of adaptive response by hydrogen peroxide or X-rays in human lymphocytes. International journal of radiation biology. 1994 Jul;66(1):99-109. [Content Brief]

[7]. Malagodi MH, et al. Pharmacological evaluation of a new Ca2+ antagonist, 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8): studies in smooth muscles. Eur J Pharmacol. 1974 Jun;27(1):25-33. [Content Brief]

[8]. Okazaki T, et al. Role of intracellular calcium ion in human promyelocytic leukemia HL-60 cell differentiation. Cancer research. 1986 Dec;46(12 Pt 1):6059-63. [Content Brief]

[9]. Kondo T, et al. Enhancement of cell killing and increase in cytosolic calcium concentration by combined treatments with hyperthermia and TMB-8 in mouse mammary carcinoma FM3A cells. Cell calcium. 1993 Oct;14(9):621-9. [Content Brief]

[10]. Kojima I, et al. Mechanism of inhibitory action of TMB-8 [8-(NN-diethylamino)octyl-3,4,5-trimethoxybenzoate] on aldosterone secretion in adrenal glomerulosa cells. The Biochemical journal. 1985 Nov 15;232(1):87-92.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3149 mL	11.5743 mL	23.1487 mL	57.8717 mL
	5 mM	0.4630 mL	2.3149 mL	4.6297 mL	11.5743 mL
	10 mM	0.2315 mL	1.1574 mL	2.3149 mL	5.7872 mL
	15 mM	0.1543 mL	0.7716 mL	1.5432 mL	3.8581 mL
	20 mM	0.1157 mL	0.5787 mL	1.1574 mL	2.8936 mL
	25 mM	0.0926 mL	0.4630 mL	0.9259 mL	2.3149 mL
	30 mM	0.0772 mL	0.3858 mL	0.7716 mL	1.9291 mL
	40 mM	0.0579 mL	0.2894 mL	0.5787 mL	1.4468 mL
	50 mM	0.0463 mL	0.2315 mL	0.4630 mL	1.1574 mL
	60 mM	0.0386 mL	0.1929 mL	0.3858 mL	0.9645 mL
	80 mM	0.0289 mL	0.1447 mL	0.2894 mL	0.7234 mL
	100 mM	0.0231 mL	0.1157 mL	0.2315 mL	0.5787 mL

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TMB-8

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