TNIK-IN-5
Based on 1 Customer Validation
TNIK-IN-5 is an efficient TNIK inhibitor with IC50 of 0.05 μM. TNIK-IN-5 efficiently inhibits Wnt signaling in intact cells. TNIK-IN-5 shows excellent in vitro anti-colorectal cancer activity.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 2754265-66-0
- Formula: C22H17N3O3
- Molecular Weight:371.39
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Stem Cell Signaling Compound Library
HY-L017
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Wnt/Hedgehog/Notch Compound Library
HY-L020
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Differentiation Inducing Compound Library
HY-L038
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Cytoskeleton Compound Library
HY-L060
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Neuroprotective Compound Library
HY-L070
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Anti-Breast Cancer Compound Library
HY-L074
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Anti-Liver Cancer Compound Library
HY-L101
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Anti-Colorectal Cancer Compound Library
HY-L103
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Anti-Prostate Cancer Compound Library
HY-L124
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Anti-Pulmonary Fibrosis Compound Library
HY-L125
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Cancer Stem Cells Compound Library
HY-L135
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Heterocyclic Compound Library
HY-L138
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Antihypertensive Compound Library
HY-L145
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Extracellular Vesicles (EVs) Compound Library
HY-L168
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Anti-Gastric Cancer Compound Library
HY-L184
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Anti-Fibrosis Compound Library
HY-L185
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High-Throughput Bioactive Compound Library
HY-L205
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Anti-Rheumatic Arthritis Compound Library
HY-L210
Biological Activity
IC50: 0.05 μM (TNIK)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
4.997 μM
Compound: 8g
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Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
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[PMID: 35447345] |
TNIK-IN-5 (compound 8g) (1.25-5 μM; 48 hours) significantly inhibits the cell proliferation of HCT116 in a dose-dependent manner[1].
TNIK-IN-5 (1 μM; 24, 48 hours) strongly prevents HCT116 cells migration[1].
TNIK-IN-5 (10-40 μM; 48 hours) concentration-dependently inhibits the protein levels of β-catenin and TCF-4 in the nucleus, and significantly decreases the expression of Wnt target gene products, including Axin2 and c-Myc[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 cells[1]
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Concentration:1.25 μM, 2.5 μM, 5 μM
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Incubation Time:48 hours
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Result:Significantly inhibited the cell proliferation in a dose-dependent manner.
Chemical Information
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CAS No. 2754265-66-0
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Appearance Solid
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Molecular Weight 371.39
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Formula C22H17N3O3
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Color White to light yellow
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SMILES
O=C1OC2=CC(C3=CN=C(NC=C4)C4=C3)=CC=C2N1CC5=CC=CC(OC)=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 10 mg/mL (26.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6926 mL | 13.4629 mL | 26.9259 mL | 67.3147 mL |
| 5 mM | 0.5385 mL | 2.6926 mL | 5.3852 mL | 13.4629 mL | |
| 10 mM | 0.2693 mL | 1.3463 mL | 2.6926 mL | 6.7315 mL | |
| 15 mM | 0.1795 mL | 0.8975 mL | 1.7951 mL | 4.4876 mL | |
| 20 mM | 0.1346 mL | 0.6731 mL | 1.3463 mL | 3.3657 mL | |
| 25 mM | 0.1077 mL | 0.5385 mL | 1.0770 mL | 2.6926 mL |
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