trans-Melilotoside
Based on 1 Customer Validation
trans-Melilotoside is a coumarin precursor and that can be isolated from Melilotus neapolitan. trans-Melilotoside exhibits antiparasitic and DPPH radical-scavenging activity. trans-Melilotoside can be used for the research of human african trypanosomiasis, chagas’ disease, malaria[1][3].
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 618-67-7
- Formula: C15H18O8
- Molecular Weight:326.30
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Parasite Isoforms
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against A549 cells
Cytotoxicity against A549 cells
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[PMID: 17027271] |
| B16 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against B16 cells
Cytotoxicity against B16 cells
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[PMID: 17027271] |
| Bel-7402 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against BEL-7402 cells
Cytotoxicity against BEL-7402 cells
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[PMID: 17027271] |
| BGC-823 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against BGC823 cells
Cytotoxicity against BGC823 cells
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[PMID: 17027271] |
| DU-145 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against DU145 cells
Cytotoxicity against DU145 cells
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[PMID: 17027271] |
| HT-29 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against HT29 cells
Cytotoxicity against HT29 cells
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[PMID: 17027271] |
| MCF7 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against MCF7 cells
Cytotoxicity against MCF7 cells
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[PMID: 17027271] |
| MDA-MB-231 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against MDA-MB-231 cells
Cytotoxicity against MDA-MB-231 cells
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[PMID: 17027271] |
| SGC-7901 | IC50 |
1.6 μg/mL
Compound: 10
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Cytotoxicity against SGC7901 cells
Cytotoxicity against SGC7901 cells
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[PMID: 17027271] |
trans-Melilotoside (10-8-10-4 M; 4 days) moderately inhibits germination of Petrorhagia velutina and growth of Petrorhagia velutina and Dactylis hispanica, while showing low stimulation of Phleum subulatum growth, with effects most pronounced at the 10-4 M concentration[1].
trans-Melilotoside (24-96 h) inhibits the growth of Plasmodium falciparum, Trypanosoma brucei rhodesiense, and Trypanosoma cruzi with IC50 values of 48.7, 58.9, and 58.0 mg/mL, respectively, and shows cytotoxicity against L-6 cells with an IC50 of 63.6 mg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 618-67-7
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Appearance Solid
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Molecular Weight 326.30
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Formula C15H18O8
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Color White to off-white
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SMILES
OC[C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC2=C(C=CC=C2)/C=C/C(O)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (306.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Atay I, et al. Evaluation of in vitro antiprotozoal activity of Ajuga laxmannii and its secondary metabolites. Pharm Biol. 2016;54(9):1808-1814. [Content Brief]
[3]. Yang L, et al. Bio-guided isolation of antioxidants from the stems of Dendrobium aurantiacum var. denneanum. Phytother Res. 2007;21(7):696-698. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0647 mL | 15.3233 mL | 30.6466 mL | 76.6166 mL |
| 5 mM | 0.6129 mL | 3.0647 mL | 6.1293 mL | 15.3233 mL | |
| 10 mM | 0.3065 mL | 1.5323 mL | 3.0647 mL | 7.6617 mL | |
| 15 mM | 0.2043 mL | 1.0216 mL | 2.0431 mL | 5.1078 mL | |
| 20 mM | 0.1532 mL | 0.7662 mL | 1.5323 mL | 3.8308 mL | |
| 25 mM | 0.1226 mL | 0.6129 mL | 1.2259 mL | 3.0647 mL | |
| 30 mM | 0.1022 mL | 0.5108 mL | 1.0216 mL | 2.5539 mL | |
| 40 mM | 0.0766 mL | 0.3831 mL | 0.7662 mL | 1.9154 mL | |
| 50 mM | 0.0613 mL | 0.3065 mL | 0.6129 mL | 1.5323 mL | |
| 60 mM | 0.0511 mL | 0.2554 mL | 0.5108 mL | 1.2769 mL | |
| 80 mM | 0.0383 mL | 0.1915 mL | 0.3831 mL | 0.9577 mL | |
| 100 mM | 0.0306 mL | 0.1532 mL | 0.3065 mL | 0.7662 mL |
trans-Melilotoside Related Classifications
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