Cat. No.: HY-N0017

Bergenin

(Synonyms: Cuscutin)

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Bergenin is a cytoprotective and antioxidative polyphenol found in many medicinal plants. Bergenin has a wide spectrum activities such as hepatoprotective, antiinflammatory, immunomodulatory, antitumor, antiviral, and antifungal properties.

For research use only. We do not sell to patients.

Purity: 99.34%
CAS No.: 477-90-7
Formula: C₁₄H₁₆O₉
Molecular Weight:328.27

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Bergenin Chemical Structure

CAS No.: 477-90-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	77.28 μM Compound: Bergenin	Antiproliferative activity against human A549 cells incubated for 48 to 72 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 28395218]
C8166	EC₅₀	40 μg/mL Compound: 6	Antiviral activity against HIV1 MN in human C8166 cells assessed as reduction of antigen gp120 production by ELISA Antiviral activity against HIV1 MN in human C8166 cells assessed as reduction of antigen gp120 production by ELISA	[PMID: 8786362]
HepG2	IC₅₀	34.56 μM Compound: Bergenin	Antiproliferative activity against human HepG2 cells incubated for 48 to 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 28395218]
MCF7	IC₅₀	>100 μM Compound: Bergenin	Antiproliferative activity against human MCF7 cells incubated for 48 to 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 28395218]
Neutrophil	IC₅₀	303.12 μM Compound: 16	Antiinflammatory activity in human neutrophils assessed as inhibition of opsonized zymosan A-mediated superoxide anions production by WST-1 based respiratory burst assay Antiinflammatory activity in human neutrophils assessed as inhibition of opsonized zymosan A-mediated superoxide anions production by WST-1 based respiratory burst assay	[PMID: 22437110]
PC-3	IC₅₀	>100 μM Compound: Bergenin	Antiproliferative activity against human PC3 cells incubated for 48 to 72 hrs by MTT assay Antiproliferative activity against human PC3 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 28395218]
SGC-7901	IC₅₀	23.56 μM Compound: Bergenin	Antiproliferative activity against human SGC7901 cells incubated for 48 to 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells incubated for 48 to 72 hrs by MTT assay	[PMID: 28395218]
THP-1	IC₅₀	0.208 μM Compound: 1	Inhibition of LPS-induced IL6 production in human THP1 cells after 24 hrs Inhibition of LPS-induced IL6 production in human THP1 cells after 24 hrs	[PMID: 25111439]
THP-1	IC₅₀	12.5 μM Compound: 1	Inhibition of LPS-induced TNFalpha production in human THP1 cells after 24 hrs Inhibition of LPS-induced TNFalpha production in human THP1 cells after 24 hrs	[PMID: 25111439]
THP-1	IC₅₀	130 μM Compound: 1	Cytotoxicity against human THP1 cells after 24 hrs by MTT assay Cytotoxicity against human THP1 cells after 24 hrs by MTT assay	[PMID: 25111439]
THP-1	IC₅₀	84 μM Compound: 1	Cytotoxicity against human THP1 cells after 48 hrs by MTT assay Cytotoxicity against human THP1 cells after 48 hrs by MTT assay	[PMID: 25111439]

In Vitro

Bergenin (7.5-30μM; 24 hours) decreases the viability of HeLa cervical cancer cells (IC₅₀=15 μM)^[1].
Bergenin (7.5-30 μM; 24 hours) induces apoptosis in HeLa cervical cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HeLa cervical cancer cells
Concentration:

7.5, 15, 30 µM
Incubation Time:

24 hours
Result:

Decreased the viability of HeLa cervical cancer cells.

Cell Viability Assay^[1]

Cell Line:

HeLa cervical cancer cells
Concentration:

7.5, 15, 30 µM
Incubation Time:

24 hours
Result:

Induced apoptosis in HeLa cervical cancer cells.

In Vivo

Pretreatment with Bergenin (12.5-100 mg/kg; i.p.; once) produces a dose-related inhibition of acetic acid-induced writhing in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Swiss Webster C57BL/6 mice^[3]
Dosage:

12.5, 25, 50, 100 mg/kg
Administration:

i.p.; Once
Result:

Produced a dose-related inhibition of acetic acid-induced writhing in mice.

Chemical Information

CAS No. 477-90-7
Appearance Solid
Molecular Weight 328.27
Formula C₁₄H₁₆O₉
Color White to off-white
SMILES

OC1=C(OC)C(O)=C([C@@]2([H])[C@@](OC3=O)([H])[C@@H](O)[C@H](O)[C@@H](CO)O2)C3=C1
Synonyms

Cuscutin
Structure Classification
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

All RT-PCRWBApoptosis AnalysisIFIn Vivo Efficacy Study

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Journal Impact Factor

J Transl Med

3D biological scaffold delivers Bergenin to reduce neuroinflammation in rats with cerebral hemorrhage. [Abstract]

2024 Oct 17;22(1):946. PMID: 39420402

Bergenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Oct 17;22(1):946. [Abstract]

Measurement of HIF-1α mRNA expression levels teated with Bergenin (20 μM).

Bergenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Oct 17;22(1):946. [Abstract]

Measurement of HIF-1α protein expression levels teated with Bergenin (20 μM).

Bergenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Oct 17;22(1):946. [Abstract]

Treatment with either Bergenin (20 μM) or NAC resulted in a decrease in Hemin-induced apoptosis.

Bergenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Oct 17;22(1):946. [Abstract]

JC-1 show that 3D biological scaffold encapsulation of Bergenin (20 μM) or NAC treatment can reduce the apoptosis rate induced by Hemin treatment.

Bergenin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Oct 17;22(1):946. [Abstract]

Representative images of hemorrhagic lesions results in rats treated with Bergenin (0.5 μL/kg).
ACS Omega

Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer. [Abstract]

2024 Feb 28;9(10):11870-11882. PMID: 38496939
Sci Rep

Bergenin protects against osteoarthritis by inhibiting STAT3, NF-κB and Jun pathways and suppressing osteoclastogenesis. [Abstract]

2024 Aug 31;14(1):20292. PMID: 39217193
Sci Rep

Bergenin inhibits growth of human cervical cancer cells by decreasing Galectin-3 and MMP-9 expression. [Abstract]

2024 Jul 3;14(1):15287. PMID: 38961106
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Int J Rheum Dis

Antimigratory, Anti-Invasive and Anti-Inflammatory Effects of Bergenin in Rheumatoid Arthritis by Downregulating the Wnt/β-Catenin Pathway. [Abstract]

2025 Jul;28(7):e70360. PMID: 40620076
SSRN

2026 Apr 14.

Solvent & Solubility

In Vitro:

DMSO : 103.3 mg/mL (314.68 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0463 mL

15.2314 mL

30.4627 mL

5 mM

0.6093 mL

3.0463 mL

6.0925 mL

10 mM

0.3046 mL

1.5231 mL

3.0463 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shi X, et al. Anticancer activity of bergenin against cervical cancer cells involves apoptosis, cell cycle arrest, inhibition of cell migration and the STAT3 signalling pathway. Exp Ther Med. 2019 May;17(5):3525-3529. [Content Brief]

[2]. Yun J, et al. Bergenin decreases the morphine-induced physical dependence via antioxidative activity in mice. Arch Pharm Res. 2015 Jun;38(6):1248-54. [Content Brief]

[3]. de Oliveira CM, et al. Antinociceptive properties of bergenin. J Nat Prod. 2011 Oct 28;74(10):2062-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0463 mL	15.2314 mL	30.4627 mL	76.1568 mL
	5 mM	0.6093 mL	3.0463 mL	6.0925 mL	15.2314 mL
	10 mM	0.3046 mL	1.5231 mL	3.0463 mL	7.6157 mL
	15 mM	0.2031 mL	1.0154 mL	2.0308 mL	5.0771 mL
	20 mM	0.1523 mL	0.7616 mL	1.5231 mL	3.8078 mL
	25 mM	0.1219 mL	0.6093 mL	1.2185 mL	3.0463 mL
	30 mM	0.1015 mL	0.5077 mL	1.0154 mL	2.5386 mL
	40 mM	0.0762 mL	0.3808 mL	0.7616 mL	1.9039 mL
	50 mM	0.0609 mL	0.3046 mL	0.6093 mL	1.5231 mL
	60 mM	0.0508 mL	0.2539 mL	0.5077 mL	1.2693 mL
	80 mM	0.0381 mL	0.1904 mL	0.3808 mL	0.9520 mL
	100 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7616 mL

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Bergenin

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Publications Citing Use of MedChemExpress (MCE) Bergenin

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bergenin Related Classifications

HY-N0017 Related Classifications