BL-918
Based on 4 publication(s) in Google Scholar
BL-918 is an orally active UNC-51-like kinase 1 (ULK1) activator with an EC50 of 24.14 nM. BL-918 exerts its cytoprotective autophagic effect by targeting ULK complex. BL-918 has the potential for Parkinson’s disease (PD) treatment.
For research use only. We do not sell to patients.
- Purity: 99.72%
- CAS No.: 2101517-69-3
- Formula: C23H15F8N3OS
- Molecular Weight:533.44
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Autophagy Compound Library
HY-L029
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Orally Active Compound Library
HY-L061
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Anti-Parkinson's Disease Compound Library
HY-L085
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Neurodegenerative Disease-related Compound Library
HY-L086
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Targeted Diversity Library
HY-L099
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Multi-Target Compound Library
HY-L176
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Mitophagy Compound Library
HY-L180
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) BL-918
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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RT-PCR
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WB
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Cell Imaging/Staining
Biological Activity
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ULK1 24.14 nM (EC50) |
ULK1 0.719 μM (Kd) |
BL-918 (compound 33i) binds to ULK1 with a high binding affinity (KD=0.719 μM)[1].
BL-918 (5 μM; for 24 hours) induces autophagy in Neuron-Like SH-SY5Y cells[1].
BL-918 (0.5-50 μM; for 24 hours) can partially reverse MPP+-induced cell death, which is determined by enhancing cell viability[1].
BL-918 (5 μM; for 6-36 hours) time-dependently elevates the expression levels of LC3-II, Beclin-1, and its phosphorylation status, whereas reduces the level of the selective autophagy substrate SQSTM1/p62. BL-918 elevates Ser317 and Ser555 phosphorylation of ULK1, as well as decreases Ser757 phosphorylation of ULK1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SH-SY5Y cells
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Concentration:5 μM
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Incubation Time:For 24 hours
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Result:Induced Autophagy.
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Cell Line:SH-SY5Y cells
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Concentration:0.5, 5, 50 μM
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Incubation Time:For 24 hours
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Result:Could partially reverse MPP+-induced cell death, which was determined by enhancing cell viability.
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Cell Line:SH-SY5Y cells
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Concentration:5 μM
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Incubation Time:6, 12, 24, 36 hours
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Result:Time-dependently elevated the expression levels of LC3-II (a key marker of autophagy), Beclin-1, and its phosphorylation status, whereas reduced the level of the selective autophagy substrate SQSTM1/p62.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6 mice (eight-week old) weighing between 20 and 25 g[1]
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Dosage:20, 40, or 80 mg/kg
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Administration:Oral gavage; daily; began 2 days before the first injection of saline/MPTP and continuously maintained for 5 days after the last injection of saline/MPTP
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Result:Attenuated the loss of DA and its metabolites.
Chemical Information
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CAS No. 2101517-69-3
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Appearance Solid
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Molecular Weight 533.44
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Formula C23H15F8N3OS
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Color White to off-white
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SMILES
O=C(NC1=CC=C(F)C=C1F)[C@H](NC(NC2=CC(C(F)(F)F)=CC(C(F)(F)F)=C2)=S)C3=CC=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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J Exp Clin Cancer Res
Ferrodoxin 1 (FDX1) drives paclitaxel resistance in ovarian cancer via copper metabolism and ULK1/ATG13-mediated autophagy: overcome by pH/ROS-responsive PPD/PDP@si-FDX1 nanomicelles. [Abstract]2026 Apr 23;45(1):104. PMID: 42026685 -
Stem Cell Res Ther
2025 Apr 24;16(1):206. PMID: 40275329
BL-918 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Apr 24;16(1):206. [Abstract]
Western blot analysis of AMPK-ULK1 and autophagic activation in MSCs after osteogenic differentiation for 72 h treated with ULK1 inhibitor SBI-0206965 (SBI, 1 µM) or agonist BL-918 (BL, 10 µM) under control and nZnO (5 µg/mL) conditions.
BL-918 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Apr 24;16(1):206. [Abstract]
ALP staining and ARS staining of MSCs after osteogenic differentiation for 7 days and 14 days treated with ULK1 inhibitor SBI or agonist BL-918 (BL) under control and nZnO (5 µg/mL) conditions, respectively.
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Cell Biol Toxicol
HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicity. [Abstract]2026 Feb 5;42(1):34. PMID: 41639490
BL-918 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2026 Feb 5;42(1):34. [Abstract]
SH-SY5Y cells, transfected with negative control or HUWE1 siRNA, were treated with 50 μM 6-OHDA or 1 mM MPP⁺ for 24 h in the presence or absence of 20 μM BL-918. Cell viability measured by CCK-8 assay.
BL-918 purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2026 Feb 5;42(1):34. [Abstract]
SH-SY5Y cells, transfected with control or HUWE1 siRNA, were treated with 50 μM 6-OHDA or 1 mM MPP⁺ for 24 h in the presence of 20 μM BL-918. qRT-PCR analysis of HUWE1, SIRT1, PPARGC1A, NFE2L2, and TFAM mRNA expression.
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Aquac Nutr
Benfotiamine, a Lipid-Soluble Derivative of Vitamin B1, Ameliorates the Carbohydrate Overload-Induced Mitochondrial Dysfunction in Fish Megalobrama amblycephala by Triggering the ULK1-Mediated Mitophagy. [Abstract]2025 May 1:2025:7688386. PMID: 40343082
Solvent & Solubility
DMSO : ≥ 250 mg/mL (468.66 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8746 mL | 9.3731 mL | 18.7463 mL | 46.8656 mL |
| 5 mM | 0.3749 mL | 1.8746 mL | 3.7493 mL | 9.3731 mL | |
| 10 mM | 0.1875 mL | 0.9373 mL | 1.8746 mL | 4.6866 mL | |
| 15 mM | 0.1250 mL | 0.6249 mL | 1.2498 mL | 3.1244 mL | |
| 20 mM | 0.0937 mL | 0.4687 mL | 0.9373 mL | 2.3433 mL | |
| 25 mM | 0.0750 mL | 0.3749 mL | 0.7499 mL | 1.8746 mL | |
| 30 mM | 0.0625 mL | 0.3124 mL | 0.6249 mL | 1.5622 mL | |
| 40 mM | 0.0469 mL | 0.2343 mL | 0.4687 mL | 1.1716 mL | |
| 50 mM | 0.0375 mL | 0.1875 mL | 0.3749 mL | 0.9373 mL | |
| 60 mM | 0.0312 mL | 0.1562 mL | 0.3124 mL | 0.7811 mL | |
| 80 mM | 0.0234 mL | 0.1172 mL | 0.2343 mL | 0.5858 mL | |
| 100 mM | 0.0187 mL | 0.0937 mL | 0.1875 mL | 0.4687 mL |
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