Vitexin

Name: Vitexin
Brand: MedChemExpress
SKU: HY-N0013
Rating: 5.0 (12 reviews)

Cat. No.: HY-N0013 Purity: 99.48%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Vitexin is a c-glycosylated flavone, and is found in various medicinal plants species such as Trigonella foenum-graecum Linn. Vitexin has a wide range of pharmacological effects, including anti-oxidant, anti-cancer, anti-inflammatory, anti-hyperalgesic, and neuroprotective effects.

For research use only. We do not sell to patients.

CAS No. : 3681-93-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Vitexin:

Vitexin (Standard) In-stock
Vitexin arginine Get quote

12 Publications Citing Use of MCE Vitexin

Cell Imaging/Staining

IHC

RT-PCR

Cell Proliferation/Viability Assay

: Vitexin purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Oct 27;29(1):147. [Abstract]; Vitexin (30 mg/kg/day, i.g.)reduced UUO-induced renal tubular epithelial cell death, Representative images of TUNEL staining in kidney tissues.

: Vitexin purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Oct 27;29(1):147. [Abstract]; The protein levels of cleaved caspase3 in the kidneys of UUO mice with or without Vitexin (30 mg/kg/day, i.g.) treatment were examined by WB.

: Vitexin purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Oct 27;29(1):147. [Abstract]; Representative IHC staining of F4/80 in the kidneys of UUO mice treated with or without Vitexin (30 mg/kg/day, i.g.).

: Vitexin purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Oct 27;29(1):147. [Abstract]; qRT‒PCR revealed that the mRNA levels of IL-1β, IL-6, TNF-α and MCP-1 were highly increased in the UUO group, while Vitexin (30 mg/kg/day, i.g.) treatment abrogated the increase in these inflammatory cytokines.

: Vitexin purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Oct 27;29(1):147. [Abstract]; Viability of HK2 cells treated with Vitexin (25, 50, 100, 200, 400 μM).

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	EC₅₀	> 86.2069 μM Compound: 1	Antiproliferative activity against human BJ cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human BJ cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
HepG2	EC₅₀	> 43.29 μM Compound: 1	Antiproliferative activity against human HepG2 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human HepG2 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
KOPN-8	EC₅₀	> 43.29 μM Compound: 1	Antiproliferative activity against human KOPN8 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human KOPN8 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
SEM	EC₅₀	> 43.29 μM Compound: 1	Antiproliferative activity against human SEM cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human SEM cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
SUP-B15	EC₅₀	50.69 μM Compound: 1	Antiproliferative activity against human SUP-B15 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human SUP-B15 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
THLE-2	EC₅₀	200 μM Compound: 1	Antiproliferative activity against human THLE2 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human THLE2 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
UoC-B1	EC₅₀	> 43.29 μM Compound: 1	Antiproliferative activity against human UOCB1 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human UOCB1 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]

In Vivo

Vitexin exerts neuroprotective effect against pentylenetetrazole-induced seizures, scopolamine-induced memory impairment, and glutamate-induced neuronal excitotoxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

432.38

Formula

C₂₁H₂₀O₁₀

CAS No.

3681-93-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

OC1=CC=C(C2=CC(C(C(O)=CC(O)=C3[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O4)=C3O2)=O)C=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (115.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 4 mg/mL (9.25 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3128 mL	11.5639 mL	23.1278 mL
5 mM	0.4626 mL	2.3128 mL	4.6256 mL
10 mM	0.2313 mL	1.1564 mL	2.3128 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.48%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (256 KB) HNMR (273 KB) RP-HPLC (250 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. He M, et al. A review on the pharmacological effects of vitexin and isovitexin. Fitoterapia. 2016 Dec;115:74-85. [Content Brief]

[2]. Lima LKF, et al. A Brief Review on the Neuroprotective Mechanisms of Vitexin. Biomed Res Int. 2018 Dec 5;2018:4785089. [Content Brief]

[3]. Jiang J, Dai J, Cui H. Vitexin reverses the autophagy dysfunction to attenuate MCAO-induced cerebral ischemic stroke via mTOR/Ulk1 pathway. Biomed Pharmacother. 2018;99:583-590. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF / DMSO	1 mM	2.3128 mL	11.5639 mL	23.1278 mL	57.8195 mL
DMF / DMSO	5 mM	0.4626 mL	2.3128 mL	4.6256 mL	11.5639 mL
DMSO	10 mM	0.2313 mL	1.1564 mL	2.3128 mL	5.7820 mL
	15 mM	0.1542 mL	0.7709 mL	1.5419 mL	3.8546 mL
	20 mM	0.1156 mL	0.5782 mL	1.1564 mL	2.8910 mL
	25 mM	0.0925 mL	0.4626 mL	0.9251 mL	2.3128 mL
	30 mM	0.0771 mL	0.3855 mL	0.7709 mL	1.9273 mL
	40 mM	0.0578 mL	0.2891 mL	0.5782 mL	1.4455 mL
	50 mM	0.0463 mL	0.2313 mL	0.4626 mL	1.1564 mL
	60 mM	0.0385 mL	0.1927 mL	0.3855 mL	0.9637 mL
	80 mM	0.0289 mL	0.1445 mL	0.2891 mL	0.7227 mL
	100 mM	0.0231 mL	0.1156 mL	0.2313 mL	0.5782 mL

Vitexin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vitexin3681-93-4AutophagyMedicinalplantsanti-canceranti-oxidantantiinflammatoryanti-nociceptiveanti-hypertensiveanti-spasmodicanti-viral neurodegenerationInhibitorinhibitorinhibit

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Vitexin

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Vitexin Related Antibodies

12 Publications Citing Use of MCE Vitexin

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Complete Stock Solution Preparation Table

Vitexin Related Classifications

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