β-Thujaplicinol
Based on 1 Customer Validation
β-Thujaplicinol (3-Hydroxy-5-isopropyltropolone) is an inhibitor of hepatitis B virus (HBV) ribonuclease H. β-Thujaplicinol inhibits RNAseHs of HBV genotypes D and H with IC50 values of 5.9 and 2.3 μM, respectively.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 29346-20-1
- Formula: C10H12O3
- Molecular Weight:180.20
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CEM-SS | CC50 |
2.3 μM
Compound: beta-thujaplicinol
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Cytotoxicity against human CEM-SS cells
Cytotoxicity against human CEM-SS cells
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[PMID: 21568335] |
| CEM-SS | IC50 |
2.3 μM
Compound: beta-thujaplicinol
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Cytotoxicity against human CEM-SS cells assessed as cell growth inhibition
Cytotoxicity against human CEM-SS cells assessed as cell growth inhibition
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[PMID: 25587934] |
| HepG2 | CC50 |
47 μM
Compound: 32
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTS assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTS assay
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[PMID: 37216809] |
| HepG2 | CC50 |
48 μM
Compound: 32
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability in presence of lamivudine by MTS assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability in presence of lamivudine by MTS assay
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[PMID: 37216809] |
| MT4 | CC50 |
2.5 μM
Compound: 1
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Cytotoxicity against human MT4 cells assessed as reduction in cell viability measured after 5 days by MTT assay
Cytotoxicity against human MT4 cells assessed as reduction in cell viability measured after 5 days by MTT assay
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[PMID: 35780636] |
| MT4 | CC50 |
2.83 μM
Compound: Fig 1, Cpd 2
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Cytotoxicity in mock-infected human MT4 cells assessed as reduction in cell viability incubated for 5 days by MTT assay
Cytotoxicity in mock-infected human MT4 cells assessed as reduction in cell viability incubated for 5 days by MTT assay
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[PMID: 31324562] |
| Vero | CC50 |
>100 μM
Compound: beta-thujaplicinol
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Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by MTT assay
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[PMID: 31391890] |
| Vero | EC50 |
1.7 μM
Compound: beta-thujaplicinol
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Inhibition of NTS in HSV-2 infected in African green monkey Vero cells assessed as suppression of viral replication by measuring viral titer after 24 hrs by plaque reduction assay based phase contrast microscopy
Inhibition of NTS in HSV-2 infected in African green monkey Vero cells assessed as suppression of viral replication by measuring viral titer after 24 hrs by plaque reduction assay based phase contrast microscopy
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[PMID: 31391890] |
| Vero | EC50 |
2 μM
Compound: beta-thujaplicinol
|
Inhibition of NTS in HSV-1 infected in African green monkey Vero cells assessed as suppression of viral replication by measuring viral titer after 24 hrs by plaque reduction assay based phase contrast microscopy
Inhibition of NTS in HSV-1 infected in African green monkey Vero cells assessed as suppression of viral replication by measuring viral titer after 24 hrs by plaque reduction assay based phase contrast microscopy
|
[PMID: 31391890] |
Chemical Information
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CAS No. 29346-20-1
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Appearance Solid
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Molecular Weight 180.20
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Formula C10H12O3
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Color Off-white to light brown
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SMILES
CC(C1=CC(C(O)=C(C=C1)O)=O)C
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Synonyms
3-Hydroxy-5-isopropyltropolone
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (554.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.5494 mL | 27.7469 mL | 55.4939 mL | 138.7347 mL |
| 5 mM | 1.1099 mL | 5.5494 mL | 11.0988 mL | 27.7469 mL | |
| 10 mM | 0.5549 mL | 2.7747 mL | 5.5494 mL | 13.8735 mL | |
| 15 mM | 0.3700 mL | 1.8498 mL | 3.6996 mL | 9.2490 mL | |
| 20 mM | 0.2775 mL | 1.3873 mL | 2.7747 mL | 6.9367 mL | |
| 25 mM | 0.2220 mL | 1.1099 mL | 2.2198 mL | 5.5494 mL | |
| 30 mM | 0.1850 mL | 0.9249 mL | 1.8498 mL | 4.6245 mL | |
| 40 mM | 0.1387 mL | 0.6937 mL | 1.3873 mL | 3.4684 mL | |
| 50 mM | 0.1110 mL | 0.5549 mL | 1.1099 mL | 2.7747 mL | |
| 60 mM | 0.0925 mL | 0.4624 mL | 0.9249 mL | 2.3122 mL | |
| 80 mM | 0.0694 mL | 0.3468 mL | 0.6937 mL | 1.7342 mL | |
| 100 mM | 0.0555 mL | 0.2775 mL | 0.5549 mL | 1.3873 mL |
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