Cat. No.: HY-W004049

3-Hydroxybenzoic acid

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3-Hydroxybenzoic acid is an orally active endogenous metabolite. 3-Hydroxybenzoic acid is an agonist for GPR81 and GPR109A. 3-Hydroxybenzoic acid acts as stress response desensitizers. 3-Hydroxybenzoic acid can be used for anti-inflammatory and analgesic study.

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Pureté: 99.95%
CAS No.: 99-06-9
Formule: C₇H₆O₃
Masse moléculaire:138.12

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

3-Hydroxybenzoic acid Chemical Structure

CAS No.: 99-06-9

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

Voir tous les produits spécifiques à Isoform Endogenous Metabolite :

Voir tous les produits spécifiques à Isoform Hydroxycarboxylic Acid Receptor (HCAR) :

HCAR1
HCAR2

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	>100 μM Compound: b	Inhibition of human recombinant PTP1B expressed in CHO cells assessed as p-nitorphenol production after 2 mins by microplate reader Inhibition of human recombinant PTP1B expressed in CHO cells assessed as p-nitorphenol production after 2 mins by microplate reader	[PMID: 21745700]
COS-7	EC₅₀	184 μM Compound: 1	Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR81 transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]
COS-7	EC₅₀	215 μM Compound: 1	Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]

In Vitro

3-Hydroxybenzoic acid (0.01-10⁵ μM) acts as an agonist for Hydroxycarboxylic acid receptor 1 (HCA1) (GPR81) and HCA2 (GPR109A)^[1].
3-Hydroxybenzoic acid (0.1-10⁵ μM) inhibits cAMP accumulation in SK-N-MC cells which stably expressing human HCA1^[1].
3-Hydroxybenzoic acid (100-1000 μM, 2 h) inhibits free fatty acid and glycerol release in mature adipocytes isolated from mouse subcutaneous and epididymal fat^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

3-Hydroxybenzoic acid protects mice (3-300 mg/kg; p.o.; 7 times in 12 days) and rats (20 mg/kg; p.o.; 7 times in 12 days) against stress triggered alterations in body weights and core temperatures, acts as stress response desensitizers^[2].
3-Hydroxybenzoic acid (20 mg/kg; p.o; 9 times in 12 days) possesses anti-inflammatory and analgesic activities in wistar rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male Wistar rats (150 g)^[2]
Dosage:

20 mg/kg
Administration:

Oral gavage (p.o.); 7 times in 12 days
Result:

Could counteract mild unpredictable stress triggered body weight losses and slight elevation of basal rectal temperatures in rats.
Decreased stress induced hyperthermia.
Significantly lower mean plasma glucose and cortisol and higher insulin levels were observed.

Animal Model:

Male Swiss mice (20 g) with occasional foot shock stress^[2]
Dosage:

3, 10, 30, 100, 300 mg/kg
Administration:

Oral gavage (p.o.); 7 times in 12 days
Result:

Remained constant up to the 10th day of the experiment, but after 30 mg/kg or more daily doses mean body weights of the treated groups increased dose dependently on the last observational days.
Intermittent foot-shock induced elevations of core temperatures were antagonized significantly.
Decreased stress induced hyperthermia.
Had statistically significant effects on immobility period in tail suspension test on male mice.
Statistically significant prolongation of pentobarbitone induced sleep duration was observed after 30 mg/kg and higher daily doses.

Chemical Information

CAS No. 99-06-9
Appearance Solid
Masse moléculaire 138.12
Formule C₇H₆O₃
Color White to off-white
SMILES

OC(C1=CC(O)=CC=C1)=O
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (724.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.2401 mL

36.2004 mL

72.4008 mL

5 mM

1.4480 mL

7.2401 mL

14.4802 mL

10 mM

0.7240 mL

3.6200 mL

7.2401 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (18.10 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (18.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Liu C, et al. 3,5-Dihydroxybenzoic acid, a specific agonist for hydroxycarboxylic acid 1, inhibits lipolysis in adipocytes. J Pharmacol Exp Ther. 2012 Jun;341(3):794-801. [Content Brief]

[2]. Saba Anjum Khan et al. Potential Anti-Stress, Anxiolytic and Antidepressant Like Activities of Mono-Hydroxybenzoic Acids and Aspirin in Rodents: A Comparative Study. Austin J Pharmacol Ther. Volume 3 Issue 3-2015.

[3]. Khan SA, Chatterjee SS, Kumar V. Low dose aspirin like analgesic and anti-inflammatory activities of mono-hydroxybenzoic acids in stressed rodents. Life Sci. 2016 Mar 1;148:53-62. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	7.2401 mL	36.2004 mL	72.4008 mL	181.0020 mL
	5 mM	1.4480 mL	7.2401 mL	14.4802 mL	36.2004 mL
	10 mM	0.7240 mL	3.6200 mL	7.2401 mL	18.1002 mL
	15 mM	0.4827 mL	2.4134 mL	4.8267 mL	12.0668 mL
	20 mM	0.3620 mL	1.8100 mL	3.6200 mL	9.0501 mL
	25 mM	0.2896 mL	1.4480 mL	2.8960 mL	7.2401 mL
	30 mM	0.2413 mL	1.2067 mL	2.4134 mL	6.0334 mL
	40 mM	0.1810 mL	0.9050 mL	1.8100 mL	4.5251 mL
	50 mM	0.1448 mL	0.7240 mL	1.4480 mL	3.6200 mL
	60 mM	0.1207 mL	0.6033 mL	1.2067 mL	3.0167 mL
	80 mM	0.0905 mL	0.4525 mL	0.9050 mL	2.2625 mL
	100 mM	0.0724 mL	0.3620 mL	0.7240 mL	1.8100 mL

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3-Hydroxybenzoic acid

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Voir tous les produits spécifiques à Isoform Endogenous Metabolite

Voir tous les produits spécifiques à Isoform Hydroxycarboxylic Acid Receptor (HCAR)

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

3-Hydroxybenzoic acid Related Classifications

HY-W004049 Related Classifications