2. TGF-beta/Smad Stem Cell/Wnt Metabolic Enzyme/Protease
  3. PKA Phosphodiesterase (PDE)
  4. 8-CPT-Cyclic AMP sodium

8-CPT-Cyclic AMP sodium  (Synonyms: 8-CPT-cAMP sodium; 8-(p-Chlorophenylthio)-cAMP sodium)

Cat. No.: HY-111673 Purity: 99.92%
COA Handling Instructions

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8-CPT-Cyclic AMP (8-CPT-cAMP) sodium is a selective activator of cyclic AMP-dependent protein kinase (PKA). 8-CPT-Cyclic AMP sodium is also a potent inhibitor of the cyclic GMP-specific phosphodiesterase (PDE VA) with an IC50 of 0.9 μM. 8-CPT-Cyclic AMP sodium also inhibits PDE III and PDE IV with IC50Epac and is a potent Epac activator.

For research use only. We do not sell to patients.

8-CPT-Cyclic AMP sodium Chemical Structure

8-CPT-Cyclic AMP sodium Chemical Structure

CAS No. : 93882-12-3

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5 mg USD 110 In-stock
10 mg USD 180 In-stock
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Based on 1 publication(s) in Google Scholar

8-CPT-Cyclic AMP sodium Related Antibodies

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

  • Biological Activity

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Description

8-CPT-Cyclic AMP (8-CPT-cAMP) sodium is a selective activator of cyclic AMP-dependent protein kinase (PKA). 8-CPT-Cyclic AMP sodium is also a potent inhibitor of the cyclic GMP-specific phosphodiesterase (PDE VA) with an IC50 of 0.9 μM. 8-CPT-Cyclic AMP sodium also inhibits PDE III and PDE IV with IC50Epac and is a potent Epac activator[1][2].

In Vitro

a-Fas and TNF-α/CHX induces neutrophil death rapidly (within 2 h) to at least 90%. The commonly used cAMP analog 8-pCPT-cAMP (0.7 mM) delays TNF- /CHX-induced and a-Fas-induced apoptosis. It is more efficient against apoptosis induced by TNF- /CHX than against a-Fas[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8-CPT-Cyclic AMP sodium Related Antibodies
Molecular Weight

493.79

Formula

C16H14ClN5NaO6PS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC=NC2=C1N=C(N2[C@@H]3O[C@@]4([H])[C@](OP(OC4)(O[Na])=O)([H])[C@H]3O)SC5=CC=C(Cl)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (126.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (101.26 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0252 mL 10.1258 mL 20.2515 mL
5 mM 0.4050 mL 2.0252 mL 4.0503 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.92%

COA (255 KB) HNMR (279 KB) CNMR (327 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.0252 mL 10.1258 mL 20.2515 mL 50.6288 mL
5 mM 0.4050 mL 2.0252 mL 4.0503 mL 10.1258 mL
10 mM 0.2025 mL 1.0126 mL 2.0252 mL 5.0629 mL
15 mM 0.1350 mL 0.6751 mL 1.3501 mL 3.3753 mL
20 mM 0.1013 mL 0.5063 mL 1.0126 mL 2.5314 mL
25 mM 0.0810 mL 0.4050 mL 0.8101 mL 2.0252 mL
30 mM 0.0675 mL 0.3375 mL 0.6751 mL 1.6876 mL
40 mM 0.0506 mL 0.2531 mL 0.5063 mL 1.2657 mL
50 mM 0.0405 mL 0.2025 mL 0.4050 mL 1.0126 mL
60 mM 0.0338 mL 0.1688 mL 0.3375 mL 0.8438 mL
80 mM 0.0253 mL 0.1266 mL 0.2531 mL 0.6329 mL
100 mM 0.0203 mL 0.1013 mL 0.2025 mL 0.5063 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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8-CPT-Cyclic AMP sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-CPT-Cyclic AMP93882-12-38-CPT-cAMP8-(p-Chlorophenylthio)-cAMPPKAPhosphodiesterase (PDE)Protein kinase AcyclicAMPphosphodiesterasea-FasneutrophildeathInhibitorinhibitorinhibit

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