Cat. No.: HY-112642

9-Methoxycanthin-6-one

Customer Review

Based on 1 Customer Validation

9-Methoxycanthin-6-one, a canthin-6-one alkaloid, is present in intact plant parts and in callus tissues of different explants. 9-Methoxycanthin-6-one shows anti-tumor activity, inhibits LPS-induced TNF-α and IL-1β.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 74991-91-6
Formula: C₁₅H₁₀N₂O₂
Molecular Weight:250.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

9-Methoxycanthin-6-one Chemical Structure

CAS No.: 74991-91-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All TNF Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	<2.5 μg/mL Compound: 4	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 14575431]
Breast carcinoma cell	IC₅₀	1.4 μg/mL Compound: 29	Cytotoxicity against human Breast cancer cell Cytotoxicity against human Breast cancer cell	[PMID: 34332400]
Calu-3	CC₅₀	>100 μM Compound: Table 1, R12C2	Cytotoxicity against human Calu-3 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against human Calu-3 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
Colon cancer cell line	IC₅₀	1.4 μg/mL Compound: 29	Cytotoxicity against human Colon cancer cell line Cytotoxicity against human Colon cancer cell line	[PMID: 34332400]
Fibrosarcoma cell line	IC₅₀	1.4 μg/mL Compound: 29	Cytotoxicity against human Fibrosarcoma cell line Cytotoxicity against human Fibrosarcoma cell line	[PMID: 34332400]
HCT-8	CC₅₀	21.08 μM Compound: Table 1, R12C2	Cytotoxicity against human HCT-8 cells assessed as redcution in cell viability incubated for 96 hrs by PrestoBlue reagent based assay Cytotoxicity against human HCT-8 cells assessed as redcution in cell viability incubated for 96 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
HepG2	CC₅₀	>100 μM Compound: Table 1, R12C2	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
KB	IC₅₀	1.4 μg/mL Compound: 29	Cytotoxicity against human Vincristine-resistant KB cells Cytotoxicity against human Vincristine-resistant KB cells	[PMID: 34332400]
Lung cancer cell line	IC₅₀	1.4 μg/mL Compound: 29	Cytotoxicity against human Lung cancer cell line Cytotoxicity against human Lung cancer cell line	[PMID: 34332400]
MCF7	ED₅₀	4.5 μg/mL Compound: 4	Cytotoxicity against human MCF7 cells by SRB assay Cytotoxicity against human MCF7 cells by SRB assay	[PMID: 14575431]
Melanoma cell line	IC₅₀	1.4 μg/mL Compound: 29	Cytotoxicity against human Melanoma cell line Cytotoxicity against human Melanoma cell line	[PMID: 34332400]
MRC5	CC₅₀	>100 μM Compound: Table 1, R12C2	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]
P388	ED₅₀	1.4 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 1800638]
Vero	CC₅₀	>100 μM Compound: Table 1, R12C2	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 48 hrs by PrestoBlue reagent based assay	[PMID: 36399766]

In Vitro

9-Methoxycanthin-6-one is isolated from (Eurycoma longifolia Jack) Tongkat Ali Jack explants with emphasis on the tap and fibrous roots^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 74991-91-6
Appearance Solid
Molecular Weight 250.25
Formula C₁₅H₁₀N₂O₂
Color Light yellow to yellow
SMILES

O=C1N2C3=C(N=CC=C3C4=C2C=C(OC)C=C4)C=C1
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (49.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9960 mL

19.9800 mL

39.9600 mL

5 mM

0.7992 mL

3.9960 mL

7.9920 mL

10 mM

0.3996 mL

1.9980 mL

3.9960 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Maziah M, et al. The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali. Methods Mol Biol. 2009;547:359-69. [Content Brief]

[2]. Tripetch Kanchanapoom , et al. Canthin-6-one and b-carboline alkaloids from Eurycoma harmandiana. Phytochemistry 56 (2001) 383-386.

[3]. Zhang Z, et al. Canthin-6-Ones: Potential Drugs for Chronic Inflammatory Diseases by Targeting Multiple Inflammatory Mediators. Molecules. 2023 Apr 11;28(8):3381. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9960 mL	19.9800 mL	39.9600 mL	99.9001 mL
	5 mM	0.7992 mL	3.9960 mL	7.9920 mL	19.9800 mL
	10 mM	0.3996 mL	1.9980 mL	3.9960 mL	9.9900 mL
	15 mM	0.2664 mL	1.3320 mL	2.6640 mL	6.6600 mL
	20 mM	0.1998 mL	0.9990 mL	1.9980 mL	4.9950 mL
	25 mM	0.1598 mL	0.7992 mL	1.5984 mL	3.9960 mL
	30 mM	0.1332 mL	0.6660 mL	1.3320 mL	3.3300 mL
	40 mM	0.0999 mL	0.4995 mL	0.9990 mL	2.4975 mL

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9-Methoxycanthin-6-one

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All TNF Receptor Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

9-Methoxycanthin-6-one Related Classifications

HY-112642 Related Classifications