Elaiophylin
Based on 1 Customer Validation
Elaiophylin (Azalomycin B; Gopalamicin; Efomycin E) is an autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 37318-06-2
- Formula: C54H88O18
- Molecular Weight:1025.27
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
All Antibiotic Isoforms
More
Biological Activity
Autophagy[1]
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| DU-145 | IC50 |
31 nM
Compound: 1
|
Cytotoxicity against human DU145 cells assessed as reduction in cell viability incubated for 24 hrs by FMCA method
Cytotoxicity against human DU145 cells assessed as reduction in cell viability incubated for 24 hrs by FMCA method
|
[PMID: 31079965] |
| HEK293 | IC50 |
0.2 μM
Compound: 1
|
Cytotoxicity against HEK293 cells assessed as reduction in cell viability incubated for 24 hrs by FMCA method
Cytotoxicity against HEK293 cells assessed as reduction in cell viability incubated for 24 hrs by FMCA method
|
[PMID: 31079965] |
| HeLa | IC50 |
0.19 μM
Compound: 150
|
Cytotoxicity against human HeLa cells assessed as cell growth inhibition
Cytotoxicity against human HeLa cells assessed as cell growth inhibition
|
[PMID: 35063731] |
| Huh-7 | IC50 |
4.2 nM
Compound: 1
|
Cytotoxicity against human HuH7 cells assessed as reduction in cell viability incubated for 24 hrs by FMCA method
Cytotoxicity against human HuH7 cells assessed as reduction in cell viability incubated for 24 hrs by FMCA method
|
[PMID: 31079965] |
| MCF7 | IC50 |
0.19 μM
Compound: 150
|
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition
|
[PMID: 35063731] |
Elaiophylin-mediated autophagy inhibition and lysosomal dysfunction affect ovarian cancer cell survival during hypoxia. Exposure to Elaiophylin (0.025-0.5 μM; 24 hours) causes a significant increase in ovarian cancer SKOV3 cell death in hypoxia conditions[1].
In both the SKOV3 and A2780 cell lines, Elaiophylin (0.25, 0.5, 0.75 μM; 24 hours) treatment leads to significant activation of cleaved CASP9/caspase-9 and PARP1 and downregulation of BIRC5/survivin in a concentration-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Ovarian cancer SKOV3 cells.
-
Concentration:0.025, 0.05, 0.1, 0.2, 0.5 μM
-
Incubation Time:24 hours
-
Result:Caused a significant increase in ovarian cancer SKOV3 cells death in hypoxia conditions.
-
Cell Line:Ovarian cancer SKOV3 cells; A2780 cells
-
Concentration:0.25, 0.5, 0.75 μM
-
Incubation Time:24 hours
-
Result:Treatment led to significant activation of cleaved CASP9/caspase-9 and PARP1 and downregulation of BIRC5/survivin in a concentration-dependent manner.
Lower doses of Elaiophylin as a single agent exert significant antitumor activity, while higher doses lead to intestinal toxicity. Administration of a lower dose (2 mg/kg) of Elaiophylin as a single agent achieves a significant antitumor effect without toxicity in an orthotopic ovarian cancer model with metastasis. Toxic reactions are observed only in the 8 mg/kg group[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:4-wk-old BALB/C athymic mice with ovarian cancer SKOV3 cells[1]
-
Dosage:1 or 2 mg/kg
-
Administration:Given i.p. every 2 days for 21 days
-
Result:Treatment with 2 mg/kg significantly suppressed ovarian cancer SKOV3 cells growth compared with DMSO treatment.
Chemical Information
-
CAS No. 37318-06-2
-
Appearance Solid
-
Molecular Weight 1025.27
-
Formula C54H88O18
-
Color White to off-white
-
SMILES
C[C@H]1[C@@H](O)[C@@H](O)C[C@H](O[C@@H]2C[C@@]([C@H]([C@H](O)[C@H](C)[C@](OC(/C=C/C=C/[C@H](C)[C@]([C@@H](C)[C@@H](O)[C@@H]([C@]3(O)O[C@H](C)[C@@H](CC)[C@H](O[C@H]4C[C@H](O)[C@H](O)[C@H](C)O4)C3)C)([H])OC5=O)=O)([H])[C@H](/C=C/C=C/5)C)C)(O)O[C@H](C)[C@H]2CC)O1
-
Synonyms
Azalomycin B; Gopalamicin; Efomycin E
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 20 mg/mL (19.51 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (2.44 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (282 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9754 mL | 4.8768 mL | 9.7535 mL | 24.3838 mL |
| 5 mM | 0.1951 mL | 0.9754 mL | 1.9507 mL | 4.8768 mL | |
| 10 mM | 0.0975 mL | 0.4877 mL | 0.9754 mL | 2.4384 mL | |
| 15 mM | 0.0650 mL | 0.3251 mL | 0.6502 mL | 1.6256 mL |
Elaiophylin Related Classifications
HY-15184 Related Classifications
Powered by Bioz