2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Methylmalonic acid

Methylmalonic acid  (Synonyms: Methylpropanedioic acid; Methylmalonate)

Cat. No.: HY-103395 Purity: 98.0%
COA
SDS
Handling Instructions Technical Support

Methylmalonic acid (Methylmalonate) is an indicator of Vitamin B-12 deficiency in cancer.

For research use only. We do not sell to patients.

CAS No. : 516-05-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Methylmalonic acid:

Methylmalonic acid Related Antibodies

Top Publications Citing Use of Products
Bio/Physico-chemical Assay
Cell Imaging/Staining
Cell Proliferation/Viability Assay

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Genom. 2025 Apr 24:100855.  [Abstract]

    Relative abundance of methylmalonate in hepatocytes. CON, control; OE, adenovirus-based Acsf3 overexpression; MMA, methylmalonic acid.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Genom. 2025 Apr 24:100855.  [Abstract]

    Respiratory rate of methylmalonic acid in hepatocytes. CON, control group; OE, adenovirus-based Acsf3 overexpression group; MMA, methylmalonic acid.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Genom. 2025 Apr 24:100855.  [Abstract]

    Respiratory rates in hepatocytes. MMA, 5 mM methylmalonate. N = 3. CON, control mice (Acsf3+/−); KO, knockout mice (Acsf3−/−).

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    In vitro, AC16 cells were treated with Methylmalonic acid (MMA, 17.5 mM) for 3 h and employed for further analyses. Morphology was evaluated by light microscopy after AC16 cells were treated with MMA.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    In vitro, AC16 cells were treated with Methylmalonic acid (MMA, 17.5 mM) for 3 h and employed for further analyses. AC16 cells were treated with/without MMA in Normoxia or hypoxia-reoxygenation (H/R) condition. Cell viability was detected by 3-(4,5-dimthyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT) assay.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    In vitro, AC16 cells were treated with Methylmalonic acid (MMA, 17.5 mM) for 3 h and employed for further analyses. Representative TEM images of AC16 cells treated with/without MMA. Yellow arrows indicate mitochondria.

    Methylmalonic acid purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Jan 18;22(1):53.  [Abstract]

    Methylmalonic acid (MMA)-mediated ROS contributes to cardiomyocytes injury. In vitro, AC16 cells were treated with MMA (17.5 mM) for 3 h or H2O2 (600 μM) for 6 h and then used for further analyses. ROS production was observed by DHE and DCFH-DA staining.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Methylmalonic acid (Methylmalonate) is an indicator of Vitamin B-12 deficiency in cancer.

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Vitamin B-12 is required for Methylmalonate (Methylmalonic acid; MMA) to be converted to succinyl-CoA, and in combination with folic acid, for homocysteine (HC) to be converted to methionine. Therefore, Methylmalonate is clearly more specific to vitamin B-12 deficiency compared to HC[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Methylmalonic acid Related Antibodies
    Clinical Trial
    Molecular Weight

    118.09

    Formula

    C4H6O4
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O)C(C)C(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (846.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (846.81 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 8.4681 mL 42.3406 mL 84.6812 mL
    5 mM 1.6936 mL 8.4681 mL 16.9362 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (21.17 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (21.17 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (846.81 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (394 KB)

    COA (244 KB) HNMR (227 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 8.4681 mL 42.3406 mL 84.6812 mL 211.7029 mL
    5 mM 1.6936 mL 8.4681 mL 16.9362 mL 42.3406 mL
    10 mM 0.8468 mL 4.2341 mL 8.4681 mL 21.1703 mL
    15 mM 0.5645 mL 2.8227 mL 5.6454 mL 14.1135 mL
    20 mM 0.4234 mL 2.1170 mL 4.2341 mL 10.5851 mL
    25 mM 0.3387 mL 1.6936 mL 3.3872 mL 8.4681 mL
    30 mM 0.2823 mL 1.4114 mL 2.8227 mL 7.0568 mL
    40 mM 0.2117 mL 1.0585 mL 2.1170 mL 5.2926 mL
    50 mM 0.1694 mL 0.8468 mL 1.6936 mL 4.2341 mL
    60 mM 0.1411 mL 0.7057 mL 1.4114 mL 3.5284 mL
    80 mM 0.1059 mL 0.5293 mL 1.0585 mL 2.6463 mL
    100 mM 0.0847 mL 0.4234 mL 0.8468 mL 2.1170 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Methylmalonic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methylmalonic acid516-05-2Methylpropanedioic acid MethylmalonateEndogenous MetaboliteInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Methylmalonic acid
    Cat. No.:
    HY-103395
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (394 KB) Français - FR (394 KB) Deutsch - DE (394 KB) Norwegian - NO (394 KB) Español - ES (394 KB) Swedish - SV (394 KB) Italian - IT (394 KB) Korean - KR (394 KB) Portuguese - PT (394 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.