2. Membrane Transporter/Ion Channel
    Autophagy
  3. Potassium Channel
    Autophagy
  4. NS1643

NS1643 

Cat. No.: HY-16916 Purity: 99.14%
COA Handling Instructions

NS1643 is a partial agonist of human ether-a-go-go-related gene (hERG) K+ channels with an EC50 of 10.5 μM. NS1643 inhibits the growth of breast cancer tumors in TNBC mouse models. NS1643 inhibits cell migration and invasion of breast cancer cells.

For research use only. We do not sell to patients.

NS1643 Chemical Structure

NS1643 Chemical Structure

CAS No. : 448895-37-2

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10 mM * 1 mL in DMSO USD 79 In-stock
Estimated Time of Arrival: December 31
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Description

NS1643 is a partial agonist of human ether-a-go-go-related gene (hERG) K+ channels with an EC50 of 10.5 μM. NS1643 inhibits the growth of breast cancer tumors in TNBC mouse models. NS1643 inhibits cell migration and invasion of breast cancer cells[1][2].

IC50 & Target

EC50: 10.5 μM (hERG) K+ channel[1]
In Vitro

NS1643 (0-100 μM) dose- and voltage-dependently increases hERG current in oocytes[1].
NS1643 (3, 10 and 30 μM) slows the rate of hERG inactivation throughout the testing voltage range and reduces the extent of hERG channel rectification[1].
NS1643 (10 and 50 μM) dose-dependently inhibits cell migration and invasion of MDA-MB-231 and SKBR3 cancer cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: MDA-MB-231, SKBR3 and MCF7 breast cancer cell lines
Concentration: 50 μM
Incubation Time: 24 h
Result: Decreased Vimentin, N-cadherin and CD44 levels, and increased E-cadherin in breast cancer cell lines.
In Vivo

NS1643 (6 mg/kg; i.p., twice per week) inhibits breast tumor metastasis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NSG mice with human-derived TNBC tumor xenografts[2]
Dosage: 6 mg/kg
Administration: Intraperitoneal injection; 6 mg/kg; twice per week
Result: Significantly reduceed tumor growth and the metastatic liver tumors were significantly smaller than those in the control group. Decreased levels of human nuclear antigen (HNA).
Molecular Weight

380.24

Appearance

Solid

Formula

C15H10F6N2O3
CAS No.
SMILES

O=C(NC1=C(O)C=CC(C(F)(F)F)=C1)NC2=C(O)C=CC(C(F)(F)F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (262.99 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6299 mL 13.1496 mL 26.2992 mL
5 mM 0.5260 mL 2.6299 mL 5.2598 mL
10 mM 0.2630 mL 1.3150 mL 2.6299 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.14%

COA (252 KB) HNMR (220 KB) RP-HPLC (264 KB) LCMS (104 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Keywords:

NS1643448895-37-2NS 1643NS-1643Potassium ChannelAutophagyKcsATNBCoocytemigrationinvasionMDA-MB-231SKBR3MCF7Inhibitorinhibitorinhibit

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