2. Anti-infection
  3. Parasite Antibiotic
  4. Pafuramidine

Pafuramidine (DB289) is an orally active proagent of Furamidine (HY-110137A). Pafuramidine is a potent anti-parasitic agent, can be used to research trypanosomiasis, Pneumocystis pneumonia and malaria.

For research use only. We do not sell to patients.

Pafuramidine Chemical Structure

Pafuramidine Chemical Structure

CAS No. : 186953-56-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 330 In-stock
Solution
10 mM * 1 mL in DMSO USD 330 In-stock
Solid
5 mg USD 300 In-stock
10 mg USD 456 In-stock
50 mg USD 1080 In-stock
100 mg USD 1680 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Pafuramidine:

Pafuramidine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Pafuramidine

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

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  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Pafuramidine (DB289) is an orally active proagent of Furamidine (HY-110137A). Pafuramidine is a potent anti-parasitic agent, can be used to research trypanosomiasis, Pneumocystis pneumonia and malaria[1][2][3].

IC50 & Target[1][3]

Trypanosoma

 

Plasmodium falciparum sporozoites

 

In Vivo

Pafuramidine (1-10 mg/kg; p.o.; for 5 or 10 days) cures some trypanosomes-infected monkeys[1].
Pafuramidine (2.5-100 mg/kg; p.o.; for 4 or 5 days) cures some Trypanosoma brucei-infected mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Vervet monkeys (infected by intravenous injection of 104 trypanosomes)[1]
Dosage: 1, 3, and 10 mg/kg (group 1/2/3); 10 mg/kg (group 4/5)
Administration: p.o.; 5 consecutive days for group 1/2/3 (started on the 7th day postinfection); 10 days for group 4 (started on the 14th day postinfection); 10 days for group 5 (started on the 28th day postinfection). Post-treatment monitoring was maintained for a period of 180 days.
Result: Cured all three monkeys in group 3 at 10 mg/kg, and did not recur during the monitoring period.
All three monkeys in group 4 became aparasitemic by day 5 of dosing, but only two of three monkeys remained free of blood parasites until 180 days post-treatment monitoring.
All three monkeys in group 5 became aparasitemic by day 4 of dosing, but only two of three of monkeys remained free of blood trypanosomes by the end of the 180 days of post-treatment monitoring.
Animal Model: Female NMRI mice (infected intraperitoneally with 2 × 104 STIB900 bloodstream forms)[5]
Dosage: 2.5, 5, 25 and 50 mg/kg
Administration: p.o.; for 4 days (started on the 4th day postinfection)
Result: Cured all four mice at 25 and 50 mg/kg.
Animal Model: Female NMRI mice (infected intraperitoneally with 2 × 104 GVR35 bloodstream forms)[5]
Dosage: 25, 50 and 100 mg/kg
Administration: p.o.; for 5 days (started on the 21st day postinfection)
Result: Showed good CNS activity in the GVR35 CNS model, with 3/5 mice cured at 100 mg/kg.
Clinical Trial
Molecular Weight

364.40

Formula

C20H20N4O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

N=C(C1=CC=C(C2=CC=C(C3=CC=C(C(NOC)=N)C=C3)O2)C=C1)NOC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (91.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7442 mL 13.7212 mL 27.4424 mL
5 mM 0.5488 mL 2.7442 mL 5.4885 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.21%

COA (246 KB) LCMS (267 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7442 mL 13.7212 mL 27.4424 mL 68.6059 mL
5 mM 0.5488 mL 2.7442 mL 5.4885 mL 13.7212 mL
10 mM 0.2744 mL 1.3721 mL 2.7442 mL 6.8606 mL
15 mM 0.1829 mL 0.9147 mL 1.8295 mL 4.5737 mL
20 mM 0.1372 mL 0.6861 mL 1.3721 mL 3.4303 mL
25 mM 0.1098 mL 0.5488 mL 1.0977 mL 2.7442 mL
30 mM 0.0915 mL 0.4574 mL 0.9147 mL 2.2869 mL
40 mM 0.0686 mL 0.3430 mL 0.6861 mL 1.7151 mL
50 mM 0.0549 mL 0.2744 mL 0.5488 mL 1.3721 mL
60 mM 0.0457 mL 0.2287 mL 0.4574 mL 1.1434 mL
80 mM 0.0343 mL 0.1715 mL 0.3430 mL 0.8576 mL
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Pafuramidine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pafuramidine186953-56-0DB289DB 289DB-289ParasiteAntibioticFuramidineanti-parasitictrypanosomiasisPneumocystismalariaTrypanosoma bruceiVervet monkeysNMRI miceSTIB900GVR35Inhibitorinhibitorinhibit

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