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QS11 

Cat. No.: HY-12762 Purity: 99.22%
COA Handling Instructions

QS11 is an inhibitor of ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1), with an EC50 of 1.5 µM. QS11 modulates Wnt/β-catenin signaling through an effect on protein trafficking. QS11 inhibits migration of ARFGAP overexpressing breast cancer cells.

For research use only. We do not sell to patients.

QS11 Chemical Structure

QS11 Chemical Structure

CAS No. : 944328-88-5

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 135 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 135 In-stock
Solid
5 mg USD 108 In-stock
10 mg USD 192 In-stock
25 mg USD 432 In-stock
50 mg USD 768 In-stock
100 mg USD 1320 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE QS11

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

QS11 is an inhibitor of ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1), with an EC50 of 1.5 µM. QS11 modulates Wnt/β-catenin signaling through an effect on protein trafficking. QS11 inhibits migration of ARFGAP overexpressing breast cancer cells[1].

IC50 & Target

EC50: 1.5 µM (ARFGAP1)[1]
In Vitro

QS11 binds and inhibits the GTPase activating protein of ARFGAP1, suggesting that QS11 modulates Wnt/β-catenin signaling through an effect on protein trafficking[1].
QS11 (2.5 μM) activates the Super(8X)TOPFlash reporter 200-fold in the presence of Wnt-3a conditioned medium, whereas Wnt-3a treatment alone increases reporter activity 40-fold[2].
QS11 shows potent activity (EC50=0.5 μM) with little cytotoxicity toward HEK293 and human primary fibroblast cells[2].
QS11 effectively reduces in vitro migration of metastatic human breast cancer cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

567.68

Appearance

Solid

Formula

C36H33N5O2
CAS No.
SMILES

OC[[email protected]](CC1=CC=CC=C1)NC2=C3C(N(CC4=CC=C(C5=CC=CC=C5)C=C4)C=N3)=NC(OC6=CC=C(CCC7)C7=C6)=N2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (176.16 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7616 mL 8.8078 mL 17.6156 mL
5 mM 0.3523 mL 1.7616 mL 3.5231 mL
10 mM 0.1762 mL 0.8808 mL 1.7616 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.40 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.40 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
Cell Assay
[1]

ARF-GTP levels are measured by using the GGA binding assay. NIH 3T3 cells are treated with QS11 or QS11-NC at the indicated concentrations for 36 h. Cells are lysed in ARF assay lysis buffer [50 mM Tris·HCl (pH 7.5), 100 mM NaCl, 2 mM MgCl2, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 10% glycerol, and protease inhibitors]. GTP-bound ARF is assayed by its binding to a GST fusion protein, which contains the VHS domain to the GAT region of an ARF effector GGA3[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

QS11944328-88-5QS 11QS-11OthersWntsignalingsynergisticagonistADP-ribosylationfactorsregenerativeInhibitorinhibitorinhibit

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